Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,554,617	G→A	A189V (GCG→GTG)	eutR ←	putative AraC‑type transcriptional regulator EutR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,554,617	0	G	A	100.0%	189.6 / NA	51	A189V (GCG→GTG)	eutR	putative AraC‑type transcriptional regulator EutR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/35);  total (16/35)

TGATTACACAAATCCAGCACCGTCACCGGTTCGGACATGTTTTCCAGCACATATTCACGGGCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGCACCACGCCCAGGATCGTGTAATCATCCGGCGTGCTCAGTTCAAATTCAGTGCCACCAGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGA  >  NC_000913/2554389‑2554849                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         tGATTACACAAATCCAGCACCGTCACCGGTTCGGACATGTTTTCCAGCACATATTCACGGGCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACa                                                                                                                                                                                                                               <  2:96463/240‑1 (MQ=255)  gATTACACAAATCCAGCACCGTCACCGGTTCGGACATGTTTTCCAGCACATATTCACGGGCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACa                                                                                                                                                                                                                               <  1:732736/239‑1 (MQ=255)              ccAGCACCGTCACCGGTTCGGACATGTTTTCCAGCACATATTCACGGGCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAgcg                                                                                                                                                                                                                  <  2:599452/240‑1 (MQ=255)              ccAGCACCGTCACCGGTTCGGACATGTTTTCCAGCACATATTCACGGGCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAgcg                                                                                                                                                                                                                  <  1:613120/240‑1 (MQ=255)                  cACCGTCACCGGTTCGGACATGTTTTCCAGCACATATTCACGGGCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGc                                                                                                                                                                                                              >  1:294732/1‑240 (MQ=255)                      gTCACCGGTTCGGACATGTTTTCCAGCACATATTCACGGGCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGCTGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGc                                                                                                                                                                                                                           >  1:136932/1‑223 (MQ=255)                       tCACCGGTTCGGACATGTTTTCCAGCACATATTCACGGGCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTAt                                                                                                                                                                                                         >  1:660253/1‑240 (MQ=255)                       tCACCGGTTCGGACATGTTTTCCAGCACATATTCACGGGCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTAt                                                                                                                                                                                                         >  1:50705/1‑240 (MQ=255)                                      tGTTTTCCAGCACATATTCACGGGCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAgcg                                                                                                                                                                                                                  <  2:603015/216‑1 (MQ=255)                                        ttttCCAGCACATATTCACGGGCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTcc                                                                                                                                                                                        >  1:671973/1‑240 (MQ=255)                                                   atatTCACGGGCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTg                                                                                                                                                                             <  1:428286/240‑1 (MQ=255)                                                     atTCACGGGCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGt                                                                                                                                                                           >  1:1304/1‑240 (MQ=255)                                                       tCACGGGCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCCAAACGTCGCCAGCACCTg                                                                                                                                                                                                                                      <  2:488022/179‑1 (MQ=255)                                                        cACGGGCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTa                                                                                                                                                                         <  1:561057/239‑1 (MQ=255)                                                            ggCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCGGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGa                                                                                                                                                                                <  1:453430/228‑1 (MQ=255)                                                             gcgcGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCTGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAAc                                                                                                                                                                   <  2:156072/240‑1 (MQ=255)                                                               gcgGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACat                                                                                                                                                                 <  1:37389/240‑1 (MQ=255)                                                               gcgGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACa                                                                                                                                                                  <  1:162796/239‑1 (MQ=255)                                                                cgGGAAAGCACTCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAAc                                                                                                                                                                   <  2:645968/237‑1 (MQ=255)                                                                cgGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTCTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACat                                                                                                                                                                 >  1:187573/1‑239 (MQ=255)                                                                    aaaGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGt                                                                                                                                                             <  2:539701/239‑1 (MQ=255)                                                                       gCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATa                                                                                                                                                         <  2:512935/240‑1 (MQ=255)                                                                                         gatgaCTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTa                                                                                                                                                                                                          >  1:140071/1‑173 (MQ=255)                                                                                                gATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGtt                                                                                                                                >  2:613409/1‑240 (MQ=255)                                                                                                gATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCAAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGtt                                                                                                                                >  1:161424/1‑240 (MQ=255)                                                                                                 aTGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTa                                                                                                                               >  1:69565/1‑240 (MQ=255)                                                                                                         cgccgTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTAc                                                                                                                                                                        <  1:326582/191‑1 (MQ=255)                                                                                                            cgTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCgg                                                                                                                      <  1:328359/238‑1 (MQ=255)                                                                                                                 ccATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGtgatga                                                                                                               <  1:491380/240‑1 (MQ=255)                                                                                                                    ttGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGAc                                                                                                              <  1:358071/238‑1 (MQ=255)                                                                                                                              ttcttcCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACa                                                                                                                                                                                                                               <  2:457342/115‑1 (MQ=255)                                                                                                                                   ccAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGcacc                                                                                              <  1:485071/239‑1 (MQ=255)                                                                                                                                    cAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGcacc                                                                                              <  1:389295/238‑1 (MQ=255)                                                                                                                                     aGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGcacc                                                                                              <  2:77202/237‑1 (MQ=255)                                                                                                                                         tGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGcacc                                                                                              <  2:556554/233‑1 (MQ=255)                                                                                                                                            cccccATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGCACCACGCCCAGGa                                                                                    <  2:505913/240‑1 (MQ=255)                                                                                                                                                 aTCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGCACCACGCCCAGGATCgtg                                                                               >  1:108781/1‑240 (MQ=255)                                                                                                                                                   cGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGCACCACGCCCAGGATCGTGTa                                                                             <  1:420545/240‑1 (MQ=255)                                                                                                                                                    gCCATTAGCAAATTATCCCCCAGCACTTTGCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGa                                                                                                                                                                                                                                 <  2:192154/91‑1 (MQ=255)                                                                                                                                                                 tATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGCACCACGCCCAGGATCGTGTAATCATCCGGCGTGc                                                               <  2:294729/240‑1 (MQ=255)                                                                                                                                                                     ccccAGCACTTGTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGCACCACGCCCAGGATCGTGTAatcat                                                                        <  2:444810/227‑1 (MQ=255)                                                                                                                                                                                ttCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGCACCACGCCCAGGATCGTGTAATCATCCGGCGTGCTCAGTTCAAATTCa                                                 >  2:625997/1‑239 (MQ=255)                                                                                                                                                                                  cGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGAc                                                                                                              <  1:422727/176‑1 (MQ=255)                                                                                                                                                                                   gCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGCACCACGCCCAGGATCGTGTAATCATCCGGCGTGCTCAGTTCAAATTCAGt                                               <  2:722743/238‑1 (MQ=255)                                                                                                                                                                                   gCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCAAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGCACCACGCCCAGGATCGTGTAATCATCCGGCGTGCTCAGTTCAAATTCAGt                                               <  2:604582/238‑1 (MQ=255)                                                                                                                                                                                     aCTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGCACCACGCCCGGGATCGTGTAATCATCCGGCGTGCTCAGTTCAAATTCAGTGcc                                            >  1:212269/1‑239 (MQ=255)                                                                                                                                                                                        gCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGCACCACGCCCAGGATCGTGTAATCATCCGGCGTGCTCAGTTCAAATTCAg                                                <  2:187570/232‑1 (MQ=255)                                                                                                                                                                                                 tGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGGTATg                                                                                                                                         >  2:419450/1‑134 (MQ=255)                                                                                                                                                                                                             ggATTCTCGCAAAACGTCGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGCACCACGCCCAGGATCGTGTAATCATCCGGCGTGCTCAGTTCAAATTCAGTGCCACCAGGGCGGGTGGCGATTTCcgc                    <  2:69565/239‑1 (MQ=255)                                                                                                                                                                                                                              cGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGCACCACGCCCAGGATCGTGTAATCATCCGGCGTGCTCAGTTCAAATTCAGTGCCACCAGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCg   >  2:222966/1‑239 (MQ=255)                                                                                                                                                                                                                              cGCCAGCACCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGCACCACGCCCAGGATCGTGTAATCATCCGGCGTGCTCAGTTCAAATTCAGTGCCACCAGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGa  <  2:521505/240‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         TGATTACACAAATCCAGCACCGTCACCGGTTCGGACATGTTTTCCAGCACATATTCACGGGCGCGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACCATTGGTTGCGCTTCTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACAAAGCCCCACAGCGCGGCTTTATGCTGCTCTTTCACTTCCAGCGCCGACTGGTTACGCAACATATGTAATACCCGATCCGGGTTATGCAAAAAGTTAGCCTGCCGGGTGATGACATCTTCAGAAAGCACCACGCCCAGGATCGTGTAATCATCCGGCGTGCTCAGTTCAAATTCAGTGCCACCAGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGA  >  NC_000913/2554389‑2554849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑