Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,360,538	T→C	R447R (CGT→CGC)	gltD →	glutamate synthase subunit GltD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,360,538	0	T	C	100.0%	243.1 / NA	66	R447R (CGT→CGC)	gltD	glutamate synthase subunit GltD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/36);  total (30/36)

AACCGGACGCCAAAGGCCGTCGCCGCGCGGAGATCGTTGCAGGTTCCGAACATATCGTTCCGGCAGATGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAG  >  NC_000913/3360309‑3360771                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          aaCCGGACGCCAAAGGCCGTCGCCGCGCGGAGATCGTGGCAGGTTCCGAACATATCGTTCCGGCAGATGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGAt                                                                                                                                                                                                                                  <  2:206369/239‑1 (MQ=255)       aCGCCAAAGGCCGTCGCCGCGCGGAGATCGTTGCAGGTTCCGAACATATCGTTCCGGCAGATGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCtggtgg                                                                                                                                                                                                                           >  2:570589/1‑240 (MQ=255)              aGGCCGTCGCCGCGCGGAGATCGTTGCAGGTTCCGAACATATCGTTCCGGCAGATGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGc                                                                                                                                                                                                                    >  1:123306/1‑240 (MQ=255)                       ccgcGCGGAGATCGTTGCAGGTTCCGAACATATCGTTCCGGCAGATGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGa                                                                                                                                                                                                           <  1:73907/240‑1 (MQ=255)                                         aGGTTCCGAACATATCGTTCCGGCAGATGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGa                                                                                                                                                                                         <  1:277884/240‑1 (MQ=255)                                          ggTTCCGAACATATCGTTCCGGCAGATGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAg                                                                                                                                                                                          <  1:279373/238‑1 (MQ=255)                                          ggTTCCGAACATATCGTTCCGGCAGATGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGa                                                                                                                                                                                         <  1:222629/239‑1 (MQ=255)                                           gTTCCGAACATATCGTTCCGGCAGATGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACg                                                                                                                                                                                       >  1:209669/1‑240 (MQ=255)                                           gTTCCGAACATATCGTTCCGGCAGATGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACg                                                                                                                                                                                       <  2:489288/240‑1 (MQ=255)                                           gTTCCGAACATATCGTTCCGGCAGATGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACg                                                                                                                                                                                       <  2:341278/240‑1 (MQ=255)                                            ttCCGAACATATCGTTCCGGCAGATGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACg                                                                                                                                                                                       <  2:670377/239‑1 (MQ=255)                                                 aaCATATCGTTCCGGCAGATGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGtatt                                                                                                                                                                                  <  1:653359/239‑1 (MQ=255)                                                      atCGTTCCGGCAGATGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGaa                                                                                                                                                                             <  2:152308/239‑1 (MQ=255)                                                                 aGATGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACg                                                                                                                                                                                       <  2:431484/218‑1 (MQ=255)                                                                   aTGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTAGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGttt                                                                                                                                                               >  1:160524/1‑240 (MQ=255)                                                                    tGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTa                                                                                                                                                              >  2:474651/1‑240 (MQ=255)                                                                     gCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTaa                                                                                                                                                             >  1:235240/1‑240 (MQ=255)                                                                       ggTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAg                                                                                                                                                            <  2:449557/239‑1 (MQ=255)                                                                       ggTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAg                                                                                                                                                            <  1:284844/239‑1 (MQ=255)                                                                             cATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGa                                                                                                                                                                                         <  2:708319/204‑1 (MQ=255)                                                                                     ttGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGaa                                                                                                                                             >  1:130472/1‑240 (MQ=255)                                                                                     ttGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGaa                                                                                                                                             >  1:217110/1‑240 (MQ=255)                                                                                     ttGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGaa                                                                                                                                             >  1:56951/1‑240 (MQ=255)                                                                                      tGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGaa                                                                                                                                             >  2:56471/1‑239 (MQ=255)                                                                                      tGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAAt                                                                                                                                            >  1:45193/1‑240 (MQ=255)                                                                                          ttCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGc                                                                                                                                        <  1:301701/240‑1 (MQ=255)                                                                                              gTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGa                                                                                                                                    <  2:3230/240‑1 (MQ=255)                                                                                               tCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGa                                                                                                                                    <  1:103840/239‑1 (MQ=255)                                                                                                ccACACAACAGGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGa                                                                                                                                    <  2:232119/238‑1 (MQ=255)                                                                                                  acacaACATGGAAGGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGc                                                                                                                                        <  2:54721/232‑1 (MQ=255)                                                                                                        caTGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGAc                                                                                                                                   <  2:702699/231‑1 (MQ=255)                                                                                                        caTGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGAc                                                                                                                                   >  1:702711/1‑231 (MQ=255)                                                                                                        caTGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTgg                                                                                                                          >  2:213616/1‑240 (MQ=255)                                                                                                                ggctggcAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCaa                                                                                                                  >  2:443728/1‑240 (MQ=255)                                                                                                                  ctggcAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGt                                                                                                                                                                                                    >  2:310358/1‑156 (MQ=255)                                                                                                                  ctggcAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCa                                                                                                                   >  1:414055/1‑237 (MQ=255)                                                                                                                  ctggcAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAAt                                                                                                                >  1:611941/1‑240 (MQ=255)                                                                                                                  ctggcAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAAt                                                                                                                >  1:49423/1‑240 (MQ=255)                                                                                                                                         ggAGTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGGAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGa                                                                                                       <  2:556697/226‑1 (MQ=255)                                                                                                                                            ttCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTc                                                                                       >  2:567060/1‑239 (MQ=255)                                                                                                                                                    gCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATg                                                                              >  1:329922/1‑240 (MQ=255)                                                                                                                                                      cGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGGAGGGCGTa                                                                                                                                                                                                   >  2:577379/1‑121 (MQ=255)                                                                                                                                                         atcatcGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGc                                                                          <  1:401802/239‑1 (MQ=255)                                                                                                                                                           catcGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATg                                                                       <  2:235237/240‑1 (MQ=255)                                                                                                                                                               gCCCCGGAAGGCAGCGACAAGGCCTTCCAGACCAGCAACCCGAAAACCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAAt                                                                                                                                            <  2:641483/167‑1 (MQ=255)                                                                                                                                                                   cGGAAGGCAGCGACAACGCCTTCCAGACCAGCAAGCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTc                                                                                                                                                                                                                                      <  2:102014/73‑1 (MQ=255)                                                                                                                                                                     gAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATg                                                                                                                                                                               <  2:375410/126‑1 (MQ=255)                                                                                                                                                                     gAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCa                                                                                  <  1:214141/219‑1 (MQ=255)                                                                                                                                                                      aaGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGAc                                                             <  1:457171/239‑1 (MQ=255)                                                                                                                                                                       aGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGAcc                                                            <  2:433281/239‑1 (MQ=255)                                                                                                                                                                           aGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCa                                                                         >  1:713612/1‑222 (MQ=255)                                                                                                                                                                           aGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTcc                                                       >  1:554711/1‑240 (MQ=255)                                                                                                                                                                               aCAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCtt                                                          >  2:452208/1‑233 (MQ=255)                                                                                                                                                                               aCAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCtt                                                          <  1:452216/233‑1 (MQ=255)                                                                                                                                                                                             aCCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAAtttt                                     >  1:559910/1‑240 (MQ=255)                                                                                                                                                                                             aCCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAAttt                                      <  1:641077/239‑1 (MQ=255)                                                                                                                                                                                                  cAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAAt                                 <  1:1088/239‑1 (MQ=255)                                                                                                                                                                                                  cAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATa                                >  2:28757/1‑240 (MQ=255)                                                                                                                                                                                                  cAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATa                                >  1:375465/1‑240 (MQ=255)                                                                                                                                                                                                       cGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAAtt                            >  1:227827/1‑239 (MQ=255)                                                                                                                                                                                                       cGAAAATCTTTGCTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAAtt                            >  2:396523/1‑239 (MQ=255)                                                                                                                                                                                                                   cTGGCGGCGATATCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCAc               >  1:533316/1‑240 (MQ=255)                                                                                                                                                                                                                             tatCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtgat     <  1:230566/240‑1 (MQ=255)                                                                                                                                                                                                                              atCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtgat     <  1:455312/239‑1 (MQ=255)                                                                                                                                                                                                                               tCGTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCtgatga   <  1:227873/240‑1 (MQ=255)                                                                                                                                                                                                                                 gTCCGCGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAg  <  1:60043/239‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          AACCGGACGCCAAAGGCCGTCGCCGCGCGGAGATCGTTGCAGGTTCCGAACATATCGTTCCGGCAGATGCGGTGATCATGGCGTTTGGTTTCCGTCCACACAACATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCACAAGGCCGCATCATCGCCCCGGAAGGCAGCGACAACGCCTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTGGTGACCGCTATTGCCGAAGGTCGTAAGGCGGCAGACGGTATTATGAACTGGCTGGAAGTTTAAGCGAGGTAACAATGAATTCGCCAGACTGTGTCTGGCGAATCAAATTACTGACGAAGTGCGCGTTGTTTTCTGGCAAATGCGGCATGAGCGTTTGACCTTTCCTATAATAGGGATAATTTTCAATAAATTGTGCTTTGTTCACCAAGTCTGATGAG  >  NC_000913/3360309‑3360771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑