Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,966,398	T→C	intergenic (+66/‑19)	rhoL → / → rho	rho operon leader peptide/transcription termination factor Rho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,966,398	0	T	C	100.0%	175.2 / NA	49	intergenic (+66/‑19)	rhoL/rho	rho operon leader peptide/transcription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/26);  total (23/26)

CGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTAC  >  NC_000913/3966167‑3966632                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           cGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGaa                                                                                                                                                                                                                                     >  2:238261/1‑239 (MQ=255)       aaaCATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCacac                                                                                                                                                                                                                              <  2:653818/240‑1 (MQ=255)       aaaCATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCacac                                                                                                                                                                                                                              <  2:514024/240‑1 (MQ=255)        aaCATCCCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCacac                                                                                                                                                                                                                              <  2:16856/239‑1 (MQ=255)             aCCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACt                                                                                                                                                                                                                          >  2:717126/1‑238 (MQ=255)                                       ttCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGc                                                                                                                                                                                              <  2:52776/240‑1 (MQ=255)                                         ccACCGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGcc                                                                                                                                                                                             <  1:201270/239‑1 (MQ=255)                                            aCGCTTCCCGTTTTAACGTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATAAGTCGATGACCGTAAACATGCATGGATGATCCTGCCATACCAGTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACt                                                                                                                                                                                                                                   <  2:231196/198‑1 (MQ=255)                                             cGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGttt                                                                                                                                                                                        <  2:730704/240‑1 (MQ=255)                                                 tCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGa                                                                                                                                                                                    <  2:574042/240‑1 (MQ=255)                                                                   gCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAg                                                                                                                                                                                   <  2:34492/223‑1 (MQ=255)                                                                                    cTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTa                                                                                                                                                                                                                                         <  1:71846/152‑1 (MQ=255)                                                                                          cGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAAACTTACCGAATTAAAGAATACGcc                                                                                                                                                                                             <  2:59304/190‑1 (MQ=255)                                                                                                         cTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAg                                                                                                                             >  2:79941/1‑239 (MQ=255)                                                                                                                tttCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACa                                                                                                                      <  2:631360/239‑1 (MQ=255)                                                                                                                      ttCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATgtgt                                                                                                                                >  2:313617/1‑223 (MQ=255)                                                                                                                         tGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGc                                                                                                            <  1:79942/240‑1 (MQ=255)                                                                                                                           cGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGATCTGATCAATCTCGGc                                                                                                                                                                    >  2:383586/1‑182 (MQ=255)                                                                                                                                tctcCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTg                                                                                                     >  1:582306/1‑240 (MQ=255)                                                                                                                                  tcCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGcc                                                                                                                                                                                             >  1:556599/1‑150 (MQ=255)                                                                                                                                  tcCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGcc                                                                                                                                                                                             <  2:556590/150‑1 (MQ=255)                                                                                                                                  tcCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCtta                                                                                                    >  1:454573/1‑237 (MQ=255)                                                                                                                                   ccTGTGACCAGGCAGCGAAAAGACATGAGTCAATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTAGTGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCTTAAGCAGGACATTATTTTTGCCATCCTGAag                                                                                                  >  1:299660/1‑240 (MQ=255)                                                                                                                                     tgtgACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAagc                                                                                                 >  1:562716/1‑239 (MQ=255)                                                                                                                                      gtgACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAagcag                                                                                               <  1:161442/240‑1 (MQ=255)                                                                                                                                      gtgACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAagcag                                                                                               <  2:349987/240‑1 (MQ=255)                                                                                                                                      gtgACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAagcag                                                                                               <  2:610206/240‑1 (MQ=255)                                                                                                                                          ccAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCAc                                                                                            >  2:481918/1‑239 (MQ=255)                                                                                                                                            aGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACatt                                                                                                                    >  1:314800/1‑213 (MQ=255)                                                                                                                                              gCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGc                                                                                                            <  2:548107/219‑1 (MQ=255)                                                                                                                                              gCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCaaa                                                                                       >  1:586515/1‑240 (MQ=255)                                                                                                                                              gCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCaaa                                                                                       >  1:407287/1‑240 (MQ=255)                                                                                                                                                aGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTgg                                                                                                                                                    <  2:326730/177‑1 (MQ=255)                                                                                                                                                              aGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGata                                                                         >  1:528866/1‑238 (MQ=255)                                                                                                                                                                   aTGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTg                                                                   >  1:21487/1‑239 (MQ=255)                                                                                                                                                                   aTGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCAATATGAAGCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATgggg                                                                                                                                                        >  2:191265/1‑154 (MQ=255)                                                                                                                                                                          tAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATgg                                                            >  1:628484/1‑239 (MQ=255)                                                                                                                                                                           aaaCAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGc                                                           <  2:422588/239‑1 (MQ=255)                                                                                                                                                                             aCAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTa                                                        <  1:238264/240‑1 (MQ=255)                                                                                                                                                                                 gCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTgg                                                    >  2:355190/1‑240 (MQ=255)                                                                                                                                                                                           tCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCCAag                                                                                      >  2:88185/1‑196 (MQ=255)                                                                                                                                                                                              tGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAgg                                        >  2:215278/1‑239 (MQ=255)                                                                                                                                                                                               gCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAgga                                       <  1:672186/239‑1 (MQ=255)                                                                                                                                                                                                ccATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAgga                                       <  2:528857/238‑1 (MQ=255)                                                                                                                                                                                                          cacaACATTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATTGCGTACTGGAGTTATTGCAGGATGGATTTGGttt                           >  2:236053/1‑240 (MQ=255)                                                                                                                                                                                                                aTTAAGTTCGAGATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTtcctcc                      <  1:582173/239‑1 (MQ=255)                                                                                                                                                                                                                         gagaTTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCTATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAg             >  2:321982/1‑239 (MQ=255)                                                                                                                                                                                                                          agaTTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGAc           <  1:162007/240‑1 (MQ=255)                                                                                                                                                                                                                                   ccAAGCTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTAc  <  1:717139/240‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           CGTATTAAACATACCTTATTAAGTTTGAATCTTGTAATTTCCAACGCTTCCCGTTTTATCTTAAATGCGAAGTGAACAGATTTCTGGCTCGTCACTCAATCCGTCTTGTCGTTTCAGTTCTGCGTACTCTCCTGTGACCAGGCAGCGAAAAGACATGAGTCGATGACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTAC  >  NC_000913/3966167‑3966632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑