Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,023,510	T→C	R206R (CGT→CGC)	fliR →	flagellar biosynthesis protein FliR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,023,510	0	T	C	100.0%	190.1 / NA	51	R206R (CGT→CGC)	fliR	flagellar biosynthesis protein FliR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/22);  total (29/22)

TGGATATGCTGGCGTTACTGCTGTTCCTGACATTTAACGGTCATTTATGGTTGATTTCACTGCTGGTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAG  >  NC_000913/2023278‑2023744                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           tGGATATGCTGGCGTTACTGCTGTTCCTGACATTTAACGGTCATTTATGGTTGATTTCACTGCTGGTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGccc                                                                                                                                                                                                                                     <  1:481628/240‑1 (MQ=255)     tatGCTGGCGTTACTGCTGTTCCTGACATTTAACGGTCATTTATGGTTGATTTCACTGCTGGTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGc                                                                                                                                                                                                                                  >  2:506573/1‑239 (MQ=255)            gCGTTACTGCTGTTCCTGACATTTAACGGTCATTTATGGTTGATTTCACTGCTGGTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATcc                                                                                                                                                                                                                          >  2:162000/1‑240 (MQ=255)                 aCTGCTGTTCCTGACATTTAACGGTCATTTATGGTTGATTTCACTGCTGGTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCAtttt                                                                                                                                                                                                                     >  1:376000/1‑240 (MQ=255)                       gTTCCTGACATTTAACGGTCATTTATGGTTGATTTCACTGCTGGTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCAt                                                                                                                                                                                                                                          >  2:195800/1‑213 (MQ=255)                        ttCCTGACATTTAACGGTCATTTATGGTTGATTTCACTGCTGGTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGTAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTAtt                                                                                                                                                                                                              >  1:89484/1‑240 (MQ=255)                                 tttAACGGTCATTTATGGTTGATTTCACTGCTGGTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCa                                                                                                                                                                                                     <  1:223910/240‑1 (MQ=255)                                                   ttGATTTCACTGCTGGTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGc                                                                                                                                                                                   <  1:344534/240‑1 (MQ=255)                                                     gATTTCACTGCTGGTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCAt                                                                                                                                                                                 <  1:162844/240‑1 (MQ=255)                                                           aCTGCTGGTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCttt                                                                                                                                                                           >  2:143683/1‑240 (MQ=255)                                                                 ggTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATgg                                                                                                                                                                      <  1:98886/239‑1 (MQ=255)                                                                       tACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCAtt                                                                                                                                                               >  2:502278/1‑240 (MQ=255)                                                                                 acCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGtt                                                                                                                                                      >  2:586549/1‑239 (MQ=255)                                                                                    cTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAAtt                                                                                                                                                  >  2:288163/1‑240 (MQ=255)                                                                                     tGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAAtt                                                                                                                                                  >  1:62019/1‑239 (MQ=255)                                                                                      gCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTg                                                                                                                                                 >  2:395682/1‑239 (MQ=255)                                                                                       ccGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGc                                                                                                                                                <  1:142055/239‑1 (MQ=255)                                                                                                 gCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACTCTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCg                                                                                                                                     <  1:355342/240‑1 (MQ=255)                                                                                                 gCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCg                                                                                                                                     <  1:282107/240‑1 (MQ=255)                                                                                                 gCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCg                                                                                                                                     <  1:19044/240‑1 (MQ=255)                                                                                                  cGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCg                                                                                                                                     <  1:517227/239‑1 (MQ=255)                                                                                                      ccGTTGAACAGCAATGCGTTTCTGGCACTCACCAGAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCg                                                                                                                                     <  2:197967/235‑1 (MQ=255)                                                                                                                                  tCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTgctgg                                                                                                    <  1:204765/240‑1 (MQ=255)                                                                                                                                   cACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACCTTTATTCAGTGAAATTTTTAATTTgctgg                                                                                                    >  2:346130/1‑239 (MQ=255)                                                                                                                                              ggAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCAtt                                                                                                                                                               >  2:28745/1‑169 (MQ=255)                                                                                                                                              ggAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATattatt                                                                                         >  1:36256/1‑239 (MQ=255)                                                                                                                                               gAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGatat                                                                                             <  2:589854/234‑1 (MQ=255)                                                                                                                                                  tttGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTg                                                                                     >  1:129386/1‑239 (MQ=255)                                                                                                                                                          tCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCAt                                                                            >  2:156025/1‑240 (MQ=255)                                                                                                                                                                 cGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCAt                                                                            <  1:331555/233‑1 (MQ=255)                                                                                                                                                                     cTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAAtt                                                                  >  1:496038/1‑239 (MQ=255)                                                                                                                                                                         tGCTGTCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATAttatta                                                                                        <  2:585174/213‑1 (MQ=255)                                                                                                                                                                         tGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGa                                                                                                                                                                                                           >  1:444224/1‑98 (MQ=255)                                                                                                                                                                         tGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGa                                                                                                                                                                                                           <  2:444216/98‑1 (MQ=255)                                                                                                                                                                                   aCCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCa                                                   >  2:460110/1‑240 (MQ=255)                                                                                                                                                                                   aCCGCTCATTACTCTGCTGCTGACACTGAATCTAGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGACATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATc                                                    >  2:177531/1‑239 (MQ=255)                                                                                                                                                                                       cTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGt                                                <  1:610356/239‑1 (MQ=255)                                                                                                                                                                                            tACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTTGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCt                                          <  1:195804/240‑1 (MQ=255)                                                                                                                                                                                                 tgctgctgACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAgg                                      >  1:540766/1‑239 (MQ=255)                                                                                                                                                                                                   ctgctgACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTATCGTTTAt                                                     >  2:188905/1‑222 (MQ=255)                                                                                                                                                                                                   ctgctgACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTTTc                                            >  2:227091/1‑231 (MQ=255)                                                                                                                                                                                                      ctgACACTGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTAt                                 >  2:412131/1‑239 (MQ=255)                                                                                                                                                                                                          cacTGAATCTGCCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTg                                                                                                        <  1:283967/164‑1 (MQ=255)                                                                                                                                                                                                             tGAATCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGaaaaa                         >  2:100367/1‑240 (MQ=255)                                                                                                                                                                                                                 tCTGGCATTAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTAATGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAaataat                     >  2:422342/1‑240 (MQ=255)                                                                                                                                                                                                                         tAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATg                                                 <  2:505520/204‑1 (MQ=255)                                                                                                                                                                                                                         tAGGTTTACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACATACGa             >  2:602080/1‑240 (MQ=255)                                                                                                                                                                                                                             tttACTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACa          <  1:412139/239‑1 (MQ=255)                                                                                                                                                                                                                                aCTTAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCtt      >  2:482877/1‑240 (MQ=255)                                                                                                                                                                                                                                  ttAATCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTc     <  1:372886/239‑1 (MQ=255)                                                                                                                                                                                                                                     aTCGCATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAg  >  1:225530/1‑239 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           TGGATATGCTGGCGTTACTGCTGTTCCTGACATTTAACGGTCATTTATGGTTGATTTCACTGCTGGTCGATACCTTTCACACCCTGCCGATTGGTGGCGAACCGTTGAACAGCAATGCGTTTCTGGCACTCACCAAAGCAGGGAGTTTGATTTTCCTTAACGGGCTGATGCTGGCGTTACCGCTCATTACTCTGCTGCTGACACTGAATCTGGCATTAGGTTTACTTAATCGTATGGCCCCGCAATTATCCATTTTTGTTATTGGATTTCCATTAACTCTGACTGTCGGCATCTCTTTAATGGCGGCATTAATGCCGTTAATTGCACCTTTTTGCGAACATTTATTCAGTGAAATTTTTAATTTGCTGGCTGATATTATTAGTGAATTGCCATTAATATAATTCCGTAACGTTTATCATGTTATCCTAAGGATTATCCGAAAAATAATACCTACGAACATCTTCCAG  >  NC_000913/2023278‑2023744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑