Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,570,488	A→G	C422R (TGC→CGC)	eutE ←	putative aldehyde dehydrogenase, ethanolamine utilization protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,570,488	0	A	G	100.0%	12.7 / NA	5	C422R (TGC→CGC)	eutE	putative aldehyde dehydrogenase, ethanolamine utilization protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/1);  total (4/1)

CGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATGGCATCCGCCACGTTGGCGACGCGCACGACGGGCAACACCGGCATCATCAGTTCAGTCACGGCAAACGGATGTTCTGCGGTGGTTTCCACAAACAGCAGGCGCGTT  >  NC_000913/2570385‑2570720                                                                                                        |                                                                                                                                                                                                                                         cGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCGCGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATGGCATCCGcc                                                                                                  >  2:473353/1‑240 (MQ=255)                                                                                                 gCAATGCGCGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATGGCATCCGCCACGTTGGCGACGCGCACGACGGGCAACACCGGcatcat                                                            <  2:582683/182‑1 (MQ=255)                                                                                                 gCAATGCGCGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATGGCATCCGCCACGTTGGCGACGCGCACGACGGGCAACACCGGCATCATCAGTTCAGTCACGGCAAACGGATGTTCTGCGGTGGTTTCCACAAACAGCAGGCGCGtt  >  1:253571/1‑240 (MQ=255)                                                                                                 gCAATGCGCGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATGGCATCCGCCACGTTGGCGACGCGCACGACGGGCAACACCGGCATCATCAGTTCAGTCACGGCAAACGGATGTTCTGCGGTGGTTTCCACAAACAGCAGGCGCGtt  >  1:373163/1‑240 (MQ=255)                                                                                                 gCAATGCGCGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATGGCATCCGCCACGTTGGCGACGCGCACGACGGGCAACACCGGCATCATCAGTTCAGTCACGGCAAACGGATGTTCTGCGGTGGTTTCCACAAACAGCAGGCGCGtt  >  2:162434/1‑240 (MQ=255)                                                                                                        |                                                                                                                                                                                                                                         CGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCATCTGGTTCATGTTTTCGATGTTGCGCGAGTGCATTGCCGCCGTGTGGTGGCAACCGCCTTCCAGTTTCACCGCTAGCGCAATGGCATCCGCCACGTTGGCGACGCGCACGACGGGCAACACCGGCATCATCAGTTCAGTCACGGCAAACGGATGTTCTGCGGTGGTTTCCACAAACAGCAGGCGCGTT  >  NC_000913/2570385‑2570720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑