Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,538,913	C→G	A510G (GCG→GGG)	yhgF →	putative RNA‑binding protein YhgF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,538,913	0	C	G	100.0%	197.6 / NA	52	A510G (GCG→GGG)	yhgF	putative RNA‑binding protein YhgF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (32/20);  total (32/20)

CCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAGCCTATCCGGTGGTGGAACGCATTCTGGCAGCAACACAGCAGGCA  >  NC_000913/3538683‑3539142                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      cccGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGAc                                                                                                                                                                                                                              >  2:148371/1‑240 (MQ=255)         tcGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCatga                                                                                                                                                                                                                      >  1:170159/1‑240 (MQ=255)          cGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCatga                                                                                                                                                                                                                      >  2:508303/1‑239 (MQ=255)                        gTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCg                                                                                                                                                                                                       <  1:12677/240‑1 (MQ=255)                                      aTCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGa                                                                                                                                                                                          <  1:211514/239‑1 (MQ=255)                                           ccgccgTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGaa                                                                                                                                                                                    >  1:32311/1‑240 (MQ=255)                                              ccgTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACg                                                                                                                                                                                  <  2:88298/239‑1 (MQ=255)                                                gTTTGCAGGATCCGCTGGCGGAGCTGGTGGAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTc                                                                                                                                                                               <  2:406388/240‑1 (MQ=255)                                                gTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTc                                                                                                                                                                               <  1:49504/240‑1 (MQ=255)                                                         aTCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGa                                                                                                                                                                      >  1:95998/1‑240 (MQ=255)                                                                   ggAGCGGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCa                                                                                                                                                            <  2:166048/240‑1 (MQ=255)                                                                    gAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCa                                                                                                                                                            <  1:441085/239‑1 (MQ=255)                                                                                         aaaTCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTg                                                                                                                                      <  1:324972/240‑1 (MQ=255)                                                                                               aTCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTc                                                                                                                                                                               <  2:207358/193‑1 (MQ=255)                                                                                                 cGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGaa                                                                                                                              <  1:473640/240‑1 (MQ=255)                                                                                                            aGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAg                                                                                                                    <  1:336032/239‑1 (MQ=255)                                                                                                              tATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTc                                                                                                                                                                               <  1:286359/178‑1 (MQ=255)                                                                                                              tATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTaaa                                                                                                                                                   >  1:101675/1‑206 (MQ=255)                                                                                                                   gCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCAGGGGCCGAAAGCCTTc                                                                                                                       >  2:411136/1‑229 (MQ=255)                                                                                                                       gACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGc                                                                                                        <  2:269470/240‑1 (MQ=255)                                                                                                                        aCGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCAGGGGCCGAAAGCCTTCGAGCAGTacg                                                                                                             >  1:219739/1‑234 (MQ=255)                                                                                                                              gCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCg                                                                                                                                          >  2:263000/1‑199 (MQ=255)                                                                                                                                      cAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTaa                                                                                          >  1:256649/1‑239 (MQ=255)                                                                                                                                                 cAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCCTTAACCACGGTGATaa                                                                              >  1:280136/1‑240 (MQ=255)                                                                                                                                                           gCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGa                                                                     <  2:226433/239‑1 (MQ=255)                                                                                                                                                                agtagAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTc                                                               <  1:385737/240‑1 (MQ=255)                                                                                                                                                                       gaCTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGtt                                                        >  1:205812/1‑240 (MQ=255)                                                                                                                                                                       gaCTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGtt                                                        >  1:448128/1‑240 (MQ=255)                                                                                                                                                                       gaCTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGtt                                                        >  1:304810/1‑240 (MQ=255)                                                                                                                                                                       gaCTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGtt                                                        >  1:506109/1‑240 (MQ=255)                                                                                                                                                                         cTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAACGCCTTCGAGCAg                                                                                                                 >  2:470994/1‑181 (MQ=255)                                                                                                                                                                         cTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTc                                                       >  2:427400/1‑239 (MQ=255)                                                                                                                                                                         cTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCa                                                      >  1:87172/1‑240 (MQ=255)                                                                                                                                                                         cTGCGTAAACGCCGTTGGCGTCGATCCCAACACTGCTTCCGTTCTGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGCTCAGTTCCAGAACCGTTAGCAACTGTTAAAAGTGAGGCGTCTggg                                                                                                                                    >  2:31149/1‑162 (MQ=255)                                                                                                                                                                            cGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCTGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCAccc                                                   >  1:148411/1‑240 (MQ=255)                                                                                                                                                                            cGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTTAGCAACTGTTAAAAGTGAGCCGTc                                                                                                                                        >  2:267932/1‑155 (MQ=255)                                                                                                                                                                            cGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCAccc                                                   >  1:200982/1‑240 (MQ=255)                                                                                                                                                                            cGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCACAAAACATCGTTGCCTGGCGCGATGAGAACgct                                                                                                                                                                                >  2:299057/1‑113 (MQ=255)                                                                                                                                                                                  cGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAGc                                             >  1:382775/1‑240 (MQ=255)                                                                                                                                                                                                aTCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAGCCTATc                                        <  2:389386/231‑1 (MQ=255)                                                                                                                                                                                                  ctcAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAGCCTATCCggtg                                   <  2:61701/234‑1 (MQ=255)                                                                                                                                                                                                           gCTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAGCCTATCCGGTGGTGGAACGCATTCt                     >  2:236408/1‑239 (MQ=255)                                                                                                                                                                                                            cTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTc                                                                                                                                                                               >  2:305761/1‑84 (MQ=255)                                                                                                                                                                                                            cTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTc                                                                                                                                                                               <  1:305767/84‑1 (MQ=255)                                                                                                                                                                                                            cTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAGCCTATCCGGTGGTGGAACGCATTCTgg                   >  1:428513/1‑240 (MQ=255)                                                                                                                                                                                                            cTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAGCCTATCCGGTGGTGGAACGCATTCTgg                   >  1:414435/1‑240 (MQ=255)                                                                                                                                                                                                            cTTCTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAGCCTATCCGGTGGTGGAACGCATTCTgg                   >  2:433542/1‑240 (MQ=255)                                                                                                                                                                                                               cTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTc                                                       <  2:251484/201‑1 (MQ=255)                                                                                                                                                                                                               cTGTTCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTc                                                       >  1:251489/1‑201 (MQ=255)                                                                                                                                                                                                                  ttCCGCTGTTAACCCGCGTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGAGGCGTCTACCGTTCACCCGGaa                                               >  1:318218/1‑206 (MQ=255)                                                                                                                                                                                                                                  cgTGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAGCCTATCCGGTGGTGGAACGCATTCTGGCAGCAACACAGCAGGCa  >  2:461478/1‑235 (MQ=255)                                                                                                                                                                                                                                    tGGGGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAGCCTATCCGGTGGTGGaa                             <  2:511423/206‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      CCCGGATCTCGACGTTTCGCTGCGTGGCGCGGTGTCTATCGCCCGCCGTTTGCAGGATCCGCTGGCGGAGCTGGTGAAAATCGATCCGAAATCTATCGGCGTAGGTCAGTATCAGCATGACGTCAGCCAGACGCAACTGGCCCGCAAACTGGACGCAGTAGTAGAAGACTGCGTAAACGCCGTTGGCGTCGATCTCAACACTGCTTCTGTTCCGCTGTTAACCCGCGTGGCGGGCCTGACGCGCATGATGGCGCAAAACATCGTTGCCTGGCGCGATGAGAACGGTCAGTTCCAGAACCGTCAGCAACTGTTAAAAGTGAGCCGTCTGGGGCCGAAAGCCTTCGAGCAGTGCGCGGGCTTCTTGCGCATTAACCACGGTGATAACCCGCTGGACGCGTCTACCGTTCACCCGGAAGCCTATCCGGTGGTGGAACGCATTCTGGCAGCAACACAGCAGGCA  >  NC_000913/3538683‑3539142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑