Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,173,142	T→C	pseudogene (1137/1358 nt)	gatC ←	galactitol‑specific PTS enzyme IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,173,142	0	T	C	100.0%	126.5 / NA	35	pseudogene (1137/1358 nt)	gatC	galactitol‑specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/19);  total (16/19)

CCCCTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCC  >  NC_000913/2172906‑2173375                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                          ccccTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCGGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGcgtg                                                                                                                                                                                                                                        <  1:326236/240‑1 (MQ=255) ccccTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGcgtg                                                                                                                                                                                                                                        <  1:181039/240‑1 (MQ=255) ccccTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGcgtg                                                                                                                                                                                                                                        <  2:603302/240‑1 (MQ=255)  cccTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGc                                                                                                                                                                                                                                       >  1:323026/1‑240 (MQ=255)   ccTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCa                                                                                                                                                                                                                                      <  1:627126/240‑1 (MQ=255)                          agaACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGAt                                                                                                                                                                                                                <  1:210790/239‑1 (MQ=255)                              cGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCcaca                                                                                                                                                                                                           <  2:208028/240‑1 (MQ=255)                                   ttCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTg                                                                                                                                                                                                      <  1:142417/240‑1 (MQ=255)                                         tGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTc                                                                                                                                                                                                <  1:540231/240‑1 (MQ=255)                                                  aaGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTg                                                                                                                                                                                                      >  2:254656/1‑225 (MQ=255)                                                        cgcgCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACAcc                                                                                                                                                                                  <  2:424699/239‑1 (MQ=255)                                                                     aGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACAcc                                                                                                                                                                                  <  2:577700/226‑1 (MQ=255)                                                                       gTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCa                                                                                                                                                                                                                                      >  1:352778/1‑172 (MQ=255)                                                                       gTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCa                                                                                                                                                                                                                                      <  2:352773/172‑1 (MQ=255)                                                                       gTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTcgc                                                                                                                                                                                                                   <  1:365050/191‑1 (MQ=255)                                                                       gTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTcgc                                                                                                                                                                                                                   >  2:365045/1‑191 (MQ=255)                                                                       gTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCgg                                                                                                                                                                  >  1:110574/1‑240 (MQ=255)                                                                           tGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAAc                                                                                                                                                               >  1:383663/1‑239 (MQ=255)                                                                           tGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACa                                                                                                                                                              >  1:229608/1‑240 (MQ=255)                                                                           tGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACa                                                                                                                                                              >  1:146453/1‑240 (MQ=255)                                                                           tGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACa                                                                                                                                                              >  1:374285/1‑240 (MQ=255)                                                                                                 tGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGAc                                                                                                                                        <  1:331080/240‑1 (MQ=255)                                                                                                       gATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCa                                                                                                                                   <  2:254423/239‑1 (MQ=255)                                                                                                             aatGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCAc                                                                                                                            >  1:185223/1‑240 (MQ=255)                                                                                                               tGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGAt                                                                                                                                                                                                    <  2:542969/166‑1 (MQ=255)                                                                                                                                    gggAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGAt                                                                                                     <  2:287756/240‑1 (MQ=255)                                                                                                                                              cTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACgg                                                                                           <  2:345953/240‑1 (MQ=255)                                                                                                                                              cNGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTg                                                                                                                                                                     <  2:504963/166‑1 (MQ=255)                                                                                                                                                 aaTCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgca                                                                                         >  2:43016/1‑239 (MQ=255)                                                                                                                                                    cAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGgcagca                                                                                      <  1:396505/239‑1 (MQ=255)                                                                                                                                                              gTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTACGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGaggctg                                                                                                            >  2:575402/1‑202 (MQ=255)                                                                                                                                                                        aaCCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACaaa                                                                 <  2:532820/240‑1 (MQ=255)                                                                                                                                                                                  aTCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTaa                                                       >  2:727427/1‑240 (MQ=255)                                                                                                                                                                                            gCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAAc                                                                                             >  2:33873/1‑192 (MQ=255)                                                                                                                                                                                                 caTACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAt                                                    >  1:509299/1‑228 (MQ=255)                                                                                                                                                                                                  aTACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATa                                       <  1:420831/240‑1 (MQ=255)                                                                                                                                                                                                                                gccgccAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccg           <  1:285737/238‑5 (MQ=255)                                                                                                                                                                                                                                     cAGTTGGGCGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTcc  >  1:592683/1‑240 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                          CCCCTCAGGTAAAAATTATTCTGCGAGAACGACTTTCTCTTGTTTAATAAAGCCACGCGCTCTACGCCAGGTCATGAAAATACCGGTCAGATAAATTGCACCGATAATAATGAAACCGGGAATATTTTGCGGGGAGAAAACCTGAATCAGTAACCAGGTAATGGGAGAACCGCCCTGATCCATTGAAGCCACCATACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCC  >  NC_000913/2172906‑2173375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑