Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,266,510	T→C	T186A (ACC→GCC)	tdcA ←	DNA‑binding transcriptional activator TdcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,266,510	0	T	C	100.0%	198.5 / NA	54	T186A (ACC→GCC)	tdcA	DNA‑binding transcriptional activator TdcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/19);  total (35/19)

AATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAA  >  NC_000913/3266290‑3266735                                                                                                                                                                                                                             |                                                                                                                                                                                                                                  aaTTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGAc                                                                                                                                                                                                                >  2:468885/1‑240 (MQ=255)  aTTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACt                                                                                                                                                                                                               <  1:112499/240‑1 (MQ=255)       ttAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTg                                                                                                                                                                                                          <  1:371104/240‑1 (MQ=255)            aCCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTAc                                                                                                                                                                                                     <  1:212477/240‑1 (MQ=255)                 aaGATGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGCCTCCAGCGTGGTGgcgc                                                                                                                                                                                                                                 <  2:589412/207‑1 (MQ=255)                                   aaGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTc                                                                                                                                                                                         >  2:95233/1‑229 (MQ=255)                                     ggAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAg                                                                                                                                                                             >  1:344767/1‑239 (MQ=255)                                                 aaGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGAc                                                                                                                                                                                     >  2:130368/1‑219 (MQ=255)                                                 aaGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGt                                                                                                                                                                >  2:158320/1‑240 (MQ=255)                                                 aaGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGt                                                                                                                                                                >  2:927/1‑240 (MQ=255)                                                 aaGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGt                                                                                                                                                                >  1:388475/1‑240 (MQ=255)                                                 aaGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGt                                                                                                                                                                >  2:479705/1‑240 (MQ=255)                                                         aGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCg                                                                                                                                                                                        >  2:540249/1‑208 (MQ=255)                                                         aGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCg                                                                                                                                                                                        <  1:540257/208‑1 (MQ=255)                                                              tGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGc                                                                                                                                                    >  1:602903/1‑239 (MQ=255)                                                                agaACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCtt                                                                                                                                                  <  2:195327/239‑1 (MQ=255)                                                                    cAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCAttt                                                                                                                                             <  1:519268/240‑1 (MQ=255)                                                                         ttATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGc                                                                                                                                         <  1:34138/239‑1 (MQ=255)                                                                                          tGAGTCCGTTTTAACGATGTTTTCAATACTGATTCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGa                                                                                                                                                       <  2:561964/208‑1 (MQ=255)                                                                                             gTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGT‑AGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCaaa                                                                                                                    <  2:242519/239‑1 (MQ=255)                                                                                               cGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCTCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCaaa                                                                                                                    <  1:369543/238‑1 (MQ=255)                                                                                                                       tGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCg                                                                                          >  1:201161/1‑240 (MQ=255)                                                                                                                         aTGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCgg                                                                                         >  1:188499/1‑239 (MQ=255)                                                                                                                          tGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCa                                                                                                                      >  1:73081/1‑209 (MQ=255)                                                                                                                          tGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCa                                                                                                                      <  2:73080/209‑1 (MQ=255)                                                                                                                                                 aGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTca                                                                <  1:543808/240‑1 (MQ=255)                                                                                                                                                  gCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTcat                                                               <  1:464735/240‑1 (MQ=255)                                                                                                                                                      ttCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTcatacat                                                           >  1:275816/1‑240 (MQ=255)                                                                                                                                                      ttCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTcataca                                                            >  2:25027/1‑239 (MQ=255)                                                                                                                                                        cGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAg                                                         >  1:108870/1‑240 (MQ=255)                                                                                                                                                                taCCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATCGAAACCTgc                                                 >  2:436785/1‑240 (MQ=255)                                                                                                                                                                taCCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTgc                                                 >  1:130078/1‑240 (MQ=255)                                                                                                                                                                taCCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTgc                                                 >  2:117673/1‑240 (MQ=255)                                                                                                                                                                taCCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTgc                                                 >  2:477915/1‑240 (MQ=255)                                                                                                                                                                  ccccATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTgcgc                                               >  2:359549/1‑240 (MQ=255)                                                                                                                                                                  ccccATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTgcg                                                <  2:130074/239‑1 (MQ=255)                                                                                                                                                                     cATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCttt                                            >  1:207168/1‑240 (MQ=255)                                                                                                                                                                     cATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCttt                                            >  1:189630/1‑240 (MQ=255)                                                                                                                                                                       tatTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGTACTCGAATAGCGGCTCAGCATGTAAATCCTGAAGCTTCATTTCTGCACTTAATGTACCAATCGCAAAATCCAGTCGACCGTAGCGGATTGCCGGTAAGAACGAAGACAGTTGCGTTTCATACATAGAAACCTGCGCTTTc                                           >  2:178498/1‑239 (MQ=255)                                                                                                                                                                       tatTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCg                                          >  1:42040/1‑240 (MQ=255)                                                                                                                                                                       tatTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTCAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCg                                          >  2:205546/1‑240 (MQ=255)                                                                                                                                                                             tttGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAac                                     >  1:415430/1‑239 (MQ=255)                                                                                                                                                                                 tgGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACctc                                >  2:213647/1‑240 (MQ=255)                                                                                                                                                                                 tgGCAACACCCACTGTTAGTTCTTCAACGACTCCTGCGTGGTGGCGCCCGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGACTCAAGATGGAAATCCTGAAGCCTCGTTTCt                                                                                                                                           >  2:106688/1‑133 (MQ=255)                                                                                                                                                                                   gccacacCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGc                                                                                                                                         <  2:503849/130‑1 (MQ=255)                                                                                                                                                                                             cTGTTCGTTCTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGCGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTg                            <  2:41694/232‑1 (MQ=255)                                                                                                                                                                                                ttcgttcTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTGTTGat                 <  2:186787/240‑1 (MQ=255)                                                                                                                                                                                                  cgttcTTCAACGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACAcc                                  >  2:233296/1‑221 (MQ=255)                                                                                                                                                                                                          aaCGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGAc       >  2:242136/1‑240 (MQ=255)                                                                                                                                                                                                            cGACTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACAt     <  2:344760/240‑1 (MQ=255)                                                                                                                                                                                                               cgccAGCGTGGTGGCGCAGGTGCATGTTCGGGACTTTCTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGt                                                                                        <  2:186789/152‑1 (MQ=255)                                                                                                                                                                                                               cTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATaaa  >  2:463287/1‑240 (MQ=255)                                                                                                                                                                                                               cTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATaaa  >  1:334796/1‑240 (MQ=255)                                                                                                                                                                                                               cTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATaaa  >  1:312923/1‑240 (MQ=255)                                                                                                                                                                                                               cTCCAGCGTGGTGGCGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATACTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTcgc                                                                                                   >  2:3296/1‑143 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                  AATTGATTAGAACCAAAAGGTGACGTCATATCACAAGGAATTACAGTTAAGAAATCAGCATTGAGAACAAGATTATAAATTGTCACGACTGAGTCGGTTTTAACGATGTTTTCAATACTGATGCCATTTCTTTGTAACGTAGTAAGCAGTTCGCTGTAGTACCCCATATTAGTTTGTGGCAACACCCACTGTTCGTTCTTCAACGACTCCAGCGTGGTGGTGCCGGTGCATGTTCGGGACTTACTGGCTACCAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAA  >  NC_000913/3266290‑3266735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑