| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 999,590 | A→C | 5.6% | N63T (AAC→ACC) | ycbF → | putative periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 999,590 | 0 | A | C | 5.6% | 2218.0 / 14.0 | 703 | N63T (AAC→ACC) | ycbF | putative periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base A (480/174); new base C (3/46); total (483/220) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.31e-21 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 729 total aligned reads displayed.
GAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCA > CP009273/999526‑999657 | gaATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAAcc > 1:42739114/1‑67 (MQ=255) aTCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGC < 1:19358774‑M1/67‑1 (MQ=255) gATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCA > 1:23248337‑M1/1‑67 (MQ=255) gATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCA > 1:15013381‑M1/1‑67 (MQ=255) tatCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCgcccaac < 1:10592222‑M2/67‑8 (MQ=255) atCTATTTTACTGTCTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCTCCCACCGC < 1:15157754‑M1/68‑1 (MQ=255) atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCgcccaacgc > 1:6855023‑M2/1‑59 (MQ=255) atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGC < 1:43262294‑M1/68‑1 (MQ=255) tCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCgcccaacgcg > 1:29971854‑M2/1‑58 (MQ=255) tCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCgcccaacgcg < 1:36166903‑M2/68‑11 (MQ=255) cTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGT > 1:17089667‑M1/1‑68 (MQ=255) tATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGC < 1:21614871‑M1/65‑1 (MQ=255) aTTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCgccccacgcgttg < 1:24283572‑M2/68‑14 (MQ=255) ttttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCgcccaacgcgttgg < 1:39312885‑M2/68‑15 (MQ=255) ttttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGG > 1:32741496‑M1/1‑68 (MQ=255) ttttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGG > 1:7520929‑M1/1‑68 (MQ=255) tttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCgcccaacgcgttggg > 1:13433927‑M2/1‑53 (MQ=255) tttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGG > 1:7284657‑M1/1‑67 (MQ=255) tACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGcc > 1:27660718/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCACCGCGTTGGGCCg < 1:37630335/68‑1 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:27061325/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:28452792/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:32040005/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:6007150/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCAAACGCGTTGGGCCg > 1:1553/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg > 1:3508555/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg > 1:38961375/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg < 1:39875560/68‑1 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg < 1:28707666/68‑1 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg < 1:37233095/68‑1 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg > 1:42466032/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg > 1:9300707/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg < 1:19687571/68‑1 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg > 1:35379579/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg < 1:8291511/68‑1 (MQ=255) tGACTAAAACCAGTCAGGAATCCTGGCTTATTAACAGTAAACTCAACCGCCCAACGCGTTGGGCCggg < 1:12436761/68‑1 (MQ=255) cTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCgggggg < 1:22263056/68‑1 (MQ=255) cTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCgggggg > 1:37925042/1‑68 (MQ=255) tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCACCCGCCCACCGCGTTGGGCCGGGGGGGAAg < 1:11907125/68‑1 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCCACGCGTTGGGCCGGGGGGGAAGc > 1:10105968/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAGCGCGTTGGGCCGGGGGGGAAGc > 1:17850204/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:34675063/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:33393551/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:4141516/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:28122344/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:38659034/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:40721668/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:2461935/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:41974959/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:4368218/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:811170/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:8860808/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:16097497/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:13799987/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:14579680/1‑68 (MQ=255) aCCACTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGACCAACGCGTTGGGCCGGGGGGGAAGc > 1:18393591/1‑68 (MQ=255) ccAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCg > 1:32412455/1‑68 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCg > 1:40455953/1‑67 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCg > 1:3827780/1‑67 (MQ=255) cAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGAc < 1:1154980/68‑1 (MQ=255) cAGGAATCCTGGCTTATTAACAGAAAAAACACCCGCCCAACGCGTGGGGCCGGGGGGGAAGCGTCGa < 1:25153858/67‑1 (MQ=255) caTCCTGCCTTATTAACAGTAAATTCAACCCCCCAACCCGGGGGGCCGGGGGGGAAGCGTCGACAGt < 1:11085723/66‑1 (MQ=255) aaTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGt < 1:21230912/67‑1 (MQ=255) aTCCTGGCTTATTAACAGTAAACTCACCCGCCCAACGCGTTGGGCggttgattttactgttaataag < 1:20768803‑M1/67‑23 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:7685279/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:5679963/1‑67 (MQ=255) aTCCTGGCTTATTAACACTAAAACCACCCCCCCACCGCGGGGGGCCGGGGGGGAAGCGTCGACAGTg < 1:35625066/67‑1 (MQ=255) aTCCTGGATTATTAACATTAAAATCACCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:5244311/67‑1 (MQ=255) gtcTTATAAACATAAAAAACAACCGCCCACCGGGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:25507797/66‑1 (MQ=255) ggattattaACACAAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:3423129/65‑1 (MQ=255) ggCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:14117724/68‑1 (MQ=255) ggCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:17568445/68‑1 (MQ=255) ggCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:22879174/68‑1 (MQ=255) ggCTTATTAACAGGAAAATCACCCGCCCAACGCGTTGGGCCGGTGGGGAAGCGTCGACAGTGCCAGCa < 1:978251/68‑1 (MQ=255) ggCTTATAAACATTAAAATCCCCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:26812313/68‑1 (MQ=255) gCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa > 1:26354330/1‑67 (MQ=255) cTTATTAACAGTAAAATCAACCGCCAAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc > 1:27321123/1‑68 (MQ=255) ttattaACATTAAAATCAACCGCCCAACGCGTGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc < 1:36749972/67‑1 (MQ=255) ttattaACAGTAAAATCAACCCCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc < 1:22605831/67‑1 (MQ=255) ttattaACACTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc < 1:4875628/67‑1 (MQ=255) ttataaACAGAAAAATCAACCCCCCACCGCGGTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCa < 1:30730851/68‑1 (MQ=255) tattaAAAGTAAAATCAACCGCCCAACGCGGTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAt < 1:19062987/68‑1 (MQ=255) tataaACAGAAAAATCACCCCCCCACCGCGTGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAt < 1:32968805/68‑1 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:12674631/1‑66 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:25915903/1‑66 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:29667118/1‑66 (MQ=255) taACAGTAAAAACAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGccaccaccaat > 1:33765826/1‑64 (MQ=255) aaCAGTAAAATCAACCGCCCAGCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:20445608/1‑65 (MQ=255) aaCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:23913519/1‑65 (MQ=255) aCAGTAAAATCAACCGCCCAACGCGTTGGGCggttgattttactggtattaagccaggattccgg > 1:12821801‑M1/1‑31 (MQ=255) aCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTg > 1:30262736/1‑68 (MQ=255) aCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTg > 1:20007556/1‑68 (MQ=255) aGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTgg > 1:655935/1‑67 (MQ=255) aTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc > 1:29057413/1‑68 (MQ=255) aTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc > 1:33943993/1‑68 (MQ=255) aaCCGCCCAACGCGTTGGGCCGGGGGGGAGCCGTCGAAAGTGCCAGCACCATTACGGGCCCCTCCGc > 1:31319102/1‑67 (MQ=255) aCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc > 1:6200422/1‑67 (MQ=255) aCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc > 1:36437326/1‑67 (MQ=255) aCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc > 1:15925340/1‑67 (MQ=255) aCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCa < 1:29341756/68‑1 (MQ=255) | GAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCA > CP009273/999526‑999657 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |