Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,953,402	A→G	N234S (AAC→AGC)	cmoA →	carboxy‑S‑adenosyl‑L‑methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,953,402	0	A	G	96.8%	104.6 / ‑6.0	31	N234S (AAC→AGC)	cmoA	carboxy‑S‑adenosyl‑L‑methionine synthase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (12/18);  total (12/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GCGCGCTGGTGCTTTCGGAAAAATTCAGTTTCGAAGATGCCAAAGTTGGTGAACTGTTGTTCAACATGCACCACGACTTTAAACGTGCCAACGGTTACAGCGAACTGGAGATCAGCCAGAAGCGCAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCAT  >  NC_000913/1953174‑1953624                                                                                                                                                                                                                                     |                                                                                                                                                                                                                               gcgcgcTGGTGCTTTCGGAAAAATTCAGTTTCGAAGATGCCAAAGTTGGTGAACTGTTGTTCAACATGCACCACGACTTTAAACGTGCCAACGGTTACAGCGAACTGGAGATCAGCCAGAAGCGCAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCAc                                                                                                                                                                                                                     >  1:300015/1‑240 (MQ=255)       tGGTGCTTTCGGAAAAATTCAGTTTCGAAGATGCCAAAGTTGGTGAACTGTTGTTCAACATGCACCACGACTTTAAACGTGCCAACGGTTACAGCGAACTGGAGATCAGCCAGAAGCGCAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACtggtg                                                                                                                                                                                                                <  1:144152/239‑1 (MQ=255)             tttCGGAAAAATTCAGTTTCGAAGATGCCAAAGTTGGTGAACTGTTGTTCAACATGCACCACGACTTTAAACGTGCCAACGGTTACAGCGAACTGGAGATCAGCCAGAAGCGCAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTaa                                                                                                                                                                                                         <  1:595671/240‑1 (MQ=255)                       aTTCAGTTTCGAAGATGCCAAAGTTGGTGAACTGTTGTTCAACATGCACCACGACTTTAAACGTGCCAACGGTTACAGCGAACTGGAGATCAGCCAGAAGCGCAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGa                                                                                                                                                                                               <  1:601148/240‑1 (MQ=255)                           aGTTTCGAAGATGCCAAAGTTGGTGAACTGTTGTTCAACATGCACCACGACTTTAAACGTGCCAACGGTTACAGCGAACTGGAGATCAGCCAGAAGCGCAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACtggt                                                                                                                                                                                                                 <  2:412657/218‑1 (MQ=255)                                                         ttgttCAACATGCACCACGACTTTAAACGTGCCAACGGTTACAGCGAACTGGAGATCAGCCAGAAGCGCAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGa                                                                                                                                                                                               <  2:457734/206‑1 (MQ=255)                                                                          cGACTTTAAACGTGCCAACGGTTACAGCGAACTGGAGATCAGCCAGAAGCGCAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAatcat                                                                                                                                            >  1:491187/1‑240 (MQ=255)                                                                           gACTTTAAACGTGCCAACGGTTACAGCGAACTGGAGATCAGCCAGAAGCGCAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAatcat                                                                                                                                            >  1:49386/1‑239 (MQ=255)                                                                                  aaCGTGCCAACGGTTACAGCGAACTGGAGATCAGCCAGAAGCGCAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca                                                                                                                                    <  1:373669/240‑1 (MQ=255)                                                                                  aaCGTGCCAACGGTTACAGCGAACTGGAGATCAGCCAGAAGCGCAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTcaca                                                                                                                                    <  1:45656/240‑1 (MQ=255)                                                                                               ttACAGCGAACTGGAGATCAGCCAGAAGCGCAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACg                                                                                                                       >  1:7641/1‑240 (MQ=255)                                                                                                           ggAGATCAGCCAGAAGCGCAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCa                                                                                                            <  1:482739/239‑1 (MQ=255)                                                                                                             agaTCAGCCAGAAGCGCAGCATGCTGGAAAACGTGATGCTCACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCTGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAatc                                                                                                                                              <  2:137675/203‑1 (MQ=255)                                                                                                                         aGCGCAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCa                                                                                             <  1:276535/240‑1 (MQ=255)                                                                                                                           cgcAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAgc                                                                                           >  1:690308/1‑240 (MQ=255)                                                                                                                                        aaaaCGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAgcagcagca                                                                              <  2:491178/240‑1 (MQ=255)                                                                                                                                           aCGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACgg                                                                           <  1:29216/240‑1 (MQ=255)                                                                                                                                            cGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACggg                                                                          >  1:364534/1‑240 (MQ=255)                                                                                                                                               gATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAatc                                                                                                                                              <  2:206826/169‑1 (MQ=255)                                                                                                                                                      aCCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGc                                                                >  1:110514/1‑240 (MQ=255)                                                                                                                                                       ccGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCa                                                               <  1:90812/240‑1 (MQ=255)                                                                                                                                                         gATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCCGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGcgcg                                                                                        <  1:354878/213‑1 (MQ=255)                                                                                                                                                                tGGAAACCCATAAAGCACGCCTGCATAACGCCGGGTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCaca                                                                                                                                                  >  1:276292/1‑146 (MQ=255)                                                                                                                                                                                 cGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGTTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTc                                        >  2:580655/1‑237 (MQ=255)                                                                                                                                                                                 cGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTCTAc                                     >  1:45710/1‑240 (MQ=255)                                                                                                                                                                                 cGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTCTAc                                     >  2:702274/1‑240 (MQ=255)                                                                                                                                                                                              ccGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTCTAcc                                    <  2:432026/228‑1 (MQ=255)                                                                                                                                                                                                          aTAGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTg             <  1:76703/239‑1 (MQ=255)                                                                                                                                                                                                            aGTGAGCTGTGGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGAtt          >  2:417851/1‑240 (MQ=255)                                                                                                                                                                                                                   tgtgGTTCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACgg                                                                           <  2:570020/168‑1 (MQ=255)                                                                                                                                                                                                                    gtgGTTCCAGTGCTTTAGCTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCAt  <  1:569700/240‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                               GCGCGCTGGTGCTTTCGGAAAAATTCAGTTTCGAAGATGCCAAAGTTGGTGAACTGTTGTTCAACATGCACCACGACTTTAAACGTGCCAACGGTTACAGCGAACTGGAGATCAGCCAGAAGCGCAGCATGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAGCAGTGGTCAAATGCGGTGGAGTTTCTACCTGAAATTAAACCGTATCGTCTTGATTTATTGCAT  >  NC_000913/1953174‑1953624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑