Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,406,477	T→C	K55K (AAA→AAG)	lrhA ←	DNA‑binding transcriptional dual regulator LrhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,406,477	0	T	C	100.0%	296.7 / NA	80	K55K (AAA→AAG)	lrhA	DNA‑binding transcriptional dual regulator LrhA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/52);  total (28/52)

GACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCA  >  NC_000913/2406246‑2406703                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                   gACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAAc                                                                                                                                                                                                                            >  1:562234/1‑240 (MQ=255)    aTCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAg                                                                                                                                                                                                                         <  1:378950/240‑1 (MQ=255)                                     gcgATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTAcgg                                                                                                                                                                                         >  1:725234/1‑239 (MQ=255)                                        aTTTAACAGGAAGGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGa                                                                                                                                                                                     <  1:136555/240‑1 (MQ=255)                                        aTTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGa                                                                                                                                                                                     <  2:577181/240‑1 (MQ=255)                                          ttAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACt                                                                                                                                                                                   <  1:616699/240‑1 (MQ=255)                                            aaCAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGa                                                                                                                                                                                 <  2:383845/240‑1 (MQ=255)                                                      gTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACCCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCt                                                                                                                                                                                                  <  2:103687/213‑1 (MQ=255)                                                           aTCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCg                                                                                                                                                                  >  2:665062/1‑240 (MQ=255)                                                              gTAGCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGAc                                                                                                                                                                                               <  1:689564/208‑1 (MQ=255)                                                               tATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCa                                                                                                                                                               >  1:487693/1‑239 (MQ=255)                                                                aTCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGCGCGAACAGTTCCTTCCCAACGAgttgt                                                                                                                                                                                                                     >  1:436556/1‑184 (MQ=255)                                                                aTCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACCCACAGCGGCAGc                                                                                                                                                             >  2:604686/1‑240 (MQ=255)                                                                 tCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGa                                                                                                                                                                                     <  2:374675/215‑1 (MQ=255)                                                                 tCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGa                                                                                                                                                                                     >  1:374680/1‑215 (MQ=255)                                                                  cGGCAGGTTCATCTGTAGCGCCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCACGCCTCATCATTAAAACGCTGGATTTTCCTGGCGTATCCAAGAAGTTGAATGCCATGTTCATTTAACAGTTTGTTGCGCCGGTGACGAGCGAACAGTTCCTTTCCAACGAGTTTTTCCAGACTTTGCATTTGCTGACTTACGGCGGa                                                                                                                                                                                     <  2:303662/214‑1 (MQ=255)                                                                     cAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTAcg                                                                                                                                                                                          >  2:142602/1‑206 (MQ=255)                                                                       gATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGGAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGAc                                                                                                                                                                                               <  2:377322/199‑1 (MQ=255)                                                                          tCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGgcagc                                                                                                                                                    >  1:253629/1‑239 (MQ=255)                                                                            aTCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAATGAg                                                                                                                                                                                                                         <  2:487072/168‑1 (MQ=255)                                                                            aTCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGgcagca                                                                                                                                                   <  2:726493/238‑1 (MQ=255)                                                                             tCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGc                                                                                                                                                          <  2:380501/230‑1 (MQ=255)                                                                             tCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCaaaa                                                                                                                                                >  2:21220/1‑240 (MQ=255)                                                                             tCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCaaaa                                                                                                                                                >  1:191869/1‑240 (MQ=255)                                                                                              tAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGgcaac                                                                                                                               <  1:677054/240‑1 (MQ=255)                                                                                               aaTACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGGGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGgcaac                                                                                                                               <  1:115366/239‑1 (MQ=255)                                                                                               aaTACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGgcaaca                                                                                                                              >  1:436017/1‑240 (MQ=255)                                                                                                        tGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACaaa                                                                                                                      >  2:105031/1‑239 (MQ=255)                                                                                                         gAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATg                                                                                                                    >  1:137211/1‑240 (MQ=255)                                                                                                                aCTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTcag                                                                                                             <  2:253626/240‑1 (MQ=255)                                                                                                                      cATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGc                                                                                                                                                                >  1:350729/1‑183 (MQ=255)                                                                                                                      cATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGAtc                                                                                                       <  1:507591/240‑1 (MQ=255)                                                                                                                         tAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGcaacaa                                                                                                                       <  2:555482/221‑1 (MQ=255)                                                                                                                            tgatgaGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGcaacaa                                                                                                                       <  2:425708/218‑1 (MQ=255)                                                                                                                            tgatgaGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGAtcgaggtc                                                                                                 <  1:331361/240‑1 (MQ=255)                                                                                                                            tgatgaGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGAtcgaggt                                                                                                  <  1:669148/239‑1 (MQ=255)                                                                                                                             gatgaGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGAtcgaggtcg                                                                                                <  1:658821/240‑1 (MQ=255)                                                                                                                              atgaGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGCGTACGac                                                                                                                                                                          >  2:190840/1‑165 (MQ=255)                                                                                                                                  gCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATcgaggtcgaggt                                                                                            <  1:565389/239‑1 (MQ=255)                                                                                                                                   cAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTa                                                                                          >  1:413362/1‑240 (MQ=255)                                                                                                                                        cTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGAtcgagg                                                                                                   >  1:384842/1‑226 (MQ=255)                                                                                                                                        cTCATCATAAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGGTGCGACCGTGACGAGCGAACTGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGATTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTa                                                                                      >  2:305357/1‑239 (MQ=255)                                                                                                                                           atcatTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCgg                                                                                  >  2:75251/1‑240 (MQ=255)                                                                                                                                           atcatTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCgg                                                                                  >  1:696095/1‑240 (MQ=255)                                                                                                                                           atcatTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCgg                                                                                  <  1:694638/240‑1 (MQ=255)                                                                                                                                                     cGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGAttt                                                                           >  1:417637/1‑237 (MQ=255)                                                                                                                                                     cGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCa                                                                        <  1:260205/240‑1 (MQ=255)                                                                                                                                                      gCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCa                                                                        <  1:416102/239‑1 (MQ=255)                                                                                                                                                      gCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCAc                                                                       <  1:28965/240‑1 (MQ=255)                                                                                                                                                       cAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCAc                                                                       <  2:441383/239‑1 (MQ=255)                                                                                                                                                            ttttCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCa                                                                 <  1:618442/240‑1 (MQ=255)                                                                                                                                                            ttttCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCa                                                                 <  1:578361/240‑1 (MQ=255)                                                                                                                                                                ccTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCatata                                                             <  1:21220/240‑1 (MQ=255)                                                                                                                                                                       tAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCAc                                                                       <  2:78707/223‑1 (MQ=255)                                                                                                                                                                           cAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGa                                                                                 <  2:188782/209‑1 (MQ=255)                                                                                                                                                                             agaagTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTg                                                <  2:254118/240‑1 (MQ=255)                                                                                                                                                                                  ttGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACtt                                                                     <  1:732808/214‑1 (MQ=255)                                                                                                                                                                                  ttGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTggcggc                                           <  2:181086/240‑1 (MQ=255)                                                                                                                                                                                   tGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACGCAGCGGCAGCTGCGGCAGCAAAAGTGTTCTGATCGGCAACAGCAACAAATTTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTggc                                              <  2:305693/236‑1 (MQ=255)                                                                                                                                                                                   tGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTggcggc                                           <  2:353934/239‑1 (MQ=255)                                                                                                                                                                                    gAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGac                                                                                                                                                                          >  2:430769/1‑111 (MQ=255)                                                                                                                                                                                    gAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTAt                                                                   >  2:629439/1‑214 (MQ=255)                                                                                                                                                                                       tGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATa                                      <  1:466803/240‑1 (MQ=255)                                                                                                                                                                                            tGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAg                                 <  1:10527/240‑1 (MQ=255)                                                                                                                                                                                            tGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAg                                 <  1:124642/240‑1 (MQ=255)                                                                                                                                                                                            tGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAg                                 <  1:279742/240‑1 (MQ=255)                                                                                                                                                                                            tGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAg                                 >  2:578279/1‑240 (MQ=255)                                                                                                                                                                                            tGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAg                                 <  2:185865/240‑1 (MQ=255)                                                                                                                                                                                             gTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAg                                 <  1:724932/239‑1 (MQ=255)                                                                                                                                                                                                  gTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAGCTGGt                            <  2:417630/239‑1 (MQ=255)                                                                                                                                                                                                   ttAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGac                                                                                                                                                                          >  1:93077/1‑96 (MQ=255)                                                                                                                                                                                                      aCAGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATg                       <  2:62933/240‑1 (MQ=255)                                                                                                                                                                                                        aGTTTGTTGCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCa                                        >  1:534564/1‑221 (MQ=255)                                                                                                                                                                                                           ttgttgCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCtgtg                  <  1:563964/240‑1 (MQ=255)                                                                                                                                                                                                           ttgttgCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCtgtg                  <  1:467472/240‑1 (MQ=255)                                                                                                                                                                                                               tgCGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGcaca              >  2:577034/1‑240 (MQ=255)                                                                                                                                                                                                                 cGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTg                                                <  2:177886/204‑1 (MQ=255)                                                                                                                                                                                                                 cGACCGTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAg                                 <  2:110861/219‑1 (MQ=255)                                                                                                                                                                                                                      gTGACGAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACacaaa        <  1:92505/239‑1 (MQ=255)                                                                                                                                                                                                                           gAGCGAACAGTTCCTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCa  <  2:390229/240‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                   GACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGACTGAGTACGACACACAGCGGCAGCTGCGGCAGCAAAAGTGTTCAGATCGGCAACAGCAACAAATGTTCTCAGCAGATCGAGGTCGAGGTTAATTATCGGACGATTTGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCA  >  NC_000913/2406246‑2406703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑