| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,665,749 | T→G | 5.1% | L115R (CTG→CGG) | hcaD → | phenylpropionate dioxygenase, ferredoxin reductase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,665,749 | 0 | T | G | 5.1% | 1326.3 / 2.3 | 414 | L115R (CTG→CGG) | hcaD | phenylpropionate dioxygenase, ferredoxin reductase subunit |
| Reads supporting (aligned to +/- strand): ref base T (83/301); new base G (25/2); total (111/303) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.04e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 426 total aligned reads displayed.
TAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTG > CP009273/2665683‑2665816 | tAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCt < 1:3515438/67‑1 (MQ=255) aaCCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGc < 1:22459949/68‑1 (MQ=255) tccATCGGCGAATGCTGTCACTGGGTTCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCGCTGcc < 1:32176790/67‑1 (MQ=255) ccAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCGCGCCCCCTGCCg > 1:40518016/1‑68 (MQ=255) ccAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGGCCGCTGCCg > 1:33441963/1‑68 (MQ=255) ccAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGCCCGCTGGCg > 1:3539029/1‑68 (MQ=255) ccAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGCCCGCTGCCg > 1:39778220/1‑68 (MQ=255) ccAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGCCCGCGGCCg > 1:19524872/1‑68 (MQ=255) ccAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGc > 1:17802724/1‑66 (MQ=255) ccAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCg > 1:13398478/1‑68 (MQ=255) ccAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCg > 1:30226518/1‑68 (MQ=255) ccAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCg > 1:29218016/1‑68 (MQ=255) ccAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCGGCCg > 1:26677115/1‑68 (MQ=255) cGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCTCTGCCGTTGc < 1:33036601/68‑1 (MQ=255) cGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGc < 1:23060507/68‑1 (MQ=255) cGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGc < 1:24061136/68‑1 (MQ=255) cGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGc < 1:29877980/68‑1 (MQ=255) cGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGc < 1:1647122/68‑1 (MQ=255) cGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGc < 1:686409/68‑1 (MQ=255) cGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGc < 1:13653805/68‑1 (MQ=255) cGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGc < 1:4114323/68‑1 (MQ=255) ggCGAAAGCTGGCACTGGGAGCAGCTTTTTATAGCAACCGGCGCGGCACCTAGACCCCAGCCGATgga > 1:20687439/1‑66 (MQ=255) cGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCtt < 1:2843185/67‑1 (MQ=255) cGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCtt < 1:15362990/67‑1 (MQ=255) aaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATg < 1:15625560/68‑1 (MQ=255) aaGCTGGCACTGGGAGCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATg < 1:35973966/68‑1 (MQ=255) aGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGc < 1:18010857/68‑1 (MQ=255) aGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCACCTCGACCCCGGCCCTTGGGTGATGc > 1:33138387/1‑68 (MQ=255) tGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCa < 1:17344136/66‑1 (MQ=255) tGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCa < 1:14214178/66‑1 (MQ=255) tGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCa < 1:38435214/66‑1 (MQ=255) tGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACt < 1:3654060/68‑1 (MQ=255) tGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACt < 1:36861827/68‑1 (MQ=255) tGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACt < 1:18157601/68‑1 (MQ=255) tGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACt < 1:10781187/68‑1 (MQ=255) tGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACt < 1:26876632/68‑1 (MQ=255) tGGCACTGGGATCAGCGTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACt < 1:9608519/68‑1 (MQ=255) tGGCACGGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGGTGCTTGATGCa < 1:12878342/66‑1 (MQ=255) ggCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTg < 1:19022451/68‑1 (MQ=255) aCTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTggg < 1:22707107/67‑1 (MQ=255) aCTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGga > 1:3239602/1‑68 (MQ=255) aCTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCCTTGCTTGATGCACTGGga > 1:14386697/1‑68 (MQ=255) aCTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCGCTGCCGTTGCTTGATGCACTGGga > 1:8457575/1‑68 (MQ=255) aCTGGGATCAGCTTCTTATAGCAACCGGCGCGGCCGCTCGCCCGCGTCCCTTGCTTGATTCACAgggc > 1:34027160/1‑67 (MQ=255) ggATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAAc > 1:931577/1‑68 (MQ=255) gATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGCCCGCTGCCGTTGCTTGATGCCCTGGGAGAAc > 1:1657675/1‑67 (MQ=255) gATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAAc > 1:31044321/1‑67 (MQ=255) tCAGCTTTTTTTAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACgct < 1:29551034/68‑1 (MQ=255) tCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACgct < 1:33637071/68‑1 (MQ=255) tCAGCTTTTTATACCAACCTGCGGGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGTGAACgct < 1:36301006/68‑1 (MQ=255) cAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACgctg < 1:37586263/68‑1 (MQ=255) gCTTTTTATAGCAACCGGCGCGGCAGCTCGCCCGCTGCCGTTGCTTGATGCACTGGGAGCACgcggcg > 1:1576711/1‑67 (MQ=255) gCTTTTTATAGCAACCGGCGCGGCAGCTCGCCCGCTGCCGGTGCTTGATTCCCTGGGGGAACgctgct > 1:38174370/1‑68 (MQ=255) tttttATAGCAACCGGCGCGGCAGCTCGACCGCTGGCGGTGCTTGCTGCACTGGGACAACGCTGCttt > 1:24581394/1‑68 (MQ=255) tttttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACgctgc < 1:10910026/65‑1 (MQ=255) tttttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACgctgc < 1:40854126/65‑1 (MQ=255) tttttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACgctgc < 1:6510159/65‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCAGTGGGAGAACGCTGCTTTAc < 1:41223812/68‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAc < 1:41845291/68‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAc < 1:4000170/68‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAc < 1:41657200/68‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAc < 1:5129676/68‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAc < 1:14972149/68‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAc < 1:13823310/68‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAc < 1:42979980/68‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAc < 1:4912493/68‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAc < 1:20113487/68‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAc < 1:20249976/68‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAc < 1:3467536/68‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAc < 1:2540463/68‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAc < 1:32847201/68‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAc < 1:12195539/68‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAc < 1:27946713/68‑1 (MQ=255) tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAc < 1:3137554/68‑1 (MQ=255) ttATAGCAACCGGCGCGGCAGCTCGACCGCTTCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAcc < 1:18686566/68‑1 (MQ=255) ttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAcc < 1:43623309/68‑1 (MQ=255) ttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTAcc < 1:889849/68‑1 (MQ=255) aGCAACCGGCGCGGCCGCGCGCCCGCTGCCGTTGCTTGATGCACGGGGTGAACGCTGGTTTACCCTa > 1:5169289/1‑67 (MQ=255) aGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTAc < 1:43211042/68‑1 (MQ=255) aGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTAc < 1:34400531/68‑1 (MQ=255) gCAACCGGCGCGGCAGCTCGGCCGCTGCCGTTGCTTGATGCACGGGGAGAACGCTGCGTTACCCTAc > 1:20350720/1‑67 (MQ=255) gCAACCGGCGCGGCAGCTCGCCCGCTGCCGTTGGTTGATGCACTGGGGGAACGCGGCTTTTCCCTAc > 1:37148613/1‑67 (MQ=255) aaCCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACg < 1:40387684/66‑1 (MQ=255) aaCCGGCGCGGCAGCTCGACCGCTGCCGGTGGGTGATGCACTGGGAGAAAGCTGGATTACCCTACGcc > 1:25706595/1‑68 (MQ=255) aaCCGGCGCGGCAGCGCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGc < 1:42246902/67‑1 (MQ=255) ccGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCAt < 1:28850174/68‑1 (MQ=255) ggCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGc < 1:21582918/68‑1 (MQ=255) ggCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGc < 1:43537847/68‑1 (MQ=255) cAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGa < 1:39467346/67‑1 (MQ=255) cAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGAt > 1:39181948/1‑68 (MQ=255) cgcGACCGCTGCCGTTGCTTGTTGCACTGGGACAACGCTGCTTTACCCTACGCCAAGCCGGTGATgcc < 1:21259766/66‑1 (MQ=255) cTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATgcc > 1:10288472/1‑68 (MQ=255) tCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATgcc < 1:38711292/67‑1 (MQ=255) tCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATgcc < 1:6164913/67‑1 (MQ=255) tCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATgcc < 1:25049809/67‑1 (MQ=255) tCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATgcc < 1:11813013/67‑1 (MQ=255) tCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATgcc < 1:10029873/67‑1 (MQ=255) cGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGa < 1:7796739/68‑1 (MQ=255) tGCCGTTGCTTGTTGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTg < 1:27600750/68‑1 (MQ=255) tGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTg < 1:30640489/68‑1 (MQ=255) | TAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTG > CP009273/2665683‑2665816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |