BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BN000925	53,069	+T	intergenic (+42/+10)	traM → / ← kfrC	TraM protein/KfrC protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BN000925	53,067	1	.	T	99.4%	640.7 / ‑4.2	162	intergenic (+40/+12)	traM/kfrC	TraM protein/KfrC protein
Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (80/81); total (81/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 167 total aligned reads displayed.

CAGCAAGGACGCAATGGCGAAGGTAATGAAGGACAGCGCCGCGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGC‑TT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCTTGGGCGCGTGAATGCCCATGCCTTCCTTG  >  BN000925/52829‑53298

cAGCAAGGACGCAATGGCGAAGGTAATGAAGGACAGCGCCGCGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCGTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGc                                                                                                                                                                                                                                           >  1:118752/1‑239 (MQ=255)

         cGCAATGGCGAAGGTAATGAAGGACAGCGCCGCGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑Gttttt                                                                                                                                                                                                                                 >  2:409323/1‑239 (MQ=255)

                gCGAAGGTAATGAAGGACAGCGCCGCGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGc                                                                                                                                                                                                                         <  1:295968/240‑1 (MQ=255)

                   aaGGTAATGAAGGACAGCGCCGCGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTgg                                                                                                                                                                                                                      <  1:365705/240‑1 (MQ=255)

                          tGAAGGACAGCGCCGCGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGtt                                                                                                                                                                                                               >  1:354570/1‑240 (MQ=255)

                              ggACAGCGCCGCGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGa                                                                                                                                                                                                            <  1:247817/239‑1 (MQ=255)

                                aCAGCGCCGCGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACa                                                                                                                                                                                                          >  1:312457/1‑239 (MQ=255)

                                aCAGCGCCGCGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAg                                                                                                                                                                                                         >  1:84694/1‑240 (MQ=255)

                                aCAGCGCCGCGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAg                                                                                                                                                                                                         >  2:202075/1‑240 (MQ=255)

                                    cgccgcGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGtt                                                                                                                                                                                                                            >  2:358593/1‑217 (MQ=255)

                                    cgccgcGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAg                                                                                                                                                                                                                          >  2:58901/1‑219 (MQ=255)

                                    cgccgcGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGccc                                                                                                                                                                                                     >  2:275199/1‑240 (MQ=255)

                                    cgccgcGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGccc                                                                                                                                                                                                     >  1:285326/1‑240 (MQ=255)

                                    cgccgcGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGccc                                                                                                                                                                                                     >  1:381423/1‑240 (MQ=255)

                                    cgccgcGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGcc                                                                                                                                                                                                      <  2:30042/239‑1 (MQ=255)

                                             ggcggcCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGcc                                                                                                                                                                                                      <  1:414039/230‑1 (MQ=255)

                                                        cGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCcgc                                                                                                                                                                                  >  1:156256/1‑239 (MQ=255)

                                                            ccGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCcgcgctcg                                                                                                                                                                             <  2:326820/240‑1 (MQ=255)

                                                            ccGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCcgcg                                                                                                                                                                                 <  1:227078/236‑1 (MQ=255)

                                                               cAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGtt                                                                                                                                                                                                               <  2:203597/203‑1 (MQ=255)

                                                                aGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGa                                                                                                                                                                                                            <  1:38795/205‑1 (MQ=255)

                                                                  ggAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTcc                                                                                                                                                                       >  1:151029/1‑240 (MQ=255)

                                                                       tCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACa                                                                                                                                                                                                          <  2:127888/200‑1 (MQ=255)

                                                                        cgacgaCGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGcc                                                                                                                                                                 <  1:409178/240‑1 (MQ=255)

                                                                        cgacgaCGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGcc                                                                                                                                                                 <  1:251355/240‑1 (MQ=255)

                                                                         gacgacGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCt                                                                                                                                                                <  1:516/240‑1 (MQ=255)

                                                                            gacgGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGct                                                                                                                                                                                                <  2:22063/205‑1 (MQ=255)

                                                                            gacgGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGt                                                                                                                                                              <  1:110534/239‑1 (MQ=255)

                                                                              cgGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGttt                                                                                                                                                            >  1:386371/1‑239 (MQ=255)

                                                                              cgGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGttt                                                                                                                                                            >  2:394664/1‑239 (MQ=255)

                                                                                   ttGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTtctc                                                                                                                                                      <  1:405153/240‑1 (MQ=255)

                                                                                           cAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCcgcgctcg                                                                                                                                                                             >  2:49868/1‑209 (MQ=255)

                                                                                           cAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTctcctgctcc                                                                                                                                               >  1:427039/1‑239 (MQ=255)

                                                                                             gCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTctcctgctcctg                                                                                                                                             >  2:234083/1‑239 (MQ=255)

                                                                                             gCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATAAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTctcctgctcctg                                                                                                                                             >  2:339881/1‑239 (MQ=255)

                                                                                                   ggCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTgcg                                                                                                                                       <  1:156419/239‑1 (MQ=255)

                                                                                                       aaGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATc                                                                                                                                   <  2:11274/239‑1 (MQ=255)

                                                                                                        aGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCa                                                                                                                                  <  2:192010/239‑1 (MQ=255)

                                                                                                             gcgGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCt                                                                                                                                                                <  2:15593/204‑1 (MQ=255)

                                                                                                             gcgGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATc                                                                                                                                   <  1:184579/233‑1 (MQ=255)

                                                                                                              cgGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCt                                                                                                                            >  1:61205/1‑239 (MQ=255)

                                                                                                               ggACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTgg                                                                                                                          <  1:191834/240‑1 (MQ=255)

                                                                                                                    cgcggcgcgTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCCCCTg                                                                                                                                                                     >  2:176633/1‑192 (MQ=255)

                                                                                                                             tGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGgctg                                                                                                             >  1:257979/1‑239 (MQ=255)

                                                                                                                                       gaACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATc                                                                                                  <  1:182169/240‑1 (MQ=255)

                                                                                                                                             gATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATcc                                                                                            >  1:257827/1‑240 (MQ=255)

                                                                                                                                              aTCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATccc                                                                                           >  1:101072/1‑240 (MQ=255)

                                                                                                                                                 gCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCACAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCt                                                                                                                                                                <  1:172974/168‑1 (MQ=255)

                                                                                                                                                  ccGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTg                                                                                                                                         >  2:26602/1‑190 (MQ=255)

                                                                                                                                                    ggcggcAGGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTg                                                                                                                                         <  2:109098/188‑1 (MQ=255)

                                                                                                                                                       ggcATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCgg                                                                                  <  2:339948/240‑1 (MQ=255)

                                                                                                                                                                  gttCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCgggg                                                                       >  2:186889/1‑239 (MQ=255)

                                                                                                                                                                   ttCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGa                                                                       >  2:49392/1‑239 (MQ=255)

                                                                                                                                                                   ttCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGAt                                                                      >  1:346148/1‑240 (MQ=255)

                                                                                                                                                                     cgcgGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGcc                                                                                >  2:365775/1‑228 (MQ=255)

                                                                                                                                                                     cgcgGCCGCCCAGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGCTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTAAc                                                                                                       >  1:111987/1‑205 (MQ=255)

                                                                                                                                                                      gctgccgccCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGCTGAAAAAGCCCGGCGTTGCCGGGC‑TTGGTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCACGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCt                                                                                                                                                                >  2:186570/4‑147 (MQ=255)

                                                                                                                                                                       cgGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCt                                                                   <  2:25372/239‑1 (MQ=255)

                                                                                                                                                                        ggCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAggt                                                                            >  2:338556/1‑227 (MQ=255)

                                                                                                                                                                        ggCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTcc                                                                 >  2:348659/1‑240 (MQ=255)

                                                                                                                                                                        ggCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTcc                                                                 >  1:127018/1‑240 (MQ=255)

                                                                                                                                                                          ccgccCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGAt                                                                      >  2:213690/1‑233 (MQ=255)

                                                                                                                                                                             cccTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCcgcgctcgcg                                                                                                                                                                           >  2:76184/1‑129 (MQ=255)

                                                                                                                                                                               cTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCcgcgcg                                                            <  2:354737/238‑1 (MQ=255)

                                                                                                                                                                                  gtggtgTGGGCCTCGTTATGAATCGCCGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCt                                                                                                                                                                      <  2:316399/129‑1 (MQ=255)

                                                                                                                                                                                       gtgGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTc                                                                                                                   >  2:273640/1‑175 (MQ=255)

                                                                                                                                                                                        tgGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGTGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCcgc                                                                                                                                                                                  >  1:315082/1‑111 (MQ=255)

                                                                                                                                                                                        tgGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTc                                                                                       >  2:285667/1‑202 (MQ=255)

                                                                                                                                                                                        tgGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCcgcgc                                                             >  2:381981/1‑228 (MQ=255)

                                                                                                                                                                                        tgGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTgcgc                                                  >  1:120483/1‑239 (MQ=255)

                                                                                                                                                                                        tgGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTgcg                                                   >  1:68628/1‑238 (MQ=255)

                                                                                                                                                                                          ggCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTc                                               >  1:305270/1‑240 (MQ=255)

                                                                                                                                                                                            ccTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACa                                                                                                                                                                                                          <  2:96508/83‑1 (MQ=255)

                                                                                                                                                                                            ccTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACa                                                                                                                                                                                                          >  1:96508/1‑83 (MQ=255)

                                                                                                                                                                                             cTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGcc                                            >  1:178997/1‑240 (MQ=255)

                                                                                                                                                                                             cTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGcc                                            >  1:349712/1‑240 (MQ=255)

                                                                                                                                                                                               cGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGa                                                                                                                                                                                                            <  2:146035/78‑1 (MQ=255)

                                                                                                                                                                                               cGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCgcgcgc                                                           >  2:68842/1‑223 (MQ=255)

                                                                                                                                                                                                          gCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGcc                                                                                                                      <  2:118373/153‑1 (MQ=255)

                                                                                                                                                                                                           cAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCtt                              >  1:129688/1‑240 (MQ=255)

                                                                                                                                                                                                           cAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCtt                              >  1:201774/1‑240 (MQ=255)

                                                                                                                                                                                                           cAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCtt                              >  1:215526/1‑240 (MQ=255)

                                                                                                                                                                                                               ggCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCTTggg                           <  1:106494/239‑1 (MQ=255)

                                                                                                                                                                                                               ggCGCAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCACCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCTTGGgc                          <  1:402199/240‑1 (MQ=255)

                                                                                                                                                                                                                   cAGATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCTTGGGcgcg                       >  2:87345/1‑239 (MQ=255)

                                                                                                                                                                                                                     gATGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTc                                    <  2:423787/224‑1 (MQ=255)

                                                                                                                                                                                                                      aTGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCTTGGGCGCGTGaa                   <  1:338414/240‑1 (MQ=255)

                                                                                                                                                                                                                       tGAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCTTGGGCGCGTGaa                   <  1:332631/239‑1 (MQ=255)

                                                                                                                                                                                                                        gAAAAAGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCTTGGGCGCGTGAATg                 <  2:33/240‑1 (MQ=255)

                                                                                                                                                                                                                          aaaaGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCTTGGGCGCGTGAATGcc               >  1:219904/1‑240 (MQ=255)

                                                                                                                                                                                                                            aaGCCCGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCACTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTgcgc                                                  <  1:25512/203‑1 (MQ=255)

                                                                                                                                                                                                                               cccGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCTTGGGCGCGTGAATGCCCATg           <  1:235725/239‑1 (MQ=255)

                                                                                                                                                                                                                               cccGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCTTGGGCGCGTGAATGCCCATg           <  1:49857/239‑1 (MQ=255)

                                                                                                                                                                                                                                ccGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAg                                                                              >  2:260717/1‑171 (MQ=255)

                                                                                                                                                                                                                                ccGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCTTGGGCGCGTGAATGCCCATGcc         >  1:298316/1‑240 (MQ=255)

                                                                                                                                                                                                                                ccGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCTTGGGCGCGTGAATGCCCATGcc         >  2:344150/1‑240 (MQ=255)

                                                                                                                                                                                                                                 cGGCGTTGCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTc                                    <  2:302019/212‑1 (MQ=255)

                                                                                                                                                                                                                                      ttGCCGGGCTTT‑GTTTTGGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTc                                    <  2:151670/207‑1 (MQ=255)

                                                                                                                                                                                                                                        gCCGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCTTGGGCGCGTGAATGCCCATGCCTTCCTTg  <  2:366099/239‑1 (MQ=255)

                                                                                                                                                                                                                                         ccGGGCTTT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGc                                                                                 <  2:218038/159‑1 (MQ=255)

CAGCAAGGACGCAATGGCGAAGGTAATGAAGGACAGCGCCGCGCAGGCGGCCGAAGCGATCCGCAGGGAAATCGACGACGGCCTTGGCCGCCAGCTCGCGGCCAAGGTCGCGGACGCGCGGCGCGTGGCGATGATGAACATGATCGCCGGCGGCATGGTGTTGTTCGCGGCCGCCCTGGTGGTGTGGGCCTCGTTATGAATCGCAGAGGCGCAGATGAAAAAGCCCGGCGTTGCCGGGC‑TT‑GTTTTTGCGTTAGCTGGGCTTGTTTGACAGGCCCAAGCTCTGACTGCGCCCGCGCTCGCGCTCCTGGGCCTGTTTCTTCTCCTGCTCCTGCTTGCGCATCAGGGCCTGGTGCCGTCGGGCTGCTTCACGCATCGAATCCCAGTCGCCGGCCAGCTCGGGATGCTCCGCGCGCATCTTGCGCGTCGCCAGTTCCTCGATCTTGGGCGCGTGAATGCCCATGCCTTCCTTG  >  BN000925/52829‑53298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`