| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,263,466 | G→T | 16.2% | intergenic (+505/+109) | yhaC → / ← rnpB | pentapetide repeats‑related protein/RNase P, M1 RNA component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,263,466 | 0 | G | T | 16.2% | 896.2 / 32.4 | 363 | intergenic (+505/+109) | yhaC/rnpB | pentapetide repeats‑related protein/RNase P, M1 RNA component |
| Reads supporting (aligned to +/- strand): ref base G (197/80); new base T (32/31); total (238/125) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.76e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 364 total aligned reads displayed.
GTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCT > CP009273/3263400‑3263533 | cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaa < 1:6615691/67‑1 (MQ=35) attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaa > 1:26410529/1‑66 (MQ=35) tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTg > 1:32718208/1‑68 (MQ=35) tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTg > 1:34913613/1‑68 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTgc > 1:2330862/1‑66 (MQ=25) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:37832663/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:30655335/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:33038431/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:15472870/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:18943689/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:35386099/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:13792196/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:28676120/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:12590314/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:38415312/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:39766728/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:21445013/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAc < 1:12622023/68‑1 (MQ=35) tcagtttcacctggtttacgtAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGGGAAactgccc < 1:19265232‑M1/47‑4 (MQ=255) tCTTTCTGGCACTCCAAAATCTAAAACCCGCCGTAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc < 1:2084543/68‑1 (MQ=11) tCTTTCTGCCCCTCCTATAGCTAATACGCGCCTAAGCGTGTTTTTACGTAAACCAGGTTAAACTGAcc < 1:11921510/68‑1 (MQ=11) tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc > 1:43251568/1‑68 (MQ=35) tCTTTCTGCCACTCCAAAAGCAAAAACCCGCCGTAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc < 1:40687767/68‑1 (MQ=255) cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAATGACCg > 1:21945347/1‑68 (MQ=25) cTGCCCCTCCAAACTCTAAAAGTGGCCTATGAGTTTTTTTAATTATACATGGTGAAACTGACCGATaa < 1:10631762/68‑1 (MQ=255) tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATaa > 1:8801860/1‑67 (MQ=35) gacccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc < 1:34287978/66‑1 (MQ=25) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg > 1:26231144/1‑67 (MQ=35) ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc > 1:17956468/1‑68 (MQ=35) ccacTCAAAAATCATAAACCCGCCGTAACGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc < 1:36222247/64‑1 (MQ=11) cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc > 1:18450872/1‑67 (MQ=35) ctggtttacgtAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc < 1:11481404‑M1/55‑1 (MQ=255) ccTCCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGc < 1:535941/68‑1 (MQ=255) ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGc < 1:29433257/68‑1 (MQ=37) acTCCACAATCACAAACCCGCCGCAGCGGTTTTTTACGTAAACCCGGTTAAACTGACCGATAAGCCGc < 1:12257859/67‑1 (MQ=11) acTCAAAAATCAAAAACCCGCCGTACCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGc < 1:22979172/67‑1 (MQ=11) cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttc < 1:31980619/68‑1 (MQ=40) cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttc < 1:10013234/68‑1 (MQ=40) cAAAAGCAAAAACCAGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGgtt > 1:30095039/1‑66 (MQ=255) ttacgtaaaaacccgcttcggcgggttttaACGAAAACCAGGTGAAACTGACCGATAAGCCGctttct < 1:16580718‑M2/38‑1 (MQ=255) aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttct < 1:8002328/68‑1 (MQ=255) aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttct < 1:15000124/68‑1 (MQ=255) aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttct < 1:25626207/68‑1 (MQ=255) aaaGCAAAAACCCGCCGAAGCGGGTTTTTAGGT‑AACCAGGTTAAACTGACCGATAAGCCGctttcttt < 1:21765217/68‑1 (MQ=14) aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt < 1:5061472/68‑1 (MQ=40) aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt > 1:38112331/1‑68 (MQ=40) aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt < 1:3657108/68‑1 (MQ=40) aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt < 1:13715601/68‑1 (MQ=40) aGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGcgttctttg > 1:7515232/1‑67 (MQ=255) aGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt > 1:2055534/1‑68 (MQ=40) aGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAACCAGGTGAACCTGACCGATAAGCCGCTTTCgttt > 1:1083924/1‑68 (MQ=14) gCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg > 1:25234335/1‑68 (MQ=39) gCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg > 1:39413445/1‑68 (MQ=39) tAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg < 1:32334840‑M1/67‑1 (MQ=255) catAAACCCGCCGCATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTgg > 1:22648033‑M1/4‑68 (MQ=255) ttAAACCCGCCGCACCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTgg > 1:20946531‑M1/3‑67 (MQ=255) AAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg > 1:27813958‑M1/1‑67 (MQ=255) tAAACCCGCCGTACCGGGTTTTTACGTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:22849945‑M1/2‑68 (MQ=255) tAAACCCGCCGTACCGGGTTATTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:42943521‑M1/2‑68 (MQ=255) tAAACCCGCCGCATCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:4559817‑M1/2‑68 (MQ=255) cAAACCCGCCGTATCGGGTTTTTACGTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:23984075‑M1/2‑68 (MQ=255) cAAACCCGCCGTATCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:25063857‑M1/2‑68 (MQ=255) AAAACCCGCCGTATCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:12865829‑M1/1‑68 (MQ=255) AAAACCCGCCGTACCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:37053095‑M1/1‑68 (MQ=255) AAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:3028910‑M1/1‑68 (MQ=255) AAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:29880584‑M1/1‑68 (MQ=255) AAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:35870729‑M1/1‑68 (MQ=255) AACCCGCCGCATCGGGTTTTTACGTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGt > 1:13996195‑M1/1‑66 (MQ=255) ACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACAGACCGATAACCCGCTTTCTTTTGGGtata < 1:11628484‑M1/68‑1 (MQ=255) CCCGCCGCACCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTT‑GGGTATAGc > 1:31559066‑M1/1‑68 (MQ=255) CCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGtata < 1:19877390‑M1/67‑1 (MQ=255) CCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGTCCGATAAGCCGCTTTCTTTTGGGTATAGc > 1:15245503‑M1/1‑68 (MQ=255) CCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGc > 1:15765492‑M1/1‑68 (MQ=255) CCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGc > 1:24735908‑M1/1‑68 (MQ=255) CCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGc > 1:26959556‑M1/1‑68 (MQ=255) GCCGAAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGt > 1:13323853‑M1/1‑68 (MQ=255) GCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGt > 1:40181237‑M1/1‑68 (MQ=255) GCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGt > 1:26763907‑M1/1‑68 (MQ=255) GCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGt > 1:43023316‑M1/1‑68 (MQ=255) GCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGt > 1:23139201‑M1/1‑68 (MQ=255) gAAGCGGGTTTTTACGTACACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt < 1:1951247/68‑1 (MQ=255) gAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGcgtcgt < 1:18649585/68‑1 (MQ=255) tttttACGTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTc < 1:8876932‑M2/63‑1 (MQ=255) tttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGt < 1:614954‑M2/62‑1 (MQ=255) ttattACGTACACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTc < 1:20275437‑M2/63‑1 (MQ=255) ttattACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGt < 1:33983176‑M2/62‑1 (MQ=255) cGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGtcattcat > 1:22261085/1‑68 (MQ=255) gTAAATCTGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGGCGTGGACAGTCATTCATc < 1:41640929/68‑1 (MQ=11) gTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATc < 1:9524526/68‑1 (MQ=255) gTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATc < 1:16772002/68‑1 (MQ=255) gTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattcata > 1:5657126/1‑67 (MQ=255) aCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCtt > 1:17617007/1‑66 (MQ=39) ccAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCtttat > 1:37374747/1‑66 (MQ=37) ccAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATAtt > 1:1975999/1‑65 (MQ=255) cAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCttttt > 1:42764641/1‑65 (MQ=255) cAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCt > 1:33810667/1‑67 (MQ=35) tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCAATCAAATTTCGGccc > 1:27088145/1‑67 (MQ=11) tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCCACTTTCTGcccc > 1:1130785/1‑68 (MQ=255) tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATCATTCTGccca > 1:38753840/1‑67 (MQ=11) tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCATCATTCTGcg > 1:1043437/1‑65 (MQ=11) | GTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCT > CP009273/3263400‑3263533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |