| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,438 | C→A | 6.6% | A259S (GCA→TCA) | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,438 | 0 | C | A | 6.6% | 2464.5 / 20.9 | 824 | A259S (GCA→TCA) | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base C (168/568); new base A (26/34); total (194/630) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.44e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 826 total aligned reads displayed.
TGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCT > CP009273/3462373‑3462505 | cgcgcgAACATACCGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:29049074/67‑1 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTGTTTCGCATCACCTGGGCAATTAGCagcg > 1:42287845/1‑67 (MQ=21) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg > 1:10893255/1‑67 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:37477857/68‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:29919709/68‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:15388263/68‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:11705516/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:69968/68‑1 (MQ=255) aTAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACccacc > 1:20201797/1‑68 (MQ=255) gCCATGGTTTACTGGCATCGTTTGATTTCGCATCGCCAGGGCAATTAGAAGCGCCTACCCACCAGGca > 1:10301958/1‑67 (MQ=17) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg > 1:9555903/1‑68 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg < 1:3896951/68‑1 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCTCCTGGGCAATTTGAAGCCGCTACCCACCAGGcat > 1:28280025/1‑66 (MQ=18) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:6308565/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:29553574/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTATTTCGCATCGCCAGGGCAATTAGAAGCGCCTACCCACCAGGcat > 1:42670353/1‑66 (MQ=18) cATGGGTTACTGGCATCGTTTTCTTTCGGATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:38107605/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGtt < 1:33557517/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:25762648/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTGCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:36069209/68‑1 (MQ=255) aTGGGTTACTGGCATCGTTTTGTTTCGCATCGCCAGGGCAATTTGAAGAGGCTACCCACCAGGCAtta > 1:14367998/1‑67 (MQ=14) gggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTtttt > 1:11311910/1‑68 (MQ=255) gggTTACTGGCATCGTTTTCTTTCGCAGCCCCAGGGCAATTTGCAGCGCCTACCCACCAGGCATtttt > 1:18538208/1‑68 (MQ=21) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta > 1:35003799/1‑68 (MQ=255) gTTACTGGAATCGTTTTCTTTCGCACCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTta < 1:25208571/67‑1 (MQ=255) ttACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTtata > 1:32341711/1‑68 (MQ=255) ttACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTtata > 1:20951671/1‑68 (MQ=255) aCTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCAGCGCCTACCCACCAGGCGTTCTTATaaa > 1:31943726/1‑68 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTGTTATaaaa > 1:22456425/1‑66 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTGTTATAAAt > 1:32818330/1‑67 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTGTTATAAAt > 1:37184919/1‑67 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAAt > 1:23986945/1‑67 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCATTCTTATAAAtt > 1:27092729/1‑67 (MQ=255) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCTTTATTATaaaac > 1:40153391/1‑65 (MQ=255) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTAGCCGCGCCTACCCACCAGGCGTTGTTatatatc > 1:9291778/1‑66 (MQ=38) ggCATCGTTTTCTTTAGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTATTATaaaag > 1:40850833/1‑65 (MQ=255) gCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTATTatatatcac > 1:40331108/1‑65 (MQ=38) tCGTTTTCTTTGGCATCGCCTGGGCAATTTGCAGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:33212679/68‑1 (MQ=255) ctttttCTTTCGCATCGCGTGGGCAATTTTCAGCGCCTACCCACCAGGCGTTCTTATAAATGccgccg < 1:30414850/66‑1 (MQ=37) cGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGccgccg < 1:41379078/68‑1 (MQ=255) cGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGccgccg < 1:2258577/68‑1 (MQ=255) cGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGccgccg > 1:19693533/1‑68 (MQ=255) tttttCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:12845171/67‑1 (MQ=255) ttgttctatcGGATCGCCTGGGCAATTTTACGCGCCTACCCACCAGTCGTTCTTATAAATGCCGCCGt < 1:38417400/65‑1 (MQ=255) ttcttcgttcgTATCGCCTGGGCAATTTTACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:19350995/65‑1 (MQ=37) gtgttctttgGGACCGCGTGGGCAATTTTTCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:39227559/65‑1 (MQ=21) gTTTTCTTTCGCATCGCGTGGGCAATATGCCGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:20500268/68‑1 (MQ=38) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:1797540/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:12549908/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:819409/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:3119679/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:21609205/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:26569427/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTCGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:3768091/68‑1 (MQ=255) gTTTTCTATCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:2301526/68‑1 (MQ=255) gTTTGCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:5084866/68‑1 (MQ=255) gTTTGCTTTCGCATCGCCTGGGCAATTTGCCGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:31492892/68‑1 (MQ=255) ttttCTTTCGCAGCGCGTGGGCAATTTTTAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:36847372/67‑1 (MQ=25) ttctttcGTAGCGCGTGGGCAATTTGTCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:39817788/68‑1 (MQ=37) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:8731936/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:17160448/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:770121/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:35770741/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:27435899/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:18131654/68‑1 (MQ=255) ctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAg < 1:7889323/68‑1 (MQ=255) ttggCATCGCCTGGGCAATTTGTAGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:13257786/65‑1 (MQ=37) ttcGCATCGCGTGGGCAATTTGTAGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:24349884/68‑1 (MQ=25) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:6700144/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:41749890/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:36406836/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:14787553/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:24550450/68‑1 (MQ=255) tcGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:4725488/67‑1 (MQ=255) gCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAAc < 1:14133617/68‑1 (MQ=255) gCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAAc > 1:17471872/1‑68 (MQ=255) gCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAAc < 1:22171995/68‑1 (MQ=255) aacGCCTGGGCAATATGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:23431049/66‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:28745136/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:35427308/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCGTATAAATGCCGCCGTTATAGATAACGt > 1:34386359/1‑68 (MQ=255) aTCGCCTGGGCAATTTGCAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:27804743/68‑1 (MQ=255) gCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAcc < 1:13757246/68‑1 (MQ=255) gCCTGGGCAATTTGACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAcc < 1:22862477/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:16130275/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:33948729/68‑1 (MQ=255) gggCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGt < 1:30821475/68‑1 (MQ=255) gggCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGt < 1:21925220/68‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATTACGTACCAGGTag > 1:1000192/1‑68 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag > 1:166501/1‑68 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag > 1:17778963/1‑68 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:4438301/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:5519838/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:24862796/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:7319892/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:20742499/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGACGCCGTTATATATAACGTCCCATGTag > 1:13593398/1‑68 (MQ=39) cAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTaga < 1:21141153/68‑1 (MQ=255) cAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag < 1:34758252/67‑1 (MQ=255) aaTTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTagaa > 1:42994651/1‑68 (MQ=255) | TGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCT > CP009273/3462373‑3462505 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |