BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	691,928	G→A	5.5%	noncoding (33/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	691,928	0	G	A	5.5%	1460.6 / 37.6	465	noncoding (33/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base G (118/312); new base A (2/23); total (122/343)
Fisher's exact test for biased strand distribution p-value = 3.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 468 total aligned reads displayed.

TCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAA  >  CP009273/691862‑691992

tCGCCGAATCGTGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGa                                                                 <  1:17877740/68‑1 (MQ=255)

tCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGa                                                                 <  1:25932569/68‑1 (MQ=255)

  tccGAACCGAGCTTTTTTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                                                               <  1:12208449/67‑1 (MQ=17)

  gCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                                                               <  1:9739825/68‑1 (MQ=255)

  gCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                                                               <  1:28743603/68‑1 (MQ=255)

  gCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                                                               <  1:14294873/68‑1 (MQ=255)

   ccGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                                                               >  1:4547765/1‑67 (MQ=255)

    cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATc                                                              <  1:7388285/67‑1 (MQ=255)

    cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATc                                                              <  1:30767094/67‑1 (MQ=255)

       aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                          <  1:29623619/68‑1 (MQ=255)

       aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                          <  1:495416/68‑1 (MQ=255)

       aGCGAGCTTTTTTAATGTGGCGGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                          <  1:38373136/68‑1 (MQ=255)

        gCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                          >  1:11388855/1‑67 (MQ=255)

         cGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                        <  1:33775589/68‑1 (MQ=255)

         cGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                        <  1:12895002/68‑1 (MQ=255)

          tatcTTTTTTAATTTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGc                                                        <  1:6829922/64‑1 (MQ=14)

          gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                         <  1:40813595/66‑1 (MQ=255)

          gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                         <  1:40436466/66‑1 (MQ=255)

          gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                       <  1:39567936/68‑1 (MQ=255)

          gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                       <  1:42233446/68‑1 (MQ=255)

          gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                        <  1:17034831/67‑1 (MQ=255)

          gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                        <  1:19429590/67‑1 (MQ=255)

          gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                        <  1:39799915/67‑1 (MQ=255)

          gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                        <  1:34024729/67‑1 (MQ=255)

          gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                        <  1:30463457/67‑1 (MQ=255)

          gAGCGTTTTTAAGCTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGcc                                                       <  1:13375464/68‑1 (MQ=14)

           cgCTTTATTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                      <  1:774741/67‑1 (MQ=255)

            gCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGACtt                                                     >  1:32409983/1‑68 (MQ=255)

              ttttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAc                                                   <  1:24759419/68‑1 (MQ=255)

               tttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                  <  1:24490614/68‑1 (MQ=255)

               tgattAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                  <  1:31714290/65‑1 (MQ=0)

                ttttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                          <  1:39232938/59‑1 (MQ=255)

                 tttAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                                <  1:27354670/68‑1 (MQ=18)

                 tttAATGTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGc                                                <  1:9453356/68‑1 (MQ=11)

                  ttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                               <  1:15727039/68‑1 (MQ=40)

                  ttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                               <  1:36343367/68‑1 (MQ=40)

                  ttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                               >  1:32002107/1‑68 (MQ=40)

                    aaTGTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCACAAACCGGTGCCTTACCGCTTg                                             <  1:26748622/68‑1 (MQ=11)

                     aTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg                                            <  1:23473074/68‑1 (MQ=35)

                     aTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg                                            <  1:43084687/68‑1 (MQ=35)

                     aTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg                                            <  1:17560617/68‑1 (MQ=35)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTa                                                    >  1:2024762/1‑59 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCtttc                                            >  1:4178683/1‑65 (MQ=18)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:6008813/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:29733853/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           <  1:35172600/68‑1 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:36126474/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           <  1:38632699/68‑1 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:37248547/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:23919024/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:41407895/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:20707948/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:21272339/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           <  1:21768912/68‑1 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:22082286/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:10838209/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:16592762/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:15042292/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           <  1:24154417/68‑1 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:41925693/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           <  1:26584755/68‑1 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:27075905/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:42628917/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:5615056/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           <  1:12428178/68‑1 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTCGc                                           >  1:10083281/1‑68 (MQ=18)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAACCGGTGCCTTACCGCTTGGc                                           >  1:13652818/1‑68 (MQ=18)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGACTGAATAAGAAAACGGAGCCTTACCGCTTGGc                                           >  1:23163126/1‑68 (MQ=11)

                      tgtgGCTGGGGTACGAGGATTCGAACCTAGGAATGCCGGAATCAGAATCCGGTGCCTTACAGCTTGGc                                           <  1:33468173/68‑1 (MQ=11)

                      agtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           <  1:27766926/67‑1 (MQ=14)

                                   cGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTg                              <  1:35736084/68‑1 (MQ=33)

                                   cGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTg                              <  1:17758173/68‑1 (MQ=33)

                                    gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaaaaa                              >  1:36788096/1‑64 (MQ=1)

                                     aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaataa                              >  1:1118886/1‑63 (MQ=1)

                                     aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaagaa                              >  1:26071285/1‑63 (MQ=1)

                                     aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACtcat                            >  1:16170679/1‑65 (MQ=14)

                                      ggATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTggg                            <  1:37067385/67‑1 (MQ=37)

                                       gATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTggtt                           >  1:18936637/1‑65 (MQ=25)

                                         ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                        <  1:31272354/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:42881209/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:10524493/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:8608614/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:13921804/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:40181889/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:14569950/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:18374044/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:19573243/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:25358357/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:33004618/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:34766347/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:37979214/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:41042348/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGGGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:22352668/68‑1 (MQ=255)

                                               ccTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACa                  <  1:9070562/68‑1 (MQ=255)

                                                 tCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg                <  1:28073360/68‑1 (MQ=255)

                                                  cGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAgg               <  1:14098739/68‑1 (MQ=255)

                                                   ggAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGt              <  1:22623320/68‑1 (MQ=255)

                                                              aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa   <  1:11948821/68‑1 (MQ=255)

                                                              aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa   <  1:5413332/68‑1 (MQ=255)

                                                              aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa   <  1:6586924/68‑1 (MQ=255)

TCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAA  >  CP009273/691862‑691992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`