BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BN000925	38,390	1 bp→AG	pseudogene (47/171 nt)	upf35.8 →	probable truncation of theoretical product due to sequence errors

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BN000925	38,389	1	.	A	84.4%	1056.6 / 174.6	313	pseudogene (46/171 nt)	upf35.8	probable truncation of theoretical product due to sequence errors
Reads supporting (aligned to +/- strand): ref base . (17/32); new base A (123/141); total (140/173)
Fisher's exact test for biased strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.06e-03
*	BN000925	38,390	0	T	G	84.3%	838.5 / 138.4	313	pseudogene (47/171 nt)	upf35.8	probable truncation of theoretical product due to sequence errors
Reads supporting (aligned to +/- strand): ref base T (0/1); major base G (123/140); minor base A (17/32); total (140/173)
Fisher's exact test for biased strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.14e-02

Only 100 of 313 total aligned reads displayed.

TGAATATCAAAGGAGAGAGTTACCGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGAG‑T‑CTCTACGAAGCGATCGGCATGCTCGGTTTCCTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACTGGGATGAAGAGCGCAGACCTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCATCTATCTCGACCTCCGAG  >  BN000925/38164‑38610

|||

tGAATATCAAAGGAGAGAGTTACCGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCg                                                                                                                                                                                                                  >  1:218344/1‑240 (MQ=255)

       cAAAGGAGAGAGTTACCGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCa                                                                                                                                                                                                           >  1:674507/1‑240 (MQ=255)

          aGGAGAGAGTTACCGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGc                                                                                                                                                                                                        <  1:576765/240‑1 (MQ=255)

           ggAGAGAGTTACCGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGTCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCt                                                                                                                                                                                                       <  2:216561/240‑1 (MQ=255)

           ggAGAGAGTTACCGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGc                                                                                                                                                                                                        <  2:736628/239‑1 (MQ=255)

            gagagaGTTACCGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGc                                                                                                                                                                                                            <  2:262994/234‑1 (MQ=255)

            gagagaGTTACCGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGc                                                                                                                                                                                                            >  1:262998/1‑234 (MQ=255)

            gagagaGTTACCGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTc                                                                                                                                                                                                      >  2:123004/1‑240 (MQ=255)

            gagagaGTTACCGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTc                                                                                                                                                                                                      <  2:610441/240‑1 (MQ=255)

            gagagaGTTACCGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTc                                                                                                                                                                                                      >  2:652014/1‑240 (MQ=255)

             agagagTTACCGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTc                                                                                                                                                                                                      <  2:561894/239‑1 (MQ=255)

               agagTTACCGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCgg                                                                                                                                                                                                    >  1:174187/1‑239 (MQ=255)

                 agTTACCGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGttt                                                                                                                                                                                                 >  1:288086/1‑240 (MQ=255)

                      ccGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTttcttcc                                                                                                                                                                                            >  1:593215/1‑236 (MQ=255)

                      ccGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTttcttcc                                                                                                                                                                                            <  2:40538/240‑5 (MQ=255)

                             aaaGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCa                                                                                                                                                                                    <  2:647819/240‑1 (MQ=255)

                             aaaGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCa                                                                                                                                                                                    <  2:133706/240‑1 (MQ=255)

                             aaaGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCa                                                                                                                                                                                    <  1:96/240‑1 (MQ=255)

                             aaaGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCa                                                                                                                                                                                    <  2:731919/240‑1 (MQ=255)

                              aaGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCaa                                                                                                                                                                                   <  1:440941/240‑1 (MQ=255)

                                    aaCGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGtt                                                                                                                                                                              >  2:419460/1‑239 (MQ=255)

                                        tAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGt                                                                                                                                                                                         >  2:386035/1‑224 (MQ=255)

                                         aaaGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGAc                                                                                                                                                                         <  1:605266/239‑1 (MQ=255)

                                          aaGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCt                                                                                                                                                                       <  1:419464/240‑1 (MQ=255)

                                                gaatGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGct                                                                                                                                                                  >  2:453408/4‑239 (MQ=255)

                                                     cTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAGAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCG‑AGCTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTAcc                                                                                                                                                             >  1:312730/1‑239 (MQ=255)

                                                      tGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGt                                                                                                                                                           <  1:327135/240‑1 (MQ=255)

                                                               acacAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAg                                                                                                                                                                                      >  2:692873/1‑204 (MQ=255)

                                                                    aCGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAgg                                                                                                                                              >  1:428382/1‑239 (MQ=255)

                                                                          aTCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAgcg                                                                                                                                       <  2:570822/240‑1 (MQ=255)

                                                                          aTCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAgcg                                                                                                                                       <  1:136731/240‑1 (MQ=255)

                                                                              gTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCG‑AGCTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGcgcga                                                                                                                                    <  2:638766/239‑2 (MQ=255)

                                                                               tgatgatgaAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGc                                                                                                                                                                                                                   >  2:353159/1‑160 (MQ=255)

                                                                                 atgatgaAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTg                                                                                                                                >  1:384621/1‑240 (MQ=255)

                                                                                  tgatgaAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTcg                                                                                                                                                         >  2:62499/1‑214 (MQ=255)

                                                                                    atgaAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAgg                                                                                                                             <  1:570936/240‑1 (MQ=255)

                                                                                     tgaAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAgg                                                                                                                             <  1:617592/239‑1 (MQ=255)

                                                                                         aTGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGAtt                                                                                                                        >  1:398546/1‑240 (MQ=255)

                                                                                         aTGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCG‑AGCTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGAtt                                                                                                                        >  1:254733/1‑240 (MQ=255)

                                                                                            gTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCG‑AGCTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGa                                                                                                                     <  1:453412/240‑1 (MQ=255)

                                                                                                       aCAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGAc                                                                                                          <  1:635903/240‑1 (MQ=255)

                                                                                                          gCATCAGTAACGAAAGTATCTTGACGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAgcg                                                                                                                                       <  2:320107/208‑1 (MQ=255)

                                                                                                             tCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACTggac                                                                                                     >  1:690835/1‑237 (MQ=255)

                                                                                                                    cGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGAGATTGGAAACCCTTCGACT‑GGATGAAGAgcg                                                                                            <  1:206743/240‑1 (MQ=255)

                                                                                                                     gAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGTTCTACCGt                                                                                                                                                           >  2:194064/1‑177 (MQ=255)

                                                                                                                         gTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGa                                                                                        <  1:527990/239‑1 (MQ=255)

                                                                                                                           aTCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGa                                                                                                                                                  <  1:357438/180‑1 (MQ=255)

                                                                                                                           aTCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGAc                                                                                                                                            <  2:668117/186‑1 (MQ=255)

                                                                                                                           aTCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGGGCgg                                                                                                                                    >  2:414268/1‑194 (MQ=255)

                                                                                                                            tCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCG‑AGCTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGaa                                                                                                 <  2:552750/227‑1 (MQ=255)

                                                                                                                                aGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCTGCATGCTCCGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTAGCGCAGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGTAGActagc                                                                                   <  2:308457/237‑5 (MQ=255)

                                                                                                                                aGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTATACGAAGCGATTGGCATGCTCGGTTT‑CTTCCGTGCGCAAGTGTTACGACCTTTGCTCTACCGTCGCGCTGGGAAGGACCAGCGCGGAGTGAGGCGATTGCAAACGCTTCGACT‑GGATGAag                                                                                                >  2:306031/1‑224 (MQ=255)

                                                                                                                                aGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCG‑AGCTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGccac                                                                               <  1:46794/240‑1 (MQ=255)

                                                                                                                                 gCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGccac                                                                               <  2:458999/239‑1 (MQ=255)

                                                                                                                                    ggCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCgg                                                                                                                                    >  1:730969/1‑185 (MQ=255)

                                                                                                                                    ggCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGa                                                                                        >  1:132867/1‑228 (MQ=255)

                                                                                                                                     gCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGccaccacca                                                                          <  1:100081/240‑1 (MQ=255)

                                                                                                                                      cATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCG‑AGCTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCAt                                                                         >  1:77241/1‑240 (MQ=255)

                                                                                                                                       aTGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCG‑AGCTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCAt                                                                         >  2:437819/1‑239 (MQ=255)

                                                                                                                                         gAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCG‑AGCTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTg                                                                       <  2:369730/239‑1 (MQ=255)

                                                                                                                                          aaaaTGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTgcg                                                                     <  2:494971/240‑1 (MQ=255)

                                                                                                                                          aaaaTGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTgcg                                                                     <  2:100139/240‑1 (MQ=255)

                                                                                                                                                 cAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCgcgc                                                                                                                                                      <  2:525296/154‑1 (MQ=255)

                                                                                                                                                 cAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACg                                                              <  1:375866/240‑1 (MQ=255)

                                                                                                                                                  aaaTAACGTTCAAACATCGTGGCGTTGACAACTTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGa                                                             <  1:316699/240‑1 (MQ=255)

                                                                                                                                                  aaaTAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACg                                                              <  2:655944/239‑1 (MQ=255)

                                                                                                                                                         ggTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCG‑AGCTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGtt                                                       <  1:160715/239‑1 (MQ=255)

                                                                                                                                                          gTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGAGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTtgt                                                     <  1:555139/240‑1 (MQ=255)

                                                                                                                                                            cAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCG‑AGCTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTtgtc                                                    <  2:166764/239‑1 (MQ=255)

                                                                                                                                                                  tCGTGGCGTTGACGACGTGCCTGAATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGTGAG‑CTTTACGGAGCGATCGGCATGCTCGGTGT‑CGTCCGTGAGCAATTGTTAGGACCTTTGCTCTACCGTctcga                                                                                                                                                      <  2:292451/137‑2 (MQ=255)

                                                                                                                                                                    gTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTtgt                                                     <  2:686821/230‑1 (MQ=255)

                                                                                                                                                                     tGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAT‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGa                                                                                                                     <  2:724792/167‑1 (MQ=255)

                                                                                                                                                                     tGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGa                                           >  1:365824/1‑239 (MQ=255)

                                                                                                                                                                                 cGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGccac                                                                               >  2:334422/1‑191 (MQ=255)

                                                                                                                                                                                  gTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCAt                             <  1:433501/240‑1 (MQ=255)

                                                                                                                                                                                    gCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATc                            >  2:373015/1‑239 (MQ=255)

                                                                                                                                                                                        ggATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGccac                                                                               <  2:228255/184‑1 (MQ=255)

                                                                                                                                                                                            cTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCg                                                         <  2:257117/202‑1 (MQ=255)

                                                                                                                                                                                            cTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCat                   <  1:684458/240‑1 (MQ=255)

                                                                                                                                                                                            cTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCa                    <  1:131761/239‑1 (MQ=255)

                                                                                                                                                                                              ggCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCatct                 >  1:111473/1‑240 (MQ=255)

                                                                                                                                                                                              ggCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCatct                 >  1:393713/1‑240 (MQ=255)

                                                                                                                                                                                              ggCTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCatc                  >  1:82873/1‑239 (MQ=255)

                                                                                                                                                                                                cTACACTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCatctat               >  2:149864/1‑240 (MQ=255)

                                                                                                                                                                                                   cacTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAg                                              >  2:568480/1‑206 (MQ=255)

                                                                                                                                                                                                    acTATGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCATCTAtctc            >  2:88201/1‑239 (MQ=255)

                                                                                                                                                                                                    acTATGCGGCCACCAAGCTCGCCCGTGGCG‑AGCTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCATCTATCTCg           >  1:649371/1‑240 (MQ=255)

                                                                                                                                                                                                        tGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCgg                                                                                                                                                                                                             >  2:589654/1‑45 (MQ=255)

                                                                                                                                                                                                        tGCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCATCTATCTCGAcc        >  2:559253/1‑239 (MQ=255)

                                                                                                                                                                                                        tGCGGCCACCAAGCTCGCCCGTGGCG‑AGCTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCa                                                                >  2:678851/1‑183 (MQ=255)

                                                                                                                                                                                                         gCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCGGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCatctat               >  2:362499/1‑231 (MQ=255)

                                                                                                                                                                                                         gCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGccaccacca                                                                          >  2:539669/1‑172 (MQ=255)

                                                                                                                                                                                                         gCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGccaccacca                                                                          <  1:539673/172‑1 (MQ=255)

                                                                                                                                                                                                         gCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCATCTATCTCGACCTc      >  1:508768/1‑240 (MQ=255)

                                                                                                                                                                                                         gCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCATCTATCTCGACCTc      >  1:645457/1‑240 (MQ=255)

                                                                                                                                                                                                         gCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCATCTATCTCGACCTc      >  1:350932/1‑240 (MQ=255)

                                                                                                                                                                                                         gCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCATCTATCTCGACCTc      >  1:185912/1‑240 (MQ=255)

                                                                                                                                                                                                         gCGGCCACCAAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCATCTATCTCGACCTc      >  1:152860/1‑240 (MQ=255)

                                                                                                                                                                                                             ccaccaAGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCATCTATCTCGACCTCCGAg  <  1:463967/240‑1 (MQ=255)

                                                                                                                                                                                                                   aGCTCGCCCGTGGCGAG‑CTTTACGAAGCGATCGGCATGCTCGGTTT‑CTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACT‑GGATGAAGAGCGCAGA‑CTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGcc                        <  2:243314/212‑1 (MQ=255)

|||

TGAATATCAAAGGAGAGAGTTACCGGTTAAAAGAGAAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTAACGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGTGCCTGGATCTGGCTACACTATGCGGCCACCAAGCTCGCCCGTGGAG‑T‑CTCTACGAAGCGATCGGCATGCTCGGTTTCCTTCCGTGAGCAAGTGTTAGGACCTTTGCTCTACCGTCGCGCTGGAAAGGACCAGCGCGGAGTGAGGCGATTGGAAACCCTTCGACTGGGATGAAGAGCGCAGACCTAGCCACCACCATTGCGCTGCACGATGCGTTGTCTGTCAGGGATGCCATCAAAGCATCTGCCTCCATCTATCTCGACCTCCGAG  >  BN000925/38164‑38610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`