BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	692,040	G→A	5.1%	noncoding (33/75 nt)	glnV ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	692,040	0	G	A	5.1%	1224.3 / 27.1	414	noncoding (33/75 nt)	glnV	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base G (275/110); new base A (11/10); total (288/126)
Fisher's exact test for biased strand distribution p-value = 8.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 420 total aligned reads displayed.

TACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTC  >  CP009273/691973‑692104

tACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAg                                                                  >  1:13295531/1‑68 (MQ=255)

  cAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                >  1:26447620/1‑68 (MQ=255)

  cAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                >  1:25743210/1‑68 (MQ=255)

   aaGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                >  1:1641873/1‑67 (MQ=255)

   aaGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                >  1:5775371/1‑67 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCaaaaac                                                              >  1:28090784/1‑65 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:25996051/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:42054477/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:38263140/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:2423081/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:24592623/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:25474104/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:9584575/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:27348201/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:27672542/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:28421648/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:33961545/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:29754681/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:31806507/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:14492226/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:5095820/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:15181369/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:43515635/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:11453594/1‑68 (MQ=255)

       tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                           >  1:36087824/1‑68 (MQ=255)

       tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                           >  1:35282300/1‑68 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                         >  1:19818015/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                         >  1:11360250/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                         >  1:1928415/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                         >  1:18987304/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                         >  1:17546620/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAAAATCCGGTg                                                         >  1:3040045/1‑66 (MQ=255)

            gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGcc                                                       <  1:82679/67‑1 (MQ=17)

            gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                      >  1:21917602/1‑68 (MQ=255)

             tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                     >  1:40521054/1‑68 (MQ=255)

             tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCAGGAATCAGAATCCGGTGACtt                                                     >  1:42822979/1‑68 (MQ=255)

             tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAACGCCGGAATCAGAATCCGGTGCCtt                                                     >  1:1302091/1‑68 (MQ=255)

                 taaTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCg                                                 <  1:3649823/67‑1 (MQ=255)

                  taTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGc                                                <  1:7354901/67‑1 (MQ=11)

                  taTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                                <  1:38990155/67‑1 (MQ=255)

                  aaTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                                <  1:11072086/68‑1 (MQ=255)

                   aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                               >  1:22772956/1‑68 (MQ=40)

                   aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                               >  1:14007877/1‑68 (MQ=40)

                   aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                               >  1:36812208/1‑68 (MQ=40)

                   aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                               >  1:26632962/1‑68 (MQ=40)

                      aaTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg                                            <  1:43137497/68‑1 (MQ=35)

                      aaTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg                                            <  1:7635249/68‑1 (MQ=35)

                       tttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCATAAACCGGTGCCTTACCGCTTGGc                                           <  1:39622149/67‑1 (MQ=11)

                       aTTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCATAATCCGGTGCCTTACCGCTTGGc                                           <  1:39276887/68‑1 (MQ=11)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                          >  1:6481697/1‑68 (MQ=31)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                          >  1:37878104/1‑68 (MQ=31)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                          >  1:42384851/1‑68 (MQ=31)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCAGGTGCCTTACCGCTTGGCg                                          >  1:8804433/1‑68 (MQ=14)

                                    cGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacaa                              <  1:28771399/68‑1 (MQ=33)

                                     gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGTCTTACCGCTTGGCGATACCCcaacagt                             >  1:41961942/1‑66 (MQ=9)

                                     gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACaaa                             <  1:11645119/68‑1 (MQ=35)

                                      aGGATTCGAAGCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                            >  1:21265650/1‑68 (MQ=255)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacatag                            >  1:34582982/1‑65 (MQ=14)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacaggg                            >  1:33841030/1‑65 (MQ=1)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacagag                            >  1:6183763/1‑65 (MQ=14)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacaagt                            >  1:5915512/1‑66 (MQ=18)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACaaaa                            >  1:37633820/1‑67 (MQ=25)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                            >  1:25092212/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                            >  1:20870170/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                            >  1:14256851/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                            >  1:12367770/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                            >  1:10503283/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                            >  1:9126512/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                            >  1:32506562/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                            >  1:30661748/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                            >  1:10325897/1‑68 (MQ=37)

                                        gATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTg                          >  1:10023382/1‑68 (MQ=40)

                                        gATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTg                          >  1:32055505/1‑68 (MQ=40)

                                          ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                        <  1:38645608/68‑1 (MQ=255)

                                              aaCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACACATTGCATTTg                    >  1:37359188/1‑68 (MQ=11)

                                               aCCTCGGAATGCCGGAATCAGAATCCGGTGGCTTAAAGCGTGGACATACCCCAACAAATTTGTGTTGa                   >  1:19469205/1‑68 (MQ=14)

                                               aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGa                   >  1:34716917/1‑68 (MQ=255)

                                                ccTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGaa                  >  1:18290289/1‑68 (MQ=255)

                                                   cGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAAttt               <  1:33034266/68‑1 (MQ=255)

                                                    ggAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTg              <  1:20372856/68‑1 (MQ=255)

                                                      aaTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGc             <  1:31348898/67‑1 (MQ=255)

                                                      aaTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGc             <  1:36677273/67‑1 (MQ=255)

                                                       aTGCCGGAATCAGAATCCGGTGCCTTACCTCTGGGGGATACCCCAACAAATTGGGTATGAATTTGACg           >  1:12249212/1‑68 (MQ=17)

                                                        tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTAATTGGTTTTGAATTTGCCGa          <  1:535253/68‑1 (MQ=255)

                                                         gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGaa         <  1:17841783/68‑1 (MQ=255)

                                                          ccGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAAc        >  1:6888304/1‑68 (MQ=255)

                                                          ccGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAAc        >  1:11826992/1‑68 (MQ=255)

                                                           gggAATCAGAATCAGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAAc        <  1:23502604/66‑1 (MQ=255)

                                                              aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGc             <  1:13460763/59‑1 (MQ=255)

                                                              aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGACGAACatat    >  1:15444336/1‑68 (MQ=255)

                                                               aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat    <  1:17168763/67‑1 (MQ=255)

                                                               aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat    <  1:14913782/67‑1 (MQ=255)

                                                               aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat    <  1:37123975/67‑1 (MQ=255)

                                                               aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat    <  1:22303733/67‑1 (MQ=255)

                                                               aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat    <  1:21565892/67‑1 (MQ=255)

                                                               aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat    <  1:41639096/67‑1 (MQ=255)

                                                               aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat    <  1:31153542/67‑1 (MQ=255)

                                                               aTCAGAATCCCGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat    <  1:18759999/67‑1 (MQ=255)

                                                                tCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTc  >  1:41301553/1‑68 (MQ=255)

                                                                tCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGATTTGAAGTTGAAGCACATAtta  >  1:27184851/1‑67 (MQ=17)

TACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTC  >  CP009273/691973‑692104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`