Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,258,818	G→T	G10C (GGT→TGT)	ychH →	stress‑induced protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,258,818	0	G	T	100.0%	337.1 / NA	84	G10C (GGT→TGT)	ychH	stress‑induced protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (43/41);  total (43/41)

AAATGCAGATAACGTAATAATTGCCTGAGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAACGTTGCCG  >  NC_000913/1258592‑1259041                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                aaaTGCAGATAACGTAATAATTGCCTGAGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCa                                                                                                                                                                                                                     <  2:248893/239‑1 (MQ=255) aaaTGCAGATAACGTAATAATTGCCTGAGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCa                                                                                                                                                                                                                     <  1:284764/239‑1 (MQ=255)           aaCGTAATAATTGCCTGAGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGt                                                                                                                                                                                                          <  1:515522/240‑1 (MQ=255)             cGTAATAATTGCCTGAGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAAc                                                                                                                                                                                                                            >  1:618995/1‑220 (MQ=255)                        ccTGAGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAAtg                                                                                                                                                                                              >  2:764844/1‑239 (MQ=255)                              tGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCg                                                                                                                                                                                       >  1:162140/1‑240 (MQ=255)                               ggACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCt                                                                                                                                                                                                        <  2:772237/222‑1 (MQ=255)                                  cTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGc                                                                                                                                                                                     >  2:697518/1‑238 (MQ=255)                                     ttAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTggg                                                                                                                                                                                >  1:678665/1‑240 (MQ=255)                                     ttAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTggg                                                                                                                                                                                <  2:746372/240‑1 (MQ=255)                                     ttAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTggg                                                                                                                                                                                >  1:253703/1‑240 (MQ=255)                                             tCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCaa                                                                                                                                                                        >  2:517163/1‑240 (MQ=255)                                             tCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCaa                                                                                                                                                                        >  1:246571/1‑240 (MQ=255)                                               aaGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTggg                                                                                                                                                                                <  2:64717/230‑1 (MQ=255)                                               aaGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAAt                                                                                                                                                                       >  2:214708/1‑239 (MQ=255)                                               aaGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATc                                                                                                                                                                      >  2:532494/1‑240 (MQ=255)                                                 ggTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTc                                                                                                                                                                          <  2:442359/234‑1 (MQ=255)                                                     ttCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAAc                                                                                                                                                                >  2:47459/1‑240 (MQ=255)                                                              ttATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACca                                                                                                                                                       <  1:698588/240‑1 (MQ=255)                                                              ttATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACca                                                                                                                                                       <  2:115669/240‑1 (MQ=255)                                                              ttATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACca                                                                                                                                                       <  2:249643/240‑1 (MQ=255)                                                               tATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACcac                                                                                                                                                      <  2:300907/240‑1 (MQ=255)                                                                    gTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGttt                                                                                                                                                 >  1:742403/1‑240 (MQ=255)                                                                              gttgAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACca                                                                                                                                                       <  1:563915/224‑1 (MQ=255)                                                                                    aTAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTac                                                                                                                                                                                                                             >  2:499725/1‑147 (MQ=255)                                                                                           ttGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGcaca                                                                                                                          <  1:428203/240‑1 (MQ=255)                                                                                           ttGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGcaca                                                                                                                          <  2:409490/240‑1 (MQ=255)                                                                                           ttGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGcaca                                                                                                                          <  1:96043/240‑1 (MQ=255)                                                                                           ttGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGcaca                                                                                                                          <  1:291793/240‑1 (MQ=255)                                                                                           ttGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGcaca                                                                                                                          <  2:327914/240‑1 (MQ=255)                                                                                                aTTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTATTATGCCCCAGTATTTCGCACATGGTg                                                                                                                     <  1:534400/240‑1 (MQ=255)                                                                                                aTTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTg                                                                                                                     <  1:281244/240‑1 (MQ=255)                                                                                                   gtgATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCa                                                                                                                   >  2:613523/1‑239 (MQ=255)                                                                                                     gATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTg                                                                                                                >  1:518431/1‑240 (MQ=255)                                                                                                      aTCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGt                                                                                                                                                                                                          <  1:473537/149‑1 (MQ=255)                                                                                                      aTCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGt                                                                                                                                                                                                          >  2:473530/1‑149 (MQ=255)                                                                                                        cACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGCCATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTc                                                                                                                                                                                                         <  2:349336/148‑1 (MQ=255)                                                                                                        cACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTg                                                                                                                     >  1:100764/1‑232 (MQ=255)                                                                                                           gCCCGCACCTAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCaa                                                                                                                                                                 <  1:331039/185‑1 (MQ=255)                                                                                                           gCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTaa                                                                                                            <  2:536643/238‑1 (MQ=255)                                                                                                             ccGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTg                                                                                                                     <  2:491903/227‑1 (MQ=255)                                                                                                                      aCCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGcaca                                                                                                                          <  1:108635/213‑1 (MQ=255)                                                                                                                            tGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGcaca                                                                                                                          <  2:675740/207‑1 (MQ=255)                                                                                                                             gggTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTCCCACAGTTTAATATGCCCCAGTAt                                                                                                                                  >  2:91393/1‑198 (MQ=255)                                                                                                                                 gttgtCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTctct                                                                                    >  1:426260/1‑240 (MQ=255)                                                                                                                                   tgtCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGc                                                                                                                             >  2:390147/1‑197 (MQ=255)                                                                                                                                   tgtCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGc                                                                                                                             <  1:390154/197‑1 (MQ=255)                                                                                                                                     tCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAg                                                                                                                                                    >  1:270734/1‑172 (MQ=255)                                                                                                                                        ataCTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTAtt                                                                                                                                                                           >  1:247093/1‑146 (MQ=255)                                                                                                                                        ataCTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTAtt                                                                                                                                                                           <  2:247090/146‑1 (MQ=255)                                                                                                                                          aCTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCgg                                                                           >  1:704475/1‑240 (MQ=255)                                                                                                                                              tACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTg                                                                        >  1:75880/1‑239 (MQ=255)                                                                                                                                              tACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTg                                                                        >  2:60423/1‑239 (MQ=255)                                                                                                                                              tACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTg                                                                        >  2:566444/1‑239 (MQ=255)                                                                                                                                              tACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTg                                                                        >  2:285933/1‑239 (MQ=255)                                                                                                                                              tACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTg                                                                        >  1:147623/1‑239 (MQ=255)                                                                                                                                              tACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTg                                                                        >  1:372730/1‑239 (MQ=255)                                                                                                                                              tACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGc                                                                       >  1:33378/1‑240 (MQ=255)                                                                                                                                              tACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGc                                                                       >  1:36314/1‑240 (MQ=255)                                                                                                                                               acacATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGcc                                                                      >  1:70565/1‑240 (MQ=255)                                                                                                                                               acacATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGc                                                                       <  2:246568/239‑1 (MQ=255)                                                                                                                                                       ggAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTgg                                                              <  1:641963/240‑1 (MQ=255)                                                                                                                                                       ggAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTgg                                                              <  1:632828/240‑1 (MQ=255)                                                                                                                                                          agagGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAg                                                                                                                                                    >  1:177662/1‑151 (MQ=255)                                                                                                                                                          agagGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCgg                                                           >  1:600860/1‑240 (MQ=255)                                                                                                                                                            agGGGTATTCCCTGTTACAACCCAGAAAGTCCCGGAGGGGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTGACGGCGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTAcc                                                                                                                                                        >  2:311840/1‑145 (MQ=255)                                                                                                                                                               ggTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTg                                                                                                                     <  2:91644/177‑1 (MQ=255)                                                                                                                                                                 tATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGaa                                                    >  2:187847/1‑240 (MQ=255)                                                                                                                                                                  aTTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAAc                                                   <  2:58008/240‑1 (MQ=255)                                                                                                                                                                       cTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTgg                                                              <  2:518424/224‑1 (MQ=255)                                                                                                                                                                        tGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGc                                                                                                                                                                                                                        >  2:411209/1‑69 (MQ=255)                                                                                                                                                                                  cAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGtt                                    >  1:19642/1‑239 (MQ=255)                                                                                                                                                                                       aGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACtggt                              <  1:47459/240‑1 (MQ=255)                                                                                                                                                                                        gTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGCGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACtggt                              <  1:325000/239‑1 (MQ=255)                                                                                                                                                                                          tCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTggg                           >  1:279377/1‑240 (MQ=255)                                                                                                                                                                                           ccGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGt                          >  1:367722/1‑240 (MQ=255)                                                                                                                                                                                            cGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGtt                         <  1:697527/240‑1 (MQ=255)                                                                                                                                                                                                   gACATATGAAACGCAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGTTGCCCg                                                                    >  2:295679/1‑190 (MQ=255)                                                                                                                                                                                                                cAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACtggtgg                            >  2:275692/1‑217 (MQ=255)                                                                                                                                                                                                                cAAAAACGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAACGTTg     <  1:517170/240‑1 (MQ=255)                                                                                                                                                                                                                   aaaCGCTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAACGTTgccg  <  1:699868/240‑1 (MQ=255)                                                                                                                                                                                                                        cTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCtggtgg                                                                                                                                                                                                   <  2:433016/42‑1 (MQ=255)                                                                                                                                                                                                                        cTTCGTTACTCTGTAACGTGCTCATGGGGTTGGGTCtggtgg                                                                                                                                                                                                   >  1:433023/1‑42 (MQ=255)                                                                                                                                                                                                                           cGTTACTCTGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGc                                                         <  2:666391/177‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                AAATGCAGATAACGTAATAATTGCCTGAGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTACCACAGTTTAATATGCCCCAGTATTTCGCACATGGTGCAGTGCTAAGTATTTTCGTCGGTGCCATTCTCTGGCTGGCGGGTGCCCGTGTTGGCGGGCATGAACAGGTGTGCGACCGTTACTGGTGGGTTCGCCACTATGACAAACGTTGCCG  >  NC_000913/1258592‑1259041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑