| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,400 | A→G | 5.7% | S271S (AGT→AGC) | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,400 | 0 | A | G | 5.7% | 1776.4 / 36.6 | 555 | S271S (AGT→AGC) | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base A (283/227); new base G (21/11); total (316/239) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 560 total aligned reads displayed.
AGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAA > CP009273/3462336‑3462467 | aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:22510116/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:25541118/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:1092190/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:42808033/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:36988469/1‑68 (MQ=255)aGCCAGGGTGACCATCCTGGCTGTTTTGTTTTGCCCTTGACGCTCGATCTTCACGCCATGGGTTACt < 1:28949952/67‑1 (MQ=255) ccAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGc > 1:39848914/1‑68 (MQ=255) ccAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGc > 1:35881702/1‑68 (MQ=255) cAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTgg < 1:33558531/66‑1 (MQ=255) gggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATc > 1:31057167/1‑68 (MQ=255) gggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATc > 1:37181258/1‑68 (MQ=255) ggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCg > 1:4750829/1‑68 (MQ=255) ggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCg > 1:39249659/1‑68 (MQ=255) gTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCTCGAACATAACGCCATGGGTTGCTGGCATc < 1:38985814/66‑1 (MQ=255) gTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt > 1:16799628/1‑68 (MQ=255) gTTACCATACTGGCTGATTTCTGTTGCCGTAGCGGCTCGAACATAACGCCATGGGTTGCTGGCATc < 1:4050008/66‑1 (MQ=14) ttACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt < 1:30229493/67‑1 (MQ=255) ttACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt < 1:24206884/67‑1 (MQ=255) ccATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGttt > 1:440312/1‑66 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGttttgt > 1:14057550/1‑66 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:38050821/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:34650325/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:43472448/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:2764621/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:9542085/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct < 1:20959519/68‑1 (MQ=255) aTACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:2780931/1‑68 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:32862437/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:8122165/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:11938961/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:40261228/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCCTtttctt > 1:27304057/1‑67 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctttc > 1:14834243/1‑68 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctttc > 1:1224604/1‑68 (MQ=255) cTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctttc > 1:10202302/1‑67 (MQ=255) cTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCg < 1:42629317/68‑1 (MQ=255) cTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCg < 1:520957/68‑1 (MQ=255) cTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCg < 1:38265398/68‑1 (MQ=255) tGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTTCTGGCATCATTGTCTTTCGc > 1:40948997/1‑68 (MQ=18) tGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATca > 1:29744016/1‑56 (MQ=255) tGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGc > 1:33316498/1‑68 (MQ=255) tGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGc > 1:18674037/1‑68 (MQ=255) ggCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCa > 1:38423195/1‑68 (MQ=255) cTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATc > 1:19965476/1‑68 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATACCGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:6264264/68‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:19459652/68‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:22147275/68‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:36494738/68‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:19280560/68‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:24745468/68‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:17644417/68‑1 (MQ=255) tttCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACCGGCATCGTTTTCTTTCGCATCGcc < 1:20438005/67‑1 (MQ=255) tCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTgg > 1:41364264/1‑68 (MQ=255) tCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTgg > 1:28733955/1‑68 (MQ=255) tCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTgg < 1:43082684/68‑1 (MQ=255) tCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTgg < 1:17307674/68‑1 (MQ=255) tCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTgg < 1:35392676/68‑1 (MQ=255) tGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGc < 1:26401507/68‑1 (MQ=255) tGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGc < 1:5566274/68‑1 (MQ=255) cgttgccgCGCGAACATAACGCCATGGGTTGCTGGCATCGTTTGTTTTCGCATCGCCAGGGCAGTTTg > 1:18302721/1‑68 (MQ=17) gccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgc > 1:24974831/1‑68 (MQ=255) cgcgcgAACATACCGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:29049074/67‑1 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTGTTTCGCATCACCTGGGCAATTAGCagcg > 1:42287845/1‑67 (MQ=21) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:36050872/67‑1 (MQ=255) cgcgcgAACAATACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTAGCcgcg < 1:10778365/67‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:37477857/68‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:8599402/68‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:10009556/68‑1 (MQ=255) gcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCt < 1:18054881/67‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:12753820/68‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:17066485/68‑1 (MQ=255) tcATAACGCCATGGGTTACTGGCATCTTTTTCTTTCGCATCGCCTGGGCTATTTGCCGCGCATACcca < 1:14332976/67‑1 (MQ=37) aCATAACGCCATGGTTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCGTAccc < 1:21310456/67‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAccc < 1:3938848/67‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:16435275/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:69968/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:22897059/68‑1 (MQ=255) tAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACccacca < 1:34254817/68‑1 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:21811648/1‑68 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:28239354/1‑68 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc < 1:41703316/68‑1 (MQ=255) cGCCAGGGGTTACTGGCAGCGGTTTCTTTCGCATCGCCTGGGCCAGTTGCCGCGCCTCCCCCCCAGGc > 1:6394351/1‑68 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGACTGGGCAATTTGCCGCGCCTACCCACCAGGCg > 1:20699167/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:15441047/1‑68 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGtt < 1:36370241/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:33028198/68‑1 (MQ=255) cATGGGTTACTGGCATCGTCTTCTATCGCACCGCGTGGGCAATTTTCCGCGCCTACCCACCAGGCGt < 1:7391766/67‑1 (MQ=255) cATGGGTTACTGGCATCGTCTGCTATCGCATCGCCTGGGCAATGTGCCGCGCCTACCCACCAGGCGtt < 1:25846584/68‑1 (MQ=255) aTGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:30718804/1‑66 (MQ=255) tGGGTTACTGGCTTCTTTATCTATCGCACCCCCTGGGCAATCTTCCGAGCCTACCCACCAGGCGTTCt < 1:23367753/68‑1 (MQ=16) tGGGTTACTGGCATCGTTTTCTTTGGAATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:31461812/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:9235911/68‑1 (MQ=255) gggTTACTGGCTTCTTTTTCTTTGGCATCGCCTGGGCAATTTGAAGCGCCTACCCACCAGGCGTTCtt < 1:3778320/68‑1 (MQ=37) gggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTtttt > 1:11311910/1‑68 (MQ=255) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta > 1:35003777/1‑68 (MQ=255) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta > 1:1162419/1‑68 (MQ=255) gTTACTGGAATCGTTTTCTTTCGCACCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTta < 1:25208571/67‑1 (MQ=255) ttACTGGCATCGTTTTCTTTCGCATCGCCAGGGCAATTTGCAGCGCCTACCCACCAGGCGTTTTtata > 1:26839461/1‑68 (MQ=37) aCTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATaaa > 1:13564183/1‑68 (MQ=255) aCTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCAGCGCCTACCCACCAGGCGTTCTTATaaa > 1:31943726/1‑68 (MQ=255) | AGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAA > CP009273/3462336‑3462467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |