Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,836,975	T→C	T151A (ACC→GCC)	hypF ←	carbamoyltransferase HypF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,836,975	0	T	C	100.0%	203.2 / NA	55	T151A (ACC→GCC)	hypF	carbamoyltransferase HypF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/28);  total (27/28)

CAAGATGAAATCCGCCAATCCCTTTGATGGCGACAATTTTGCCCATTTTTAACTGTGCGATAGCTGCCTGTAATGCCGCCTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGTAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTC  >  NC_000913/2836747‑2837197                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                cAAGATGAAATCCGCCAATCCCTTTGATGGCGACAATTTTGCCCATTTTTAACTGTGCGATAGCTGCCTGTAATGCCGCCTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCgg                                                                                                                                                                                                                       <  1:415039/239‑1 (MQ=255)            ccGCCAATCCCTTTGATGGCGACAATTTTGCCCATTTTTAACTGTGCGATAGCTGCCTGTAATGCCGCCTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATg                                                                                                                                                                                                           <  1:335683/240‑1 (MQ=255)               ccAATCCCTTTGATGGCGACAATTTTGCCCATTTTTAACTGTGCGATAGCTGCCTGTAATGCCGCCTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGgc                                                                                                                                                                                                         >  1:304767/1‑239 (MQ=255)                               cGACAATTTTGCCCATTTTTAACTGTGCGATAGCTGCCTGTAATGCCGCCTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGaaa                                                                                                                                                                                         >  2:255009/1‑239 (MQ=255)                                          cccATTTTTAACTGTGCGATAGCTGCCTGTAATGCCGCCTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCa                                                                                                                                                                              <  1:648562/239‑1 (MQ=255)                                           ccATTTTTAACTGTGCGATAGCTGCCTGTAATGCCGCCTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGcc                                                                                                                                                                                 <  2:272711/235‑1 (MQ=255)                                              tttttACCTGTGCGATAGCGGCCTGTAATGCCGCCTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCa                                                                                                                                                                              <  2:121333/235‑1 (MQ=255)                                               ttttAACTGTGCGATAGCTGCCTGTAATGCCGCCTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTggg                                                                                                                                                                         >  1:673160/1‑239 (MQ=255)                                                        tgCGATAGCTGCCTGTAATGCCGCCTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGcc                                                                                                                                                                                 <  1:88056/222‑1 (MQ=255)                                                         gCGATAGCTGCCTGTAATGCCGCCTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATa                                                                                                                                                              >  2:292731/1‑240 (MQ=255)                                                            aTAGCTGCCTGTAATGCCGCCTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATaaa                                                                                                                                                            <  1:719842/239‑1 (MQ=255)                                                            aTAGCTGCCTGTAATGCCGCCTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATaaa                                                                                                                                                            <  1:579390/239‑1 (MQ=255)                                                                  gCCTGTAATGCCGCCTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAg                                                                                                                                                                    >  2:440986/1‑225 (MQ=255)                                                                             cgccTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTa                                                                                                                                                                       <  2:379963/211‑1 (MQ=255)                                                                             cgccTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTa                                                                                                                                                                       >  1:379968/1‑211 (MQ=255)                                                                                     gTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCt                                                                                                                                   <  2:399288/239‑1 (MQ=255)                                                                                           cATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTAt                                                                                                                            >  1:708416/1‑240 (MQ=255)                                                                                               ttCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCAt                                                                                                                        >  1:709162/1‑240 (MQ=255)                                                                                                   ccATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGgat                                                                                                                                                       <  1:494705/205‑1 (MQ=255)                                                                                                   ccATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCg                                                                                                                    <  1:486726/240‑1 (MQ=255)                                                                                                            aCCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCggcaaggcaa                                                                                                           >  1:577264/1‑240 (MQ=255)                                                                                                             cccATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGgcaaggcaag                                                                                                          <  1:160654/240‑1 (MQ=255)                                                                                                                        tGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAg                                                                                                 >  1:32586/1‑238 (MQ=255)                                                                                                                          cGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCTTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGt                                                                                                >  2:529951/1‑237 (MQ=255)                                                                                                                            ggCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTAtt                                                                                                                           >  2:91569/1‑208 (MQ=255)                                                                                                                            ggCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCgg                                                                                           >  2:649984/1‑240 (MQ=255)                                                                                                                                      cGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGaa                                                                                  >  1:298489/1‑239 (MQ=255)                                                                                                                                       gggCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACa                                                                                >  1:45391/1‑240 (MQ=255)                                                                                                                                        ggCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACaa                                                                               >  2:603035/1‑240 (MQ=255)                                                                                                                                            ggCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTg                                                                          <  1:160607/240‑1 (MQ=255)                                                                                                                                                  cGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCg                                                                        >  2:76080/1‑236 (MQ=255)                                                                                                                                                       gggCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCt                                                                                                                                   <  2:667648/173‑1 (MQ=255)                                                                                                                                                       gggCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATgg                                                               >  1:36310/1‑240 (MQ=255)                                                                                                                                                        ggCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGACGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGt                                                              <  1:262314/240‑1 (MQ=255)                                                                                                                                                              gAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTgccgcc                                                        >  1:289362/1‑240 (MQ=255)                                                                                                                                                               aaGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTGCCGCCt                                                       <  1:442202/240‑1 (MQ=255)                                                                                                                                                                aGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCTTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTGCCGCCt                                                       <  1:529960/239‑1 (MQ=255)                                                                                                                                                                     cgaTCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTGCCGCCTGTGct                                                  <  2:16491/239‑1 (MQ=255)                                                                                                                                                                                    cGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTgccgc                                                         <  2:230415/217‑1 (MQ=255)                                                                                                                                                                                     ggTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACt                                 <  1:352262/240‑1 (MQ=255)                                                                                                                                                                                          tctTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGt                                                              >  2:465588/1‑206 (MQ=255)                                                                                                                                                                                           ctTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTgccgc                                                         <  2:593058/210‑1 (MQ=255)                                                                                                                                                                                            tttGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTggg                          <  2:304764/240‑1 (MQ=255)                                                                                                                                                                                               gTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAg                       >  1:123116/1‑240 (MQ=255)                                                                                                                                                                                               gTCACAGGCCGGAAATAGCGGAAACGCCGCAATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTggg                          <  2:733203/237‑1 (MQ=255)                                                                                                                                                                                                 cacaGGCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGt                      >  2:263298/1‑239 (MQ=255)                                                                                                                                                                                                      gCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCg                               >  2:481380/1‑225 (MQ=255)                                                                                                                                                                                                      gCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGAc                >  1:710706/1‑240 (MQ=255)                                                                                                                                                                                                      gCCGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGAc                >  1:588345/1‑240 (MQ=255)                                                                                                                                                                                                       ccGGACATAGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAATTTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGAc                <  1:721969/240‑1 (MQ=255)                                                                                                                                                                                                          gaaaTAGCGGAAACGCCGCAATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCg                               <  2:265239/218‑1 (MQ=255)                                                                                                                                                                                                               aGCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATaaa        <  2:83767/239‑1 (MQ=255)                                                                                                                                                                                                                gCGGAAACGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCAGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTa                                                                      >  1:625609/1‑176 (MQ=255)                                                                                                                                                                                                                     aaCGCCGCCATCACGGCAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGcgc  >  2:437144/1‑237 (MQ=255)                                                                                                                                                                                                                           gccATCACGGCAAACGGGCGGTAGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGt                                                              <  1:484759/173‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                CAAGATGAAATCCGCCAATCCCTTTGATGGCGACAATTTTGCCCATTTTTAACTGTGCGATAGCTGCCTGTAATGCCGCCTCTTGTTCCGCATGTTCACCATGACTTACCCATTCAAGATGCGGGCCACACTCCGGGCAGGCCACCGGCTGGGCGTGGAAGCGACGATCGAGCGGGTCACGGTACTCTTTGTCACAGGCCGGACATAGCGGAAACGCCGCCATCACGGTAAACGGGCGGTCGTAAGGCATGGCGCGAATAATGGTGAAACGCGGGCCGCAGTGGGTACAGTTGATAAACGGATAACGATAACGCCGTTCGCCTGGGGTATTCATTTCGGCAAGGCAAGCAGGGCAAGTAGCGGCATCGGGAACAA‑TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGTATAGTGAACTCGGTGGGCAGTTGTGACCAGATAAACGGCTC  >  NC_000913/2836747‑2837197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑