Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,695,585	(G)7→8	intergenic (‑42/‑216)	purL ← / → mltF	phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,695,578	1	.	G	100.0%	193.6 / NA	53	intergenic (‑35/‑223)	purL/mltF	phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (22/31);  total (22/31)

CCGGGCCATATTTCAGCAGGCGTTCAAGTTGTGCGTGCTCATCATCGTTTAACGGCGCATTGAGGTCAGCAAAATGGACATACTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG  >  NC_000913/2695354‑2695803                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                   ccGGGCCATATTTCAGCAGGCGTTCAAGTTGTGCGTGCTCATCATCGTTTAACGGCGCATTGAGGTCAGCAAAATGGACATACTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACggg                                                                                                                                                                                                                     <  1:367001/240‑1 (MQ=255)   gggCCATATTTCAGCAGGCGTTCAAGTTGTGCGTGCTCATCATCGTTTAACGGCGCATTGAGGTCAGCAAAATGGACATACTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCg                                                                                                                                                                                                                   <  1:480925/240‑1 (MQ=255)               agcagGCGTTCAAGTTGTGCGTGCTCATCATCGTTTAACGGCGCATTGAGGTCAGCAAAATGGACATACTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAg                                                                                                                                                                                                        >  2:245901/1‑239 (MQ=255)                         cAAGTTGTGCGTGCTCATCATCGTTTAACGGCGCATTGAGGTCAGCAAAATGGACATACTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGAAAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATgcg                                                                                                                                                                                             >  1:673397/1‑240 (MQ=255)                                  cgtgcTCATCATCGTTTAACGGCGCATTGAGGTCAGCAAAATGGACATACTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGaaa                                                                                                                                                                                    >  1:626026/1‑240 (MQ=255)                                  cgtgcTCATCATCGTTTAACGGCGCATTGAGGTCAGCAAAATGGACATACTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGaaa                                                                                                                                                                                    >  2:139589/1‑240 (MQ=255)                                          tcatcGTTTAACGGCGCATTGAGGTCAGCAAAATGGACATACTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTg                                                                                                                                                                             >  1:526956/1‑239 (MQ=255)                                           catcGTTTAACGGCGCATTGAGGTCAGCAAAATGGACATACTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑ggggggggaaa                                                                                                                                                                                                                        <  2:543677/194‑5 (MQ=255)                                                tttAACGGCGCATTGAGGTCAGCAAAATGGACATACTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGaa                                                                                                                                                                      >  1:145841/1‑240 (MQ=255)                                                             tGAGGTCAGCAAAATGGACATACTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCAt                                                                                                                                                         <  2:673981/240‑1 (MQ=255)                                                                   cAGCAAAATGGACATACTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGaa                                                                                                                                                   <  1:139591/240‑1 (MQ=255)                                                                                      ggCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑gg                                                                                                                                                                                                                                 >  1:146736/1‑142 (MQ=255)                                                                                        cGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCaa                                                                                                                               >  1:591438/1‑239 (MQ=255)                                                                                        cGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCATCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAAt                                                                                                                              >  2:474432/1‑240 (MQ=255)                                                                                          tAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATgg                                                                                                                            <  1:616512/240‑1 (MQ=255)                                                                                           aaaTATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATgg                                                                                                                            <  1:197846/239‑1 (MQ=255)                                                                                           aaaTATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATgg                                                                                                                            <  1:476052/239‑1 (MQ=255)                                                                                                  gtgAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATgg                                                                                                                            >  1:717055/1‑232 (MQ=255)                                                                                                  gtgAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATgg                                                                                                                            <  2:717042/232‑1 (MQ=255)                                                                                                  gtgAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGtaata                                                                                                                    >  1:153719/1‑240 (MQ=255)                                                                                                    gAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGtaataa                                                                                                                   >  1:444142/1‑239 (MQ=255)                                                                                                       ccGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAAc                                                                                                                                           >  2:461351/1‑212 (MQ=255)                                                                                                                 ggCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGgcg                                                                                                     <  2:142967/240‑1 (MQ=255)                                                                                                                 ggCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGgcg                                                                                                     <  2:712596/240‑1 (MQ=255)                                                                                                                                 gCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCaaaa                                                                                      <  1:204466/240‑1 (MQ=255)                                                                                                                                  ccAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATg                                                                                    <  1:281328/240‑1 (MQ=255)                                                                                                                                   cagcagTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAATTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGgcg                                                                                                     <  1:100798/222‑1 (MQ=255)                                                                                                                                        gTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCt                                                                               <  2:153717/239‑1 (MQ=255)                                                                                                                                             ttgATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATc                                                                                                          <  1:456081/207‑1 (MQ=255)                                                                                                                                                          ccGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTgccgcc                                                                                                                                 <  1:682867/171‑1 (MQ=255)                                                                                                                                                           cGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCg                                                                                 <  1:265488/218‑1 (MQ=255)                                                                                                                                                             aCAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCa                                                          <  1:320904/239‑1 (MQ=255)                                                                                                                                                             aCAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCAc                                                         <  1:307692/240‑1 (MQ=255)                                                                                                                                                               aGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCAc                                                         <  1:275066/239‑1 (MQ=255)                                                                                                                                                                   cAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACtattat                                                   >  1:703038/1‑240 (MQ=255)                                                                                                                                                                       cGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACg                                               <  1:431051/240‑1 (MQ=255)                                                                                                                                                                           ccACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCaa                                                                                                                               >  2:411346/1‑156 (MQ=255)                                                                                                                                                                           ccACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTaaa                                                                 >  1:225947/1‑218 (MQ=255)                                                                                                                                                                           ccACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCttt                                                            >  1:513840/1‑223 (MQ=255)                                                                                                                                                                           ccACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCttt                                                            <  2:513831/223‑1 (MQ=255)                                                                                                                                                                              cGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGATTACGCAAACTGCCGc                                                                                >  2:520117/1‑200 (MQ=255)                                                                                                                                                                                   aaTTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCt                                   <  1:695767/240‑1 (MQ=255)                                                                                                                                                                                     tttCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTgg                                 >  2:640017/1‑240 (MQ=255)                                                                                                                                                                                           tcatAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTg                                  >  2:126606/1‑233 (MQ=255)                                                                                                                                                                                             atAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCa                         <  1:192624/240‑1 (MQ=255)                                                                                                                                                                                             atAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑gggg                                                                                                                                                                                                                               >  2:189559/1‑41 (MQ=255)                                                                                                                                                                                              tAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCa                         <  2:709363/239‑1 (MQ=255)                                                                                                                                                                                                   ctctcGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGacac                   <  2:569585/240‑1 (MQ=255)                                                                                                                                                                                                      tcGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCACCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGAcaca                  <  1:690520/238‑1 (MQ=255)                                                                                                                                                                                                       cGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGgcg                                                                                                     <  2:52614/154‑1 (MQ=255)                                                                                                                                                                                                        gTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCa                         <  1:608816/229‑1 (MQ=255)                                                                                                                                                                                                          cttctAAGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCa                         <  1:571105/227‑1 (MQ=255)                                                                                                                                                                                                                aGCTTTCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCACTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTa      >  2:264791/1‑240 (MQ=255)                                                                                                                                                                                                                     tCGGTGTACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTg  >  2:35386/1‑239 (MQ=255)                                                                                                                                                                                                                          gtACCCAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGAcaca                  <  2:86559/218‑1 (MQ=255)                                                                                                                                                                                                                             cccAAG‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTacac                                                                >  2:372935/1‑169 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                   CCGGGCCATATTTCAGCAGGCGTTCAAGTTGTGCGTGCTCATCATCGTTTAACGGCGCATTGAGGTCAGCAAAATGGACATACTCGGCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTG  >  NC_000913/2695354‑2695803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑