Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,165,718	A→G	intergenic (+33/‑367)	ycfP → / → ndh	UPF0227 protein YcfP/NADH:quinone oxidoreductase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,165,718	0	A	G	100.0%	235.1 / NA	65	intergenic (+33/‑367)	ycfP/ndh	UPF0227 protein YcfP/NADH:quinone oxidoreductase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/42);  total (23/42)

AAGTGTGTGACCAACTTCCGTGAGAAGAATCGCGATCGTTGCCTGGTGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTT  >  NC_000913/1165482‑1165952                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                           aaGTGTGTGACCAACTTCCGTGAGAAGAATCGCGATCGTTGCCTGGTGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGcc                                                                                                                                                                                                                                          >  2:464523/1‑239 (MQ=255)      tgtgACCAACTTCCGTGAGAAGAATCGCGATCGTTGCCTGGTGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATgc                                                                                                                                                                                                                                    >  1:463699/1‑240 (MQ=255)           ccAACTTCCGTGAGAAGAATCGCGATCGTTGCCTGATGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTcc                                                                                                                                                                                                                               <  2:181901/240‑1 (MQ=255)              aCTTCCGTGAGAAGAATCGCGATCGTTGCCTGGTGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGcc                                                                                                                                                                                                                                          >  2:109294/1‑226 (MQ=255)              aCTTCCGTGAGAAGAATCGCGATCGTTGCCTGGTGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGAt                                                                                                                                                                                                                                      >  2:254044/1‑230 (MQ=255)              aCTTCCGTGAGAAGAATCGCGATCGTTGCCTGGTGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGaa                                                                                                                                                                                                                            >  1:708748/1‑240 (MQ=255)              aCTTCCGTGAGAAGAATCGCGATCGTTGCCTGGTGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGaa                                                                                                                                                                                                                            >  1:370069/1‑240 (MQ=255)              aCTTCCGTGAGAAGAATCGCGATCGTTGCCTGGTGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGaa                                                                                                                                                                                                                            >  2:506347/1‑240 (MQ=255)                        gaagaaTCGCGATCGTTGCCTGGTGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGt                                                                                                                                                                                                                  <  1:624386/240‑1 (MQ=255)                         aagaaTCGCGATTGTTGCCTGGTGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGt                                                                                                                                                                                                                  <  1:379709/239‑1 (MQ=255)                         aagaaTCGCGATCGTTGCCTGGTGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGt                                                                                                                                                                                                                  <  2:533885/239‑1 (MQ=255)                             aTCGCGATCGTTGCCTGGTGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGa                                                                                                                                                                                                              >  2:238402/1‑239 (MQ=255)                                       ttGCCTGGTGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTcc                                                                                                                                                                                                    <  1:172321/239‑1 (MQ=255)                                         gCCTGGTGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCAcg                                                                                                                                                                                                 <  1:676199/240‑1 (MQ=255)                                              gTGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCa                                                                                                                                                                                            <  2:561038/240‑1 (MQ=255)                                                         gcgTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTgg                                                                                                                                                                                                                    <  2:450640/205‑1 (MQ=255)                                                           gTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGa                                                                                                                                                                                <  2:249626/239‑1 (MQ=255)                                                                          ttAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATg                                                                                                                                                                <  2:444931/240‑1 (MQ=255)                                                                                gccagcGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCt                                                                                                                                                                    <  2:253951/230‑1 (MQ=255)                                                                                  cagcGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGcttctt                                                                                                                                                        <  1:681786/240‑1 (MQ=255)                                                                                                agagTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGa                                                                                                                                          <  1:37561/240‑1 (MQ=255)                                                                                                    ttGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTAt                                                                                                                                                      <  1:522721/224‑1 (MQ=255)                                                                                                    ttGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACg                                                                                                                                       >  1:127208/1‑239 (MQ=255)                                                                                                    ttGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGc                                                                                                                                      <  1:576085/240‑1 (MQ=255)                                                                                                    ttGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGc                                                                                                                                      >  1:38185/1‑240 (MQ=255)                                                                                                      gCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCt                                                                                                                                                                    >  1:265050/1‑208 (MQ=255)                                                                                                      gCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGccc                                                                                                                                    <  1:159246/240‑1 (MQ=255)                                                                                                      gCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGcc                                                                                                                                     <  2:96693/239‑1 (MQ=255)                                                                                                       catcatTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGccc                                                                                                                                    <  2:103365/239‑1 (MQ=255)                                                                                                       catcatTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCt                                                                                                                                   <  1:464531/240‑1 (MQ=255)                                                                                                          catTATTACGGGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGccc                                                                                                                                    <  1:541190/236‑1 (MQ=255)                                                                                                           attattACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCAt                                                                                                                               <  2:154448/240‑1 (MQ=255)                                                                                                             tattaCGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATac                                                                                                                             <  2:685617/240‑1 (MQ=255)                                                                                                                   gagaTTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCt                                                                                                                       <  1:587846/240‑1 (MQ=255)                                                                                                                   gagaTTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCt                                                                                                                       <  2:370064/240‑1 (MQ=255)                                                                                                                   gagaTTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCt                                                                                                                       <  2:353128/240‑1 (MQ=255)                                                                                                                   gagaTTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCCGCATACACCCCt                                                                                                                       <  2:593631/240‑1 (MQ=255)                                                                                                                    agaTTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCt                                                                                                                       <  1:625532/239‑1 (MQ=255)                                                                                                                    agaTTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCt                                                                                                                       <  2:16663/239‑1 (MQ=255)                                                                                                                    agaTTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCt                                                                                                                       <  1:31058/239‑1 (MQ=255)                                                                                                                       ttGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCAct                                                                                                                   <  1:238405/240‑1 (MQ=255)                                                                                                                       ttGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCAct                                                                                                                   <  1:33042/240‑1 (MQ=255)                                                                                                                        tGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATacg                                                                                                                                                                                                          >  2:504598/1‑150 (MQ=255)                                                                                                                                    agagCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATc                                                                                                                                                                           >  1:679477/1‑171 (MQ=255)                                                                                                                                    agagCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATc                                                                                                                                                                           <  2:679465/171‑1 (MQ=255)                                                                                                                                    agagCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCActct                                                                                                       >  2:38490/1‑239 (MQ=255)                                                                                                                                    agagCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACtctc                                                                                                      >  1:287146/1‑240 (MQ=255)                                                                                                                                                caAAATCAAGAATATCTCCCCGCATTTACCGCGCATTAAAGCGTTCAATGCCCTCGGGTAAATGCCCTCGTCGCAACAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAATGCGTCCAcgccg                                                                                                                                                                                              <  2:422663/140‑1 (MQ=255)                                                                                                                                                 aaaTTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCTCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCCCGCCGCATCCGACAGTCGAGCATc                                                                                                                                                                           <  2:460068/158‑1 (MQ=255)                                                                                                                                                  aaTTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATac                                                                                                                             <  1:715527/203‑1 (MQ=255)                                                                                                                                                          aaTATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAAtaataa                                                                                 <  1:316783/239‑1 (MQ=255)                                                                                                                                                                          tACAGCGCATTAGAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATaca                                                                                                                            <  1:439554/180‑1 (MQ=255)                                                                                                                                                                                 cATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACAccc                                                                                                                         >  1:458012/1‑176 (MQ=255)                                                                                                                                                                                   ttAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGAtctc                                                        <  2:46962/239‑1 (MQ=255)                                                                                                                                                                                           ttCAAAACCCTCGGGTAAATTCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATaataaacaataaac                                                                        <  1:416956/215‑1 (MQ=255)                                                                                                                                                                                           ttCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTaa                                               >  1:277104/1‑240 (MQ=255)                                                                                                                                                                                                  cccTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCa                                                                                                                     <  1:77027/163‑1 (MQ=255)                                                                                                                                                                                                        ggTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCtt                                  >  2:661497/1‑240 (MQ=255)                                                                                                                                                                                                                   tcgtcgCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGcaacaa                       >  1:296300/1‑240 (MQ=255)                                                                                                                                                                                                                   tcgtcgCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGcaacaa                       >  2:668823/1‑240 (MQ=255)                                                                                                                                                                                                                   tcgtcgCATCAGGTAACCTTGCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGcaacaa                       >  2:204793/1‑240 (MQ=255)                                                                                                                                                                                                                                       gCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACTCGGTAAAGTCGCCTATCTTTTCAGca                           >  1:505786/1‑216 (MQ=255)                                                                                                                                                                                                                                       gCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAAtt    <  2:58589/239‑1 (MQ=255)                                                                                                                                                                                                                                       gCCGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAAtt    <  1:129676/239‑1 (MQ=255)                                                                                                                                                                                                                                        ccGGTGCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAAtttt  >  1:496465/1‑240 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                           AAGTGTGTGACCAACTTCCGTGAGAAGAATCGCGATCGTTGCCTGGTGATTTTGTCGCGTAATGATGAAGCGCTTAACAGCCAGCGGACATCTGAAGAGTTGCATCATTATTACGAGATTGTCTGGGACGAAGAGCAGACGCACAAATTCAAGAATATCTCCCCGCATTTACAGCGCATTAAAGCGTTCAAAACCCTCGGGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTT  >  NC_000913/1165482‑1165952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑