| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 999,598 | A→C | 6.6% | T66P (ACG→CCG) | ycbF → | putative periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 999,598 | 0 | A | C | 6.6% | 1869.4 / 16.7 | 703 | T66P (ACG→CCG) | ycbF | putative periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base A (477/168); new base C (2/53); total (481/222) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.01e-26 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 706 total aligned reads displayed.
TATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCT > CP009273/999533‑999665 | atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGC < 1:43262294‑M1/68‑1 (MQ=255) atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGC < 1:28454690‑M1/68‑1 (MQ=255) ttttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAAGAGTAAAATCAACCGCCCAACGCGTTGc > 1:21234164‑M1/1‑67 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑g > 1:41769498/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑g > 1:12487924/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑g > 1:12683730/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑g > 1:28189839/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑g > 1:33899094/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑g > 1:33076809/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑g > 1:32140628/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑ggg > 1:36349322/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑ggg > 1:42466032/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑ggg < 1:19687571/68‑1 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑ggg > 1:2619789/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑ggg > 1:9300707/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑ggg < 1:4278907/68‑1 (MQ=255) gACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑gggg > 1:25440753/1‑68 (MQ=255) cTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑gggggg > 1:15211650/1‑68 (MQ=255) cTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑gggggg > 1:25672995/1‑68 (MQ=255) cTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑gggggg > 1:32966780/1‑68 (MQ=255) cTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑gggggg > 1:7845019/1‑68 (MQ=255) cTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑gggggg > 1:37925042/1‑68 (MQ=255) tcccACTCAGGAATCCTGGCTTATAAACAGAAAAATCCACCGCCCACCGCGTTGGGCC‑GGGGGGGAAg < 1:15982938/66‑1 (MQ=255) tACCAGTCAGGAATCCTGTCTTATAAACACAAAAAACAACCCCCCAACGCGTTGGGCC‑GGGGGGGAAg < 1:40660792/68‑1 (MQ=255) tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCACCCGCCCAACGCGTTGGGCC‑GGGGGGGAAg < 1:7594523/68‑1 (MQ=255) tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAg < 1:29378467/68‑1 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCCACGCGTTGGGCC‑GGGGGGGAAGc > 1:10105968/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGc > 1:26534135/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGc > 1:40217333/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGc > 1:10635970/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGc > 1:3852511/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGc > 1:37220548/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGc > 1:2411158/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGc > 1:1503364/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGc > 1:23354784/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGc > 1:6142143/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGc > 1:17754399/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGc > 1:6722483/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGc > 1:7217027/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGc > 1:13566151/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGc > 1:8231311/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGc > 1:18034590/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAAACAACAGCCCAACGCGTGGGGCC‑GGGGGGGAAGc > 1:1568505/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGGAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGc > 1:41259897/1‑68 (MQ=255) ccAGTCAGGAATCCTGTCTTATTAACACAAAAATCCACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCg < 1:5915647/68‑1 (MQ=255) cAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGCGCC‑GGGGGGGAAGCg > 1:14644199/1‑67 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCg > 1:3422828/1‑67 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCg > 1:3827780/1‑67 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCg > 1:19348899/1‑67 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCg > 1:17443893/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAACTCACCCGCCCAACGCGTTGGGCggttgattttactgttaataag < 1:20768803‑M1/67‑23 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTg < 1:27680009/67‑1 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTg > 1:33736580/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGGCGGGCC‑GGGGGGGAAGCGTCGACAGTg < 1:28734300/67‑1 (MQ=255) aTCCTGGCTTATTAACACTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTg < 1:22261554/67‑1 (MQ=255) aTCCTGGCTTATAAACAGTAAAATCACCCGCCCACCGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTg < 1:35823718/67‑1 (MQ=255) ccTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGGAAGCGTCGACAGTGcc > 1:29660431/1‑68 (MQ=255) ccTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCa > 1:18439155/1‑68 (MQ=255) ccTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCa > 1:27390646/1‑68 (MQ=255) ccTGGATTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTgaaa > 1:30496618/1‑65 (MQ=255) tGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGc > 1:7483354/1‑68 (MQ=255) ggattattaACAGAAAAATCACCCGCCCACCGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCa < 1:24935040/65‑1 (MQ=255) ggCTTATTACCAGTAAAATCACCCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCa < 1:11683776/68‑1 (MQ=255) ggCTTATTAACATTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCa < 1:14247825/68‑1 (MQ=255) ggCTTATTAACAGTAAAATCAACCGCCCACCGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCa < 1:31632950/68‑1 (MQ=255) ggCTTATTAACAGGAAAATCACCCGCCCAACGCGTTGGGCC‑GGTGGGGAAGCGTCGACAGTGCCAGCa < 1:978251/68‑1 (MQ=255) gCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCa > 1:35469764/1‑67 (MQ=255) cTTATTAACAGTAAAATCCCCCGCCCACCGCGGGGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCAcc < 1:27143812/68‑1 (MQ=255) cTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCAcc > 1:13940363/1‑68 (MQ=255) cTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCAcc > 1:29067217/1‑68 (MQ=255) ttattaACAGTAAAATCAACCCCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCAcc < 1:22605831/67‑1 (MQ=255) ttattaACACTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCAcc < 1:4875628/67‑1 (MQ=255) ttattaAAAGTAAAATCAACCCCCCACCGGGTGGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCAcc < 1:26900681/67‑1 (MQ=255) tattaACAGTAAAATCACCCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCAt < 1:30974610/68‑1 (MQ=255) tattaACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCAt < 1:39216805/68‑1 (MQ=255) tattaACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCAt < 1:2149130/68‑1 (MQ=255) tattaAAAGTAAAATCAACCGCCCAACGCGGTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCAt < 1:19062987/68‑1 (MQ=255) attaACATTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCAtt < 1:37618908/68‑1 (MQ=255) ataaaCAGTAAAATCAACCGCCCACCGCGTGGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCAtt < 1:5257641/65‑1 (MQ=255) ataaaCAGTAAAATCAACCGCCCAACGCGTGGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCAtt < 1:11982030/65‑1 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:16604515/1‑66 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:32681297/1‑66 (MQ=255) aaCAGTAAAATCAACCGCCCAGCGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:20445608/1‑65 (MQ=255) aaCAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:24493007/1‑65 (MQ=255) aCAGTAAAATCAACCGCCCAACGCGTTGGGCggttgattttactggtattaagccaggattccgg > 1:12821801‑M1/1‑31 (MQ=255) aCAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTg > 1:20007556/1‑68 (MQ=255) aCAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTg > 1:11171249/1‑68 (MQ=255) aCAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTg > 1:38938838/1‑68 (MQ=255) aCAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTg > 1:35030459/1‑68 (MQ=255) aGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTgg > 1:8800432/1‑67 (MQ=255) aaaTCACCCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCt < 1:30018912/68‑1 (MQ=255) aTCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc > 1:39741242/1‑68 (MQ=255) aTCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc > 1:35179061/1‑68 (MQ=255) aCCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc > 1:22942835/1‑67 (MQ=255) aCCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc > 1:4443966/1‑67 (MQ=255) aCCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc > 1:36748358/1‑67 (MQ=255) gCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACtt > 1:4207980/1‑68 (MQ=255) ccccAACGCGGTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACtt < 1:641579/67‑1 (MQ=255) ccAACGCGTTGGGCggttgattttactgttaataagccaggattcctgactggtattagtcagtaaa < 1:566487‑M1/67‑54 (MQ=255) ccAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAt < 1:34487290/68‑1 (MQ=255) | TATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCC‑GGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCT > CP009273/999533‑999665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |