| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,172,502 | G→T | 8.9% | D444Y (GAT→TAT) | rpoB → | RNA polymerase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,172,502 | 0 | G | T | 8.9% | 1872.9 / 127.3 | 557 | D444Y (GAT→TAT) | rpoB | RNA polymerase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (305/201); new base T (27/23); total (333/224) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 608 total aligned reads displayed.
AAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGT > CP009273/4172436‑4172569 | aaaGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCgatga > 1:36537337/1‑68 (MQ=255)aaaGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCgatga < 1:28640345/68‑1 (MQ=255) aaGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCgatgat < 1:20239910/68‑1 (MQ=255) aaGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCgatgat < 1:13837297/68‑1 (MQ=255) aaGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCgatgat < 1:30036150/68‑1 (MQ=255) gacgacATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGata > 1:25634949/1‑67 (MQ=255) cgacATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGatat < 1:27894287/66‑1 (MQ=255) cgacATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCg > 1:23086383/1‑68 (MQ=255) gacATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGa < 1:33173503/68‑1 (MQ=255) gacATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGa < 1:15743934/68‑1 (MQ=255) gacATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGa < 1:5636267/68‑1 (MQ=255) acATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATTATATCGa > 1:13631206/1‑67 (MQ=255) tcatTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGacca > 1:27466939/1‑67 (MQ=255) tcatTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGacca > 1:42720441/1‑67 (MQ=255) tcatTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGacca > 1:24922318/1‑67 (MQ=255) tcatTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGacac > 1:30585056/1‑65 (MQ=255) tcatTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGacaa > 1:30274877/1‑65 (MQ=255) catTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGaccacc < 1:9119203/68‑1 (MQ=255) catTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGaccacc < 1:13128929/68‑1 (MQ=255) atTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGaccacc > 1:21517635/1‑67 (MQ=255) atTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCt < 1:26399510/68‑1 (MQ=255) atTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCt < 1:38582953/68‑1 (MQ=255) atTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCt < 1:8187725/68‑1 (MQ=255) atTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCt < 1:8440942/68‑1 (MQ=255) atTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCt < 1:376511/68‑1 (MQ=255) atTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCt < 1:3693369/68‑1 (MQ=255) atTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCt < 1:34407994/68‑1 (MQ=255) tGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCg < 1:3832753/68‑1 (MQ=255) tGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCg > 1:10432334/1‑68 (MQ=255) tGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCg > 1:12489187/1‑68 (MQ=255) tGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCg < 1:40323239/68‑1 (MQ=255) tGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCa > 1:26593125/1‑68 (MQ=255) tGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCa > 1:7156485/1‑68 (MQ=255) tGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCa > 1:41102748/1‑68 (MQ=255) tGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCa > 1:12649394/1‑68 (MQ=255) tGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCa > 1:15273927/1‑68 (MQ=255) tGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCa > 1:24020852/1‑68 (MQ=255) gTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCa > 1:33384107/1‑67 (MQ=255) gTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCa > 1:17225269/1‑67 (MQ=255) gTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCa > 1:3142727/1‑67 (MQ=255) tATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAAcc > 1:29011444/1‑68 (MQ=255) aTGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcg < 1:1414025/68‑1 (MQ=255) aTGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcg < 1:27180850/68‑1 (MQ=255) aTGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcg < 1:16496733/68‑1 (MQ=255) aTGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcg < 1:14793497/68‑1 (MQ=255) aTGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcg > 1:5129374/1‑68 (MQ=255) tGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATTATATCGACCACCTCGGCAACcgt < 1:17596347/68‑1 (MQ=255) tGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcgt < 1:4186389/68‑1 (MQ=255) tGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcgt < 1:11193549/68‑1 (MQ=255) tGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcgt < 1:4711821/68‑1 (MQ=255) tGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcgt < 1:22313052/68‑1 (MQ=255) tGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcgt < 1:21204259/68‑1 (MQ=255) tGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcgt < 1:39390908/68‑1 (MQ=255) tGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcgt < 1:7464250/68‑1 (MQ=255) tGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcgt < 1:10080504/68‑1 (MQ=255) tGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcgt < 1:37422212/68‑1 (MQ=255) aaaaaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcgtcg > 1:35772412/1‑68 (MQ=255) aaaaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcgtcg < 1:32178307/67‑1 (MQ=255) aaaGCTCATCGATATCCGTAACTGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTa < 1:43525889/68‑1 (MQ=255) aaaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTa < 1:16059784/68‑1 (MQ=255) aaaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTa < 1:18041021/68‑1 (MQ=255) aGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATTATATCGACCACCTCGGCAACCGTCGTAt > 1:12341760/1‑67 (MQ=255) aGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTAt > 1:28278631/1‑67 (MQ=255) aGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTAt > 1:41347504/1‑67 (MQ=255) aGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTAt > 1:35426776/1‑67 (MQ=255) tCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTAtccgttcc < 1:31792908/68‑1 (MQ=255) gATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTAtccgttccgt < 1:16856683/68‑1 (MQ=255) gATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTAtccgttccgt > 1:19842235/1‑68 (MQ=255) atCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTg > 1:22751738/1‑67 (MQ=255) atCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTg > 1:15113428/1‑67 (MQ=255) atCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGACGTATCCGTTCCGTTg > 1:11984789/1‑67 (MQ=255) tCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGc > 1:34734574/1‑68 (MQ=255) ccGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCg > 1:7792815/1‑68 (MQ=255) ccGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCg > 1:29879342/1‑68 (MQ=255) tAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGaaa > 1:40887985/1‑68 (MQ=255) aCGGTAAAGGCGAAGTCGATTATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATg > 1:2074462/1‑68 (MQ=255) aCGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATg > 1:18279159/1‑68 (MQ=255) aCGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATg > 1:6682396/1‑68 (MQ=255) aCGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATg > 1:6246844/1‑68 (MQ=255) ggTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGc > 1:600363/1‑68 (MQ=255) tAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCg < 1:34992394/67‑1 (MQ=255) ggCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaaa > 1:2163174/1‑67 (MQ=255) ggCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaaa > 1:18946323/1‑67 (MQ=255) ggCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaaa > 1:36078080/1‑67 (MQ=255) ggCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaaa > 1:36303830/1‑67 (MQ=255) ggCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaaa > 1:42425181/1‑67 (MQ=255) ggCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaaa > 1:40087152/1‑67 (MQ=255) gCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaaaa > 1:25196213/1‑67 (MQ=255) gCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaaaa > 1:235749/1‑67 (MQ=255) cGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAAcc > 1:32689671/1‑68 (MQ=255) cGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAAcc > 1:1161027/1‑68 (MQ=255) cGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAAcc > 1:34166844/1‑68 (MQ=255) cGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTACCCGTTCCGTTGGCGAAATGGCGGaaaa < 1:18532114/66‑1 (MQ=255) aGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGt < 1:33720155/68‑1 (MQ=255) tCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGtt > 1:23243330/1‑67 (MQ=255) tCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGtt > 1:26134197/1‑67 (MQ=255) tgatATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCcgcg > 1:1100076/1‑68 (MQ=255) tgatATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCcgcg > 1:43090893/1‑68 (MQ=255) tgatATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCcgc < 1:1935651/67‑1 (MQ=255) tgatATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCcgc < 1:24539520/67‑1 (MQ=255) | AAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGT > CP009273/4172436‑4172569 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |