New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? BN000925 = 45459320 (1.190)7 (0.030) 7/414 18.3 2.2% coding (585/2214 nt) traE TraE putative helicase
?BN000925 = 45489 316 (1.220)coding (555/2214 nt) traE TraE putative helicase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGTGGTCACGGACACGATGCGGGCGGGTTGGCCGCTCACGGCGGCCGCGATGCCCTCGGCGTGCTCCTTGTTGCTGAGGCGGCCTTTCTCGTCCACCGGATCGCCGTCGGCGACCTGGTAACGGGCCGGGAACTGAATGCCCTCGACCTCGAACTGGCCGTTCACCAGGTAGTAGTAGGTTTTCTGGTGGGCCGCGTTCTCGCGGCAACGGCGCACGACAAGGCCCAGGATCGGAGTCTGCACGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  BN000925/45213‑45459
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcacgcgcGAAGGGCTATCAGGGGCTGTTGAGCGTGGGGCGCGTGCAGACTCCGATCCTGGGCCTTGTCGTGCGCCGTTGCCGCGAGAACGCGGCCCACCAGAAAACCTACTACTACCTGGTGAACGGCCAGTTCGAGGTCGAGGGCATTCAGTTCCCGGCCCGTTACCAGGTCGCCGACGGCGATCCGGTGGACGAGAAAGGCCGCCTCAGCAACAAGGAGCACGCCGAGGGCATCGCGG  <  BN000925/45489‑45257
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
GGTGGTCACGGACACGATGCGGGCGGGTTGGCCGCTCACGGCGGCCGCGATGCCCTCGGCGTGCTCCTTGTTGCTGAGGCGGCCTTTCTCGTCCACCGGATCGCCGTCGGCGACCTGGTAACGGGCCGGGAACTGAATGCCCTCGACCTCGAACTGGCCGTTCACCAGGTAGTAGTAGGTTTTCTGGTGGGCCGCGTTCTCGCGGCAACGGCGCACGACAAGGCCCAGGATCGGAGTCTG                                                                                                                                                                                                                                                  >  2:392359/1‑240
 GTGGTCACGGACACGATGCGGGCGGGTTGGCCGCTCACGGCGGCCGCGATGCCCTCGGCGTGCTCCTTGTTGCTGAGGCGGCCTTTCTCGTCCACCGGATCGCCGTCGGCGACCTGGTAACGGGCCGGGAACTGAATGCCCTCGACCTCGAACTGGCCGTTCACCAGGTAGTAGTAGGTTTTCTGGTGGGCCGCGTTCTCGCGGCAACGGCGCACGACAAGGCCCAGGATCGGAGTCTGC                                                                                                                                                                                                                                                 <  1:465121/240‑1
     TCACGGACACGATGCGGGCGGGTTGGCCGCTCACGGCGGCCGCGATGCCCTCGGCGTGCTCCTTGTTGCTGAGGCGGCCTTTCTCGTCCACCGGATCGCCGTCGGCGACCTGGTAACGGGCCGGGAACTGAATGCCCTCGACCTCGAACTGGCCGTTCACCAGGTAGTAGTAGGTTTTCTGGTGGGCCGCGTTCTCGCGGCAACGGCGCACGACAAGGCCCAGGATCGGAGTCTGCACG                                                                                                                                                                                                                                              <  2:188117/239‑1
        CGGACACGATGCGGGCGGGTTGGCCGCTCACGGCGGCCGCGATGCCCTCGGCGTGCTCCTTGTTGCTGAGGCGGCCTTTCTCGTCCACCGGATCGCCGTCGGCGACCTGGTAACGGGCCGGGAACTGAATGCCCTCGACCTCGAACTGGCCGTTCACCAGGTAGTAGTAGGTTTTCTGGTGGGCCGCGTTCTCGCGGCAACGGCGCACGACAAGGCCCAGGATCGGAGTCTGCACGC                                                                                                                                                                                                                                             <  2:586902/237‑1
                    GGGCGGGTTGGCCGCTCACGGCGGCCGCGATGCCCTCGGCGTGCTCCTTGTTGCTGAGGCGGCCTTTCTCGTCCACCGGATCGCCGTCGGCGACCTGGTAACGGGCCGGGAACTGAATGCCCTCGACCTCGAACTGGCCGTTCACCAGGTAGTAGTAGGTTTTCTGGTGGGCCGCGTTCTCGCGGCAACGGCGCACGACAAGGCCCAGGATCGGAGTCTGCACGCGCGAAGGGCTATCAG                                                                                                                                                                                                                              <  2:481114/240‑1
                                                                        GCTGAGGCGGCCTTTCTCGTCCACCGGATCGCCGTCGGCGACCTGGTAACGGGCCGGGAACTGAATGCCCTCGACCTCGAACTGGCCGTTCACCAGGTAGTAGTAGGTTTTCTGGTGGGCCGCGTTCTCGCGGCAACGGCGCACGACAAGGCCCAGGATCGGAGTCTGCACGCGCGAAGGGCTATCAGGGGCTGTTGAGCGTGGGGCGCGTGCAGACTCCGATCCTGGGCCTTGTCGTG                                                                                                                                                                           <  1:494423/239‑1
                                                                                                    TCGCCGTNGGCGACCTGGTAACGGGCCGGGAACTGAATGCCCTCGACCTCGAACTGGCCGTTCACCAGGTAGTAGTAGGTTTTCTGGTGGGCCGCGTTCTCGCGGCAACGGCGCACGACAAGGCCCAGGATCGGAGTCTGCACGCGCGAAGGGCTATCAGG                                                                                                                                                                                                                             <  2:228960/161‑1
                                                                                                     CGCCGTCGGCGACCTGGTAACGGGCCGGGAACTGAATGCCCTCGACCTCGAACTGGCCGTTCACCAGGTAGTAGTAGGTTTTCTGGTGGGCCGCGTTCTCGCGGCAACGGCGCACGACAAGGCCCAGGATCGGAGTCTGCA                                                                                                                                                                                                                                                >  2:144752/1‑141
                                                                                                                                                                                      TCTGGTGGGCCGCGTTCTCGCGGCAACGGCGCACGACAAGGCCCAGGATCGGAGTCTGCACGCGCGAAGGGCTATCAGGGGCTGTTGAGCGTGGGGCGCGTGC                                                                                                                                                                                                     <  1:595988/103‑1
                                                                                                                                                                                      TCTGGTGGGCCGCGTTCTCGCGGCAACGGCGCACGACAAGGCCCAGGATCGGAGTCTGCACGCGCGAAGGGCTATCAGGGGCTGTTGAGCGTGGGGCGCGTGC                                                                                                                                                                                                     >  2:595979/1‑103
                                                                                                                                                                                                                                    GATCGGAGTCTGCACGCGCGAAGGGCTATCAGGGGCTGTTGAGCGTGGGGCGCGTGCAGACTCCGATCCTGGGCCTTGTCGTGCGCCGTTGCCGCGAGAACGCGGCCCACCAGAAAACCTACTACTACCTGGTGAACGGCCAGTTCGAGGTCGAGGGCATTCAGTTCCCGGCCCGTTACCAGGTCGCCGACGGCGATCCGGTGGACGAGAAAGGCCGCCTCAGCAACAAGGAGCACGCC               >  1:189848/1‑239
                                                                                                                                                                                                                                        GGAGTCTGCACGCGCGAAGGGCTATCAGGGGCTGTTGAGCGTGGGGCGCGTGCAGACTCCGATCCTGGGCCTTGTCGTGCGCCGTTGCCGCGAGAACGCGGCCCACCAGAAAACCTACTACTACCTGGTGAACGGCCAGTTCGAGGTCGAGGGCATTCAGTTCCCGGCCCGTTACCAGGTCGCCGACGGCGATCCGGTGGACGAGAAAGGCCGCCTCAGCAACAAGGAGCACGCCGAGGG          >  2:509694/1‑240
                                                                                                                                                                                                                                               GCACGCGCGAAGGGCTATCAGGGGCTGTTGAGCGTGGGGCGCGTGCAGACTCCGATCCTGGGCCTTGTCGTGCGCCGTTGCCGCGAGAACGCGGCCCACCAGAAAACCTACTACTACCTGGTGAACGGCCAGTTCGAGGTCGAGGGCATTCAGTTCCCGGCCCGTTACCAGGTCGCCGACGGCGATCCGGTGGACGAGAAAGGCCGCCTCAGCAACAAGGAGCACGCCGAGGGCATCGCG   <  1:503106/240‑1
                                                                                                                                                                                                                                                  CGCGCGAAGGGCTATCAGGGGCTGTTGAGCGTGGGGCGCGTGCAGACTCCGATCCTGGGCCTTGTCGTGCGCCGTTGCCGCGAGAACGCGGCCCACCAGAAAACCTACTACTACCTGGTGAACGGCCAGTTCGAGGTCGAGGGCATTCAGTTCCCGGCCCGTTACCAGGTCGCCGACGGCGATCCGGTGGTCGAGAAAGGCCGCCT                                  <  2:394830/206‑1
                                                                                                                                                                                                                                                  CGCGCGAAGGGCTATCAGGGGCTGTTGAGCGTGGGGCGCGTGCAGACTCCGATCCTGGGCCTTGTCGTGCGCCGTTGCCGCGAGAACGCGGCCCACCAGAAAACCTACTACTACCTGGTGAACGGCCAGTTCGAGGTCGAGGGCATTCAGTTCCCGGCCCGTTACCAGGTCGCCGACGGCGATCCGGTGGACGAGAAAGGCCGCCTCAGCAACAAGGAGCACGCCGAGGGCATCGCGG  <  2:563471/238‑1
                                                                                                                                                                                                                                                     GCGAAGGGCTATCAGGGGCTGTTGAGCGTGGGGCGCGTGCAGACTCCGATCCTGGGCC                                                                                                                                                                                   <  2:129614/58‑1
                                                                                                                                                                                                                                                      CGAAGGGCTATCAGGGGCTGTTGAGCGTGGGGCGCGTGCAGACTCCGATCCTGGGCCTTGTCGTGCGCCGTTGCCGCGAGAACGCGGCCCACCAGAAAACCTACTACTACCTGGTGAACGGCCAGTTCGAGGTCGAGGGCATTCAGTTCCCGGCCCGTTACCAGGTCGCCGACGGCGATCCGGTGGACGAGAAAGGCCGCCTCAGCAACAAGGAGC                    <  2:586761/216‑1
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
GGTGGTCACGGACACGATGCGGGCGGGTTGGCCGCTCACGGCGGCCGCGATGCCCTCGGCGTGCTCCTTGTTGCTGAGGCGGCCTTTCTCGTCCACCGGATCGCCGTCGGCGACCTGGTAACGGGCCGGGAACTGAATGCCCTCGACCTCGAACTGGCCGTTCACCAGGTAGTAGTAGGTTTTCTGGTGGGCCGCGTTCTCGCGGCAACGGCGCACGACAAGGCCCAGGATCGGAGTCTGCACGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  BN000925/45213‑45459
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcacgcgcGAAGGGCTATCAGGGGCTGTTGAGCGTGGGGCGCGTGCAGACTCCGATCCTGGGCCTTGTCGTGCGCCGTTGCCGCGAGAACGCGGCCCACCAGAAAACCTACTACTACCTGGTGAACGGCCAGTTCGAGGTCGAGGGCATTCAGTTCCCGGCCCGTTACCAGGTCGCCGACGGCGATCCGGTGGACGAGAAAGGCCGCCTCAGCAACAAGGAGCACGCCGAGGGCATCGCGG  <  BN000925/45489‑45257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.