Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,781,961	T→C	T256A (ACC→GCC)	ypjA ←	adhesin‑like autotransporter YpjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,781,961	0	T	C	100.0%	294.2 / NA	79	T256A (ACC→GCC)	ypjA	adhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/41);  total (38/41)

TTTGCCGAACCATATGCGTTTATGTCTAAATGACCACCGTTTTCCAGCAACACATTGTCTGCCACGTGATTGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACG  >  NC_000913/2781729‑2782187                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                   tttGCCGAACCATATGCGTTTATGTCTAAATGACCACCGTTTTCCAGCAACACATTGTCTGCCACGTGATTGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATc                                                                                                                                                                                                                              <  2:46579/239‑1 (MQ=255)            atatGCGTTTATGTCTAAATGACCACCGTTTTCCAGCAACACATTGTCTGCCACGTGATTGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGt                                                                                                                                                                                                                  <  1:8562/240‑1 (MQ=255)                 cGTTTATGTCTAAATGACCACCGTTTTCCAGCAACACATTGTCTGCCACGTGATTGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATATGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGccac                                                                                                                                                                                                             <  1:520648/240‑1 (MQ=255)                   tttATGTCTAAATGACCACCGTTTTCCAGCAACACATTGTCTGCCACGTGATTGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGccacca                                                                                                                                                                                                           <  1:466828/240‑1 (MQ=255)                                                  acacATTGTCTGCCACGTGATTGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGAc                                                                                                                                                                             <  2:548566/239‑1 (MQ=255)                                                          tCTGCCACGTGATTGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTg                                                                                                                                                                               >  2:126948/1‑229 (MQ=255)                                                              ccACGTGATTGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCaga                                                                                                                                                                 >  2:560921/1‑239 (MQ=255)                                                                aCGTGATTGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAgaga                                                                                                                                                               >  2:162419/1‑239 (MQ=255)                                                                    gATTGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAg                                                                                                                                                                                                     >  2:367799/1‑197 (MQ=255)                                                                          ggatggaGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCg                                                                                                                                                     <  2:598350/239‑1 (MQ=255)                                                                           gatggaGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGcc                                                                                                                                                   <  1:409563/240‑1 (MQ=255)                                                                                agaATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCa                                                                                                                                               >  2:576221/1‑239 (MQ=255)                                                                                agaATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCa                                                                                                                                               >  2:521650/1‑239 (MQ=255)                                                                                 gaATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCaa                                                                                                                                              <  1:182581/239‑1 (MQ=255)                                                                                  aaTGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAAtc                                                                                                                                            >  1:318296/1‑240 (MQ=255)                                                                                    tGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAAtcgt                                                                                                                                          >  2:247371/1‑240 (MQ=255)                                                                                                 ttCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCa                                                                                                                             <  1:189291/240‑1 (MQ=255)                                                                                                 ttCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCa                                                                                                                             <  1:320669/240‑1 (MQ=255)                                                                                                 ttCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCa                                                                                                                             <  1:553977/240‑1 (MQ=255)                                                                                                 ttCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCa                                                                                                                             <  1:450718/240‑1 (MQ=255)                                                                                                 ttCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCa                                                                                                                             <  1:173100/240‑1 (MQ=255)                                                                                                   cGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCAc                                                                                                                            <  2:42804/239‑1 (MQ=255)                                                                                                   cGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCAc                                                                                                                            >  2:9736/1‑239 (MQ=255)                                                                                                     tACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACgg                                                                                                                          <  1:556180/239‑1 (MQ=255)                                                                                                      aCCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTCTCAACAATTTGCGTGCCACCAGAATAGATATGCGTCTTTTTTGCCGTTGACTTAATATTCag                                                                                                                                                                  >  2:143045/1‑198 (MQ=255)                                                                                                      aCCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGt                                                                                                                                                         >  2:283841/1‑207 (MQ=255)                                                                                                      aCCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACgg                                                                                                                          >  1:83143/1‑238 (MQ=255)                                                                                                       ccGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGct                                                                                                                        <  2:509279/239‑1 (MQ=255)                                                                                                       ccGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGct                                                                                                                        <  2:308692/239‑1 (MQ=255)                                                                                                         gCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGctc                                                                                                                       >  2:452485/1‑238 (MQ=255)                                                                                                         gCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCtctc                                                                                                                     >  1:614171/1‑240 (MQ=255)                                                                                                              ccGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCatat                                                                                                                 <  2:157459/239‑1 (MQ=255)                                                                                                              ccGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCatat                                                                                                                 <  1:64992/239‑1 (MQ=255)                                                                                                               cgtcgtACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATAc                                                                                                               >  1:526388/1‑240 (MQ=255)                                                                                                                  cgtACCGTTAGTGTTAGTTTTT‑AAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATActct                                                                                                            <  1:451904/239‑1 (MQ=255)                                                                                                                   gtACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTAACACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGCTGACTTACTATTCAGAGACAGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCa                                                                                                                             <  1:34585/222‑1 (MQ=255)                                                                                                                    tACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCttt                                                                                                          >  1:486422/1‑240 (MQ=255)                                                                                                                         ttagtgttagtTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTc                                                                                                      >  1:38479/1‑239 (MQ=255)                                                                                                                         ttagtgttagtTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTc                                                                                                      >  1:505449/1‑239 (MQ=255)                                                                                                                           agtgttagtTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCATAGACt                                                                                                                                                             >  1:339032/1‑182 (MQ=255)                                                                                                                               ttagtTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTc                                                                                                                                                       >  1:154287/1‑184 (MQ=255)                                                                                                                               ttagtTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGcc                                                                                                <  1:476081/239‑1 (MQ=255)                                                                                                                                 agtTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCAtt                                                                                             >  2:365564/1‑240 (MQ=255)                                                                                                                                   tttttAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCatat                                                                                                                 <  1:509800/218‑1 (MQ=255)                                                                                                                                         aaaTTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTg                                                                                                                                                                               <  2:558977/150‑1 (MQ=255)                                                                                                                                             tGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGgttgt                                                                                 <  1:517534/240‑1 (MQ=255)                                                                                                                                                       tGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACt                                                                       >  1:356009/1‑240 (MQ=255)                                                                                                                                                         cTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACCCTTTGCTCACCGCCATTTTTAATGgttgt                                                                                 <  1:429660/228‑1 (MQ=255)                                                                                                                                                              tAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGctct                                                                >  2:345372/1‑240 (MQ=255)                                                                                                                                                              tAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGctct                                                                >  1:571212/1‑240 (MQ=255)                                                                                                                                                              tAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGctct                                                                <  1:448618/240‑1 (MQ=255)                                                                                                                                                              tAACATTTGTTGCCGTACCACCCCTAACATCAAGCAAGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACCGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGctct                                                                >  1:262908/1‑240 (MQ=255)                                                                                                                                                                      gttgCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAAc                                                         >  1:84574/1‑239 (MQ=255)                                                                                                                                                                      gttgCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAAc                                                         >  2:560141/1‑239 (MQ=255)                                                                                                                                                                       ttgCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAATATCCGAGGTGCTGGCGTTATCAATAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAAtcgtc                                                                                                                                         <  2:483235/158‑1 (MQ=255)                                                                                                                                                                            gTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGt                                                  >  1:461357/1‑240 (MQ=255)                                                                                                                                                                            gTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGt                                                  >  1:434534/1‑240 (MQ=255)                                                                                                                                                                                  ccccTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGgtt                                                                                   <  2:293893/201‑1 (MQ=255)                                                                                                                                                                                  ccccTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGgtt                                                                                   >  1:293900/1‑201 (MQ=255)                                                                                                                                                                                  ccccTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGacca                                            <  1:343159/240‑1 (MQ=255)                                                                                                                                                                                             aaGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGt                                 >  1:550290/1‑240 (MQ=255)                                                                                                                                                                                              aGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGac                                              >  2:436281/1‑226 (MQ=255)                                                                                                                                                                                              aGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGtt                                >  1:548932/1‑240 (MQ=255)                                                                                                                                                                                                  cGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTgtgt                            >  1:409766/1‑240 (MQ=255)                                                                                                                                                                                                     caccaGAATAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCAACCAGAATAGATATGCGTATTTTTTGCCGTTGAATTACAATTCAGAGTCTGAGTTCCGCCTTCAATCGTCGTGTCCAg                                                                                                                                 >  2:392511/1‑136 (MQ=255)                                                                                                                                                                                                             tAAACTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGa                 <  1:307882/240‑1 (MQ=255)                                                                                                                                                                                                              aaaCTGTAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGa                 <  2:226680/239‑1 (MQ=255)                                                                                                                                                                                                               accTTCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGa                 <  2:600222/236‑1 (MQ=255)                                                                                                                                                                                                                  ttCAATAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTcc            <  1:367807/240‑1 (MQ=255)                                                                                                                                                                                                                      aTAACATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCtt          <  2:182337/238‑1 (MQ=255)                                                                                                                                                                                                                         aCATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGtact     <  2:356001/240‑1 (MQ=255)                                                                                                                                                                                                                          cATCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGtact     <  2:333006/239‑1 (MQ=255)                                                                                                                                                                                                                            tCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCActt                                      >  2:533307/1‑204 (MQ=255)                                                                                                                                                                                                                            tCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGa                 <  1:4605/225‑1 (MQ=255)                                                                                                                                                                                                                            tCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGa                 >  2:4605/1‑225 (MQ=255)                                                                                                                                                                                                                            tCCGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACg  >  2:456770/1‑240 (MQ=255)                                                                                                                                                                                                                             ccGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACg  <  2:505441/239‑1 (MQ=255)                                                                                                                                                                                                                              cGAGGTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGa                 <  1:70868/223‑1 (MQ=255)                                                                                                                                                                                                                                  gTGCTGGCGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGacca                                            <  2:285912/192‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                   TTTGCCGAACCATATGCGTTTATGTCTAAATGACCACCGTTTTCCAGCAACACATTGTCTGCCACGTGATTGTGGATGGAGAATGCACCTTCACTATTCGTACCGCTCACCGTCGTACCGTTAGTGTTAGTTTTTAAAATTGCACCATCGTGCTGGGTAACATTTGTTGCCGTACCACCCCTAACATCAAGCACGCCACCAGAATAAACTTCAATAACATCCGAGGTGCTGGTGTTATCAACAATTTGCGTGCCACCAGAATAGATATGCGTATTTTTTGCCGTTGACTTACTATTCAGAGACTGAGTTCCGCCTTCAATCGTCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACG  >  NC_000913/2781729‑2782187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑