| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,172,748 | C→T | 76.9% | H526Y (CAC→TAC) | rpoB → | RNA polymerase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,172,748 | 0 | C | T | 76.9% | 1340.1 / inf | 615 | H526Y (CAC→TAC) | rpoB | RNA polymerase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base C (85/57); new base T (301/171); total (386/229) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01 | |||||||||||
Only 100 of 666 total aligned reads displayed.
AGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTC > CP009273/4172681‑4172814 | agagTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGc > 1:25649834/1‑68 (MQ=255) agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGtac > 1:10264887/1‑68 (MQ=255) agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGtac > 1:22360257/1‑68 (MQ=255) agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGtac > 1:31268724/1‑68 (MQ=255) agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGtac > 1:37896832/1‑68 (MQ=255) agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGtac > 1:40455851/1‑68 (MQ=255) agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGtac > 1:8795641/1‑68 (MQ=255) gTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGtaca > 1:29805968/1‑68 (MQ=255) gTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGca < 1:27748237/66‑1 (MQ=255) ttcttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACaa > 1:38150079/1‑68 (MQ=255) tcttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACaaa < 1:14667198/68‑1 (MQ=255) ttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAAGAACCCGCTGTCTGAGATTACGTACAAAcg < 1:39416171/68‑1 (MQ=255) ttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAAcg < 1:41309666/68‑1 (MQ=255) ttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAAcg < 1:1421348/68‑1 (MQ=255) tcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAAcg > 1:43567326/1‑67 (MQ=255) tcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAAcg > 1:7422055/1‑67 (MQ=255) cGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAAcgtc < 1:9530174/68‑1 (MQ=255) ggTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAAcgtcg > 1:19320660/1‑68 (MQ=255) tCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTa > 1:18914558/1‑67 (MQ=255) tCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTa > 1:23546530/1‑67 (MQ=255) tCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTa > 1:35315978/1‑67 (MQ=255) ccagccagCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTAtc < 1:11311170/68‑1 (MQ=255) ccagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTcc > 1:320180/1‑67 (MQ=255) ccagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTcc > 1:391469/1‑67 (MQ=255) ccagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTcc > 1:13881192/1‑67 (MQ=255) ccagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTcc > 1:22803255/1‑67 (MQ=255) cagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTAtctc < 1:36259915/65‑1 (MQ=255) cagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTAtctc < 1:13210615/65‑1 (MQ=255) cagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTAtctc < 1:26710721/65‑1 (MQ=255) tGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCAc < 1:37430393/66‑1 (MQ=255) tGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCAc < 1:36953293/66‑1 (MQ=255) tGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTc > 1:1218166/1‑68 (MQ=255) tGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTc > 1:2530913/1‑68 (MQ=255) tGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTc > 1:36478242/1‑68 (MQ=255) gTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCg > 1:7910560/1‑68 (MQ=255) ctcAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCgg < 1:3478974/67‑1 (MQ=255) ctcAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCgg < 1:8382627/67‑1 (MQ=255) ctcAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCgg < 1:40191759/67‑1 (MQ=255) ctcAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCgg < 1:18169879/67‑1 (MQ=255) ctcAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCgg < 1:43344145/67‑1 (MQ=255) ctcAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCgg < 1:10943022/67‑1 (MQ=255) ctcAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGc < 1:27030893/68‑1 (MQ=255) cAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGccc > 1:4291088/1‑68 (MQ=255) ttATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCGGCACTCGGCCCAGGc > 1:18589155/1‑68 (MQ=255) ttATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCa < 1:2833990/65‑1 (MQ=255) aTGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg < 1:41712755/68‑1 (MQ=255) aTGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg < 1:24993600/68‑1 (MQ=255) aTGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg < 1:20239938/68‑1 (MQ=255) aTGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg < 1:40919206/68‑1 (MQ=255) tGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg > 1:27391583/1‑67 (MQ=255) tGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg > 1:34388251/1‑67 (MQ=255) tGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGt < 1:16719384/68‑1 (MQ=255) ggACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTc < 1:33729036/68‑1 (MQ=255) gACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg > 1:17079735/1‑65 (MQ=255) gACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg > 1:16049688/1‑65 (MQ=255) gACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg > 1:41985748/1‑65 (MQ=255) ccAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCCGGCGGTCTg > 1:4976/1‑67 (MQ=255) ccAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTg > 1:4835420/1‑67 (MQ=255) ccAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTg > 1:19975036/1‑67 (MQ=255) ccAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGa < 1:39856888/68‑1 (MQ=255) cAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGAc < 1:6780273/68‑1 (MQ=255) cAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGAc < 1:25879054/68‑1 (MQ=255) acaacCCGCTGTCTGAGATTACGTACAACCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGAcc < 1:10061386/65‑1 (MQ=255) acaacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGt > 1:7568651/1‑68 (MQ=255) acaacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt > 1:9265972/1‑68 (MQ=255) acaacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt > 1:5785908/1‑68 (MQ=255) acaacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt > 1:10519314/1‑68 (MQ=255) acaacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt > 1:5167/1‑68 (MQ=255) acaacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt > 1:6588020/1‑68 (MQ=255) acaacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCg < 1:19659478/67‑1 (MQ=255) acaacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt < 1:29596694/68‑1 (MQ=255) acaacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt > 1:20789203/1‑68 (MQ=255) ccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACg > 1:42391799/1‑68 (MQ=255) ccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACg > 1:33169530/1‑68 (MQ=255) ccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACg > 1:29107069/1‑68 (MQ=255) ccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACg > 1:41089219/1‑68 (MQ=255) ccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACg > 1:1646881/1‑68 (MQ=255) ccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACg > 1:36706191/1‑68 (MQ=255) tGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCa > 1:27927928/1‑68 (MQ=255) cTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGc < 1:21188132/68‑1 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:1762997/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:21896061/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:5588474/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:6097929/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:21604739/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:24103931/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:24246343/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:26146099/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:11882900/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:30347140/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:12799361/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:15158901/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:38647991/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:33456145/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:35019394/1‑68 (MQ=255) aTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:3177635/1‑68 (MQ=255) aTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:35822833/1‑68 (MQ=255) ttACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGaa < 1:32459230/68‑1 (MQ=255) tACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAg > 1:15698249/1‑68 (MQ=255) gCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTc > 1:24656870/1‑68 (MQ=255) | AGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTC > CP009273/4172681‑4172814 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |