Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,001,379	A→G	T722A (ACC→GCC)	elfC →	putative fimbrial usher protein ElfC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,001,379	0	A	G	100.0%	243.1 / NA	67	T722A (ACC→GCC)	elfC	putative fimbrial usher protein ElfC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/24);  total (43/24)

GCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGCCCATGCGGGTGCAAGGGTATTAATGAAAACATCAAAGCAGGGTATACCGCTGCGTTTTGGCGC  >  NC_000913/1001149‑1001614                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            gCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCt                                                                                                                                                                                                                                      >  1:432238/1‑238 (MQ=255)  cGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGaa                                                                                                                                                                                                                                   >  1:156971/1‑240 (MQ=255)         aaaaCGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAactga                                                                                                                                                                                                                             <  1:131858/239‑1 (MQ=255)                           gcCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCgtt                                                                                                                                                                                                          <  1:451200/240‑1 (MQ=255)                            ccAGCATGTATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCgtt                                                                                                                                                                                                          <  1:190096/239‑1 (MQ=255)                                 aTGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCt                                                                                                                                                                                                    <  1:63753/240‑1 (MQ=255)                                 aTGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCt                                                                                                                                                                                                    <  1:595502/240‑1 (MQ=255)                                     aTTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCGGAAAACTGACTGGCGCGGATAGACCGTTGTTCCTTATg                                                                                                                                                                                                >  1:92241/1‑240 (MQ=255)                                         cAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCAc                                                                                                                                                                                            >  1:654508/1‑240 (MQ=255)                                              gggCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAactgac                                                                                                                                                                                                                            >  1:547975/1‑203 (MQ=255)                                               ggCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGAtata                                                                                                                                                                                                               >  1:193596/1‑215 (MQ=255)                                               ggCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGAtata                                                                                                                                                                                                               <  2:193593/215‑1 (MQ=255)                                               ggCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCActtctt                                                                                                                                                                                       >  1:491361/1‑239 (MQ=255)                                                 cGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTAt                                                                                                                                                                                     >  2:346460/1‑239 (MQ=255)                                                                                     aTAACGGCAGTCAACAACAACTTAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACt                                                                                                                                                                 >  1:387591/1‑223 (MQ=255)                                                                                     aTAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTa                                                                                                                                                 <  2:54199/239‑1 (MQ=255)                                                                                     aTAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTa                                                                                                                                                 <  1:627127/239‑1 (MQ=255)                                                                                      tAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTa                                                                                                                                                 <  2:493219/238‑1 (MQ=255)                                                                                               tCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAaacgtaacgt                                                                                                                                      >  2:559743/1‑240 (MQ=255)                                                                                                           gAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGaaaa                                                                                                                          >  2:439613/1‑240 (MQ=255)                                                                                                             aCTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATg                                                                                                                        <  1:346465/240‑1 (MQ=255)                                                                                                              cTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGc                                                                                                                       <  1:248453/240‑1 (MQ=255)                                                                                                                 cGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCag                                                                                                                     >  1:705136/1‑239 (MQ=255)                                                                                                                      tttCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTc                                                                                                                >  2:209932/1‑239 (MQ=255)                                                                                                                         cAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACg                                                                                                            <  2:114520/240‑1 (MQ=255)                                                                                                                            gCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTgg                                                                                                         <  1:494780/240‑1 (MQ=255)                                                                                                                                tttAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCActtctt                                                                                                                                                                                       >  1:360559/1‑158 (MQ=255)                                                                                                                                tttAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTcc                                                                                                     >  1:662065/1‑240 (MQ=255)                                                                                                                                tttAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTcc                                                                                                     >  1:529366/1‑240 (MQ=255)                                                                                                                                        cccATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCaaa                                                                                              <  2:364797/239‑1 (MQ=255)                                                                                                                                          cATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGGTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTAGAGAGGATATACCCTTGTTTCTTATGCCACTTGTGATCGGGAAaatcga                                                                                                                                                                         >  2:122878/1‑162 (MQ=255)                                                                                                                                            ttCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAgggg                                                                                          >  2:491036/1‑239 (MQ=255)                                                                                                                                              cacaGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTTACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCa                                                                                                                      <  1:688630/209‑1 (MQ=255)                                                                                                                                                caGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGtt                                                                                      <  2:156969/239‑1 (MQ=255)                                                                                                                                                       ttACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGcgtc                                                                                                                                                     >  2:21889/1‑169 (MQ=255)                                                                                                                                                        tACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTgg                                                                              >  1:637717/1‑239 (MQ=255)                                                                                                                                                               gggCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGtt                                                                       >  1:250263/1‑239 (MQ=255)                                                                                                                                                               gggCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGtt                                                                       >  1:545458/1‑239 (MQ=255)                                                                                                                                                               gggCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTc                                                                      >  2:681672/1‑240 (MQ=255)                                                                                                                                                               gggCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGATGGTACTGGCGGAGTTc                                                                      >  1:315909/1‑240 (MQ=255)                                                                                                                                                                    aTCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAggg                                                                                           >  1:442385/1‑214 (MQ=255)                                                                                                                                                                    aTCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAg                                                                                             >  2:579670/1‑212 (MQ=255)                                                                                                                                                                    aTCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGc                                                                 >  2:275187/1‑240 (MQ=255)                                                                                                                                                                    aTCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGc                                                                 >  2:447041/1‑240 (MQ=255)                                                                                                                                                                    aTCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGCTGGt                                                                                   >  1:310889/1‑222 (MQ=255)                                                                                                                                                                    aTCGCTGGGGG‑AACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAAAGTAACGAGGATCTTGAAAATGCAGTAGTGAACg                                                                                                            >  1:63623/1‑196 (MQ=255)                                                                                                                                                                     tCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGcc                                                                >  1:431696/1‑240 (MQ=255)                                                                                                                                                                      cGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTcc                                                                                                     >  2:72197/1‑202 (MQ=255)                                                                                                                                                                          gggggAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGCCCATGc                                                           <  1:559752/240‑1 (MQ=255)                                                                                                                                                                           ggggAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGtt                                                                                                       <  2:728848/195‑1 (MQ=255)                                                                                                                                                                           ggggAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGtt                                                                                                       >  1:728861/1‑195 (MQ=255)                                                                                                                                                                                aaCTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGCCCATGCGGGTGc                                                     <  2:632181/240‑1 (MQ=255)                                                                                                                                                                                 aCTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTTTTCCTTATGCCACTTCTTTACGGGAACAACGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGGGGATCATGAAAATg                                                                                                                        >  2:12713/1‑172 (MQ=255)                                                                                                                                                                                 aCTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGCCCATGCGGGTGc                                                     >  1:243215/1‑239 (MQ=255)                                                                                                                                                                                   tAACGTTCTGATTGCAGCACCAGGCGCAGGGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGCCCATGCGGGTGCaa                                                   <  2:304042/239‑1 (MQ=255)                                                                                                                                                                                                      ccAGGCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGCCCATGCGGGTGCAAGGGTATTAATGAAAACATCa                               <  2:613572/240‑1 (MQ=255)                                                                                                                                                                                                         ggCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGCTATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGCCCATGCGGGTGCAAGGGTATTAATGAAAACATCaaa                             >  2:550273/1‑239 (MQ=255)                                                                                                                                                                                                         ggCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGCCCATGCGGGTGCAAGGGTa                                              >  2:483816/1‑222 (MQ=255)                                                                                                                                                                                                         ggCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGCCCATGCGGGTGCAAGGGTATTAATGAAAACATCAAAg                            <  1:509635/240‑1 (MQ=255)                                                                                                                                                                                                         ggCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGCCCATGCGGGTGCAAGGGTATTAATGAAAACATCAAAg                            >  1:82465/1‑240 (MQ=255)                                                                                                                                                                                                         ggCGCAGAGAATACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGCCCATGCGGGTGCAAGGGTATTAATGAAAACATCAAAg                            <  1:38828/240‑1 (MQ=255)                                                                                                                                                                                                                    tACTCGTGTGGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGCCCATGCGGGTGCAAGGGTATTAATGAAAACATCAAAGCAGGGTATAcc                 >  1:31776/1‑240 (MQ=255)                                                                                                                                                                                                                          tgtgGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATgc                                                                                                                                                           >  2:707091/1‑96 (MQ=255)                                                                                                                                                                                                                          tgtgGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAgggg                                                                                          >  2:190462/1‑161 (MQ=255)                                                                                                                                                                                                                          tgtgGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGCCCATGCGGGTGCAAGGGTATTAATGAAAACATCAAAGCAGGGTATACCGCTGc            >  1:90153/1‑239 (MQ=255)                                                                                                                                                                                                                            tgGCGAACAGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGCCCATGCGGGTGCAAGGGTATTAATGAAAACATCAAAGCAGGGTATACCGCTGCGtt         >  2:687100/1‑240 (MQ=255)                                                                                                                                                                                                                                    aGCGCCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGCCCATGCGGGTGCAAGGGTATTAATGAAAACATCAAAGCAGGGTATACCGCTGCGTTTTGgcgc  <  1:307468/239‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            GCGAGGGGAAAACGGCTAATGGTAGCGCCAGCATGGATTACAAAGGGGCGTTTGCGGATGCCCGAGTGGGCTACAACTACAGCGATAACGGCAGTCAACAACAACTGAACTACGCTCTTTCAGGCAGTTTAGTTGCCCATTCACAGGGCATTACCCTGGGGCAATCGCTGGGGGAAACTAACGTTCTGATTGCAGCACCAGGCGCAGAGAATACTCGTGTGGCGAACAGCACCGGGCTGAAAACTGACTGGCGCGGATATACCGTTGTTCCTTATGCCACTTCTTATCGGGAAAATCGAATCGCACTTGATGCGGCGTCGTTAAAACGTAACGTGGATCTTGAAAATGCAGTAGTCAACGTGGTTCCCACCAAAGGGGCGTTGGTTCTGGCGGAGTTCAATGCCCATGCGGGTGCAAGGGTATTAATGAAAACATCAAAGCAGGGTATACCGCTGCGTTTTGGCGC  >  NC_000913/1001149‑1001614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑