| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,711,673 | A→C | 8.0% | V72G (GTT→GGT) | yfiF ← | putative methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,711,673 | 0 | A | C | 8.0% | 947.1 / 12.1 | 341 | V72G (GTT→GGT) | yfiF | putative methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (234/75); new base C (3/29); total (237/104) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 350 total aligned reads displayed.
AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGG > CP009273/2711607‑2711740 | aaaCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAAc < 1:20171540/68‑1 (MQ=255)aaaCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAAc < 1:6446153/68‑1 (MQ=255)aaaCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAAc < 1:7544212/68‑1 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGTCGCGCGGGAAACCg > 1:33392923/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAACCCg > 1:31722908/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg > 1:5158971/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg > 1:605139/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg > 1:34861181/1‑68 (MQ=255) ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt > 1:34047895/1‑67 (MQ=255) aCCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑Gcc > 1:28725771/1‑68 (MQ=255) ccACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑Gcc > 1:7947279/1‑67 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑Gcccc > 1:35473188/1‑66 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCAc > 1:21178151/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCAc > 1:10348288/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCAc > 1:37843016/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCAc > 1:38162042/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCAc > 1:3887818/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCAc > 1:26751670/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCAc > 1:10624012/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCAc > 1:10454973/1‑68 (MQ=255) cACTGATACCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCAc > 1:40443613/1‑68 (MQ=255) cACGGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCAc > 1:20526994/1‑68 (MQ=255) aCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACg > 1:14582231/1‑68 (MQ=255) ttGCCCCGGTGACCGGCTTTTGCCGGGGTGGAACCCCCGGGCGCGGGGTAAACGGTGC‑GCCACGGCGa < 1:35299499/67‑1 (MQ=255) aTGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGa > 1:13205426/1‑68 (MQ=255) tGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGaa > 1:8464387/1‑68 (MQ=255) tGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGaa > 1:1124155/1‑68 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGaa > 1:26073628/1‑66 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGaa > 1:2830533/1‑66 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGaa > 1:28240449/1‑66 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGaa > 1:3761969/1‑66 (MQ=255) aCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCt > 1:41863029/1‑67 (MQ=255) ccGTGATGGGCCTTTCCCGGCGTCTCACCCCCCGGCGCGCGGTAACCCGTGC‑GCCACGGCGAATCTTc < 1:284699/68‑1 (MQ=255) ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCtt > 1:19939449/1‑67 (MQ=255) ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCtt > 1:33158331/1‑67 (MQ=255) ccGTGAGCGGCCTTTGCCGGCGGCTCACCCCCCGGCGCGCGGGAAACCGGGC‑GCCACGGCGAATCTTc < 1:32012269/68‑1 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTc > 1:34241185/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTc > 1:30953752/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTc > 1:29421660/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTc > 1:40867167/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTc > 1:12117779/1‑67 (MQ=255) gTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTccc > 1:41573421/1‑68 (MQ=255) gTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTccc > 1:30079967/1‑68 (MQ=255) ctgggCATTTGCCGGCGTCGCAACACCCGGCGCGGGTGAACCGGGGC‑GCCACGGCGAATCTTCCCACt < 1:39512043/65‑1 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACtt > 1:20391626/1‑68 (MQ=255) cGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACtt > 1:42745168/1‑67 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCg > 1:42886151/1‑68 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCg > 1:30346959/1‑68 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCg > 1:34674046/1‑68 (MQ=255) ccTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCgg > 1:21010910/1‑68 (MQ=255) cTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCgg > 1:5423687/1‑67 (MQ=255) cTTTTCCGGCGTCACATCACCCGGCGCGCGGGAAACCGTGC‑GCAACGGCGAATCTTCCCACTTGCgg > 1:11509184/1‑67 (MQ=255) ttttCCGGCGACCCATCCCCCGGCGCGCGGAAACCCGGGC‑GCCACGGCGAATCTTCCCACTTGCGGTc < 1:13529169/68‑1 (MQ=255) ccGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:29795702/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:8750573/1‑68 (MQ=255) cGGGGTCTCATCCCGCGGGGCGGGGGAACCCTTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACggg < 1:41147079/68‑1 (MQ=255) ggggTCTCATCACCCGGCGCGCGGCAACCCGGGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:22044206/65‑1 (MQ=255) ggggTCTAATCCCCCGGCGCGCGGTAAACCGGGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:12219327/65‑1 (MQ=255) ggCGTCTCATCCCCCGGCGCGCGGGAAAGCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:37135642/68‑1 (MQ=255) gggTCTCATCCCCCGGGGCGCGGTAACCCGGGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:5654750/65‑1 (MQ=255) cGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCCCggg > 1:15498793/1‑65 (MQ=255) gTCTCAACCCCCGGCGCGCGGTAAACCGGGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCt < 1:8929538/67‑1 (MQ=255) gTCTAATCCCCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCt < 1:23998940/67‑1 (MQ=255) tctcATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTg > 1:22290327/1‑67 (MQ=255) ctcATCCCCGGGCGGGGGGTAACCCGGGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc < 1:9098014/68‑1 (MQ=255) ctcATCACCCGGCGCGCGGGAACCCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa < 1:10008315/67‑1 (MQ=255) ctcATC‑CCCGGCCGGGGGGTAACCGGGGGGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa < 1:23276129/67‑1 (MQ=255) ctcAGCCCCCGGGGCGCGGTAAACGGGGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa < 1:13794346/67‑1 (MQ=255) tcatcaCCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc > 1:18851445/1‑67 (MQ=255) atccCCGGGCGCGCGGTAAACGGGGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct < 1:37910901/68‑1 (MQ=255) atcaCCCGGCGCGCGGGAAACCGTGC‑GCCATGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct < 1:17567225/68‑1 (MQ=255) accaCCCGGCGCGGGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct < 1:2534173/66‑1 (MQ=255) accaCCCGGCGCGCGGGAAACGGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct < 1:4882880/66‑1 (MQ=255) tcaCCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc > 1:43380637/1‑64 (MQ=255) cccccGGCGCGGGGGAACCCGGGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt < 1:1667135/66‑1 (MQ=255) cccccGGCGCGCGGTAACCGGGGC‑CCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt < 1:37967721/66‑1 (MQ=255) caCCCGGCGGGGGGTAACCGGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt < 1:31102463/68‑1 (MQ=255) aCCCGGCGCGCGGGAAACCGTGA‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt > 1:2439521/1‑68 (MQ=255) cccGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:15060926/1‑68 (MQ=255) cccGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:13412916/1‑68 (MQ=255) cccGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:16413753/1‑68 (MQ=255) cccGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:39679323/1‑68 (MQ=255) cccGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:6966882/1‑68 (MQ=255) cccGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:23543963/1‑68 (MQ=255) cccGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:19172026/1‑68 (MQ=255) cccGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:19424719/1‑68 (MQ=255) cccGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:19914050/1‑68 (MQ=255) ccGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:8162894/1‑68 (MQ=255) ccGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:9575197/1‑68 (MQ=255) ccGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:27875291/1‑68 (MQ=255) ccGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:1714896/1‑68 (MQ=255) ccGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:27284174/1‑68 (MQ=255) cGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:37318142/1‑67 (MQ=255) cGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGGTTg > 1:3839269/1‑67 (MQ=255) cgcgcgGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCa > 1:12782037/1‑67 (MQ=255) cgcgcgGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCa > 1:2695717/1‑67 (MQ=255) gcgcgGTAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCAt < 1:36297150/67‑1 (MQ=255) gcgcgGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTAGCGGTCACGGGGCTGACTCTGTTTGTCATc > 1:18373823/1‑68 (MQ=255) gcgcgGGAAACCGTGC‑GCCACGGCGAATCTCCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATc > 1:36606146/1‑68 (MQ=255) aaaCCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcg > 1:21607/1‑68 (MQ=255) | AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGC‑GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGG > CP009273/2711607‑2711740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |