BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	692,313	G→T	5.1%	intergenic (‑27/+8)	glnW ← / ← glnU	tRNA‑Gln/tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	692,313	0	G	T	5.1%	1114.7 / 2.2	357	intergenic (‑27/+8)	glnW/glnU	tRNA‑Gln/tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base G (222/108); new base T (15/11); total (238/119)
Fisher's exact test for biased strand distribution p-value = 3.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 386 total aligned reads displayed.

CGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACC  >  CP009273/692246‑692379

cGGTATCAAAAACCGGTGCCTTACCGCTTAGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCg                                                                    <  1:34297917/68‑1 (MQ=255)

    aTCACAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaaga                                                                <  1:1674200/68‑1 (MQ=255)

    aTCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaaga                                                                <  1:18269620/68‑1 (MQ=255)

    aTCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaaga                                                                <  1:10389059/68‑1 (MQ=255)

    aTCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaaga                                                                <  1:24583190/68‑1 (MQ=255)

       acaaaCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAagaaga                                                             <  1:946599/66‑1 (MQ=255)

         aatccGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATg                                                           <  1:8427703/65‑1 (MQ=255)

         aaaCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATg                                                           <  1:35201408/68‑1 (MQ=255)

         aaaCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATg                                                           <  1:31949186/68‑1 (MQ=255)

          aaCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATgg                                                          <  1:33120355/68‑1 (MQ=255)

           aCCGGTGCCTTACCGCTTGGCGATACCCCAATACCCCGGCGGTGACCCGCGTAAGGGAAGAACATg                                                           >  1:3977185/1‑66 (MQ=12)

           aCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACGCGTAGAAGATg                                                           >  1:37181674/1‑66 (MQ=255)

            ccGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCTAAGAAGATGGCt                                                        <  1:11822370/68‑1 (MQ=255)

              ggTGCCTTACCGCGTGGCGCTACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTgg                                                      <  1:40313164/68‑1 (MQ=255)

                tGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTggg                                                     <  1:1469588/67‑1 (MQ=255)

                 gCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGt                                                   <  1:1972152/68‑1 (MQ=255)

                  ccTTACCGCTTGGCGATACCCCAATACACGGGCGGTGAACCGGGTAGGCTAAGAAGATGGCTGGGGt                                                   >  1:15253871/1‑67 (MQ=21)

                  ccTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCg                                                                    <  1:20319105/50‑1 (MQ=255)

                   cTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAATAAGATGGCTGGGGTAc                                                 <  1:10272577/68‑1 (MQ=255)

                   cTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTAc                                                 <  1:25756702/68‑1 (MQ=255)

                   cTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTAc                                                 <  1:21515525/68‑1 (MQ=255)

                    gtACCGCTTGGCCATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTAcc                                                <  1:4143682/67‑1 (MQ=255)

                      tccGCTTGGCGATACCCCAATAACCGGGCGGAGAACCGCTTACTCGAAGAAGATGGCTGGGGTACGTg                                              >  1:23934076/2‑68 (MQ=39)

                      aCCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTg                                              >  1:40071194/1‑68 (MQ=255)

                      aCCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTAGAAGCAGCTGGCTGGGGTAcat                                               >  1:31151507/1‑65 (MQ=38)

                        cGCTTGGCGATACCCCAATTACCGGGCGGTGAAGAGATTACTCGAAGACGATGGCTGGGGTACCTGGa                                            <  1:4604034/68‑1 (MQ=37)

                        cGCTTGGCGATACCCCAATAGCCGGGCGGTGACCCGGTTACTCGAAGAAGATGGCTGGGGTACCTg                                              >  1:15676856/1‑66 (MQ=255)

                        cGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGa                                            >  1:27602300/1‑68 (MQ=255)

                        cGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGa                                            <  1:3062587/68‑1 (MQ=255)

                        cGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGa                                            >  1:38891364/1‑68 (MQ=255)

                        cGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGa                                            >  1:36016771/1‑68 (MQ=255)

                        cGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGa                                            >  1:35679231/1‑68 (MQ=255)

                        cGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGa                                            <  1:35475502/68‑1 (MQ=255)

                        cGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGa                                            >  1:23515798/1‑68 (MQ=255)

                        cGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCGTACTCGAAGAAGATCGCTGGGGTACCTGGa                                            >  1:26430125/1‑68 (MQ=255)

                        cGCTTGGCGATACCCCAAAAACCGGGCGGTGAACCGCTTAATCTAAGAAGATGGCTGGTGTACCTCGa                                            >  1:18703995/1‑68 (MQ=255)

                        agCTTGGCGATACCCCAATAACCTGTCTGTGAACCGCTTACTCGAATAATATGGCTGGGGTACCTGGa                                            <  1:17594865/67‑1 (MQ=255)

                         gCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGATGGGGTACCTGGAt                                           >  1:9315772/1‑68 (MQ=255)

                         gCTTGGCGATACCCCAATAACCGCGCGGTGAACCGCTTACTAGAACAAGATGGCTTGGGTACCTGGAt                                           >  1:15482652/1‑68 (MQ=255)

                         gCTTGGCGATACCCCAAAACCCCAGCGGTGAACCGGGTAGTCGAAGAAGCTCGCTACTGTACCTCGAt                                           >  1:18470454/1‑68 (MQ=255)

                         gCTTGGCGATACCCCAAAAACCGGGCGGTGAACCGCTTAATCGAAGAAGATCGCTCCGGTACCTCGa                                            >  1:25182000/1‑67 (MQ=255)

                          cTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGAt                                           >  1:26789675/1‑67 (MQ=255)

                          cTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATCGCTGGGGTACCTGGAt                                           >  1:34965512/1‑67 (MQ=255)

                          cTTGGCGATACCCCAATAACAGGGCGGTGAACCGCTTACACGAAGAAGATGGCTGGGGTACCTGGaa                                           >  1:10004038/1‑66 (MQ=39)

                           ttGGCGATACCGCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTc                                         >  1:7585742/1‑68 (MQ=255)

                           ttGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTc                                         >  1:32247675/1‑68 (MQ=255)

                           ttGGCGATACCCCAATAACCGGGCGGTGAAACGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTc                                         >  1:22184742/1‑68 (MQ=255)

                             ggCGATACCCCAATAACCGGGCTGTGCACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGa                                       <  1:41119612/68‑1 (MQ=255)

                             ggCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGa                                       <  1:30150557/68‑1 (MQ=255)

                              gCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGa                                       >  1:26047196/1‑67 (MQ=255)

                                gATACCCCAATAACCTGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAAc                                     <  1:37006069/67‑1 (MQ=255)

                                gATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAAcc                                    <  1:38233433/68‑1 (MQ=255)

                                gATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAAcc                                    >  1:20799189/1‑68 (MQ=255)

                                  tACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAg                                  <  1:34594727/68‑1 (MQ=255)

                                     cccAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGa                               >  1:42560251/1‑68 (MQ=255)

                                         aTAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATg                            >  1:10991901/1‑67 (MQ=255)

                                         aTAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATg                            >  1:30328174/1‑67 (MQ=255)

                                         aTAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATg                            >  1:23273450/1‑67 (MQ=255)

                                          tAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGcc                          >  1:33763797/1‑68 (MQ=255)

                                           aaCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAGTGCCg                         >  1:18970490/1‑68 (MQ=255)

                                           aaCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCg                         >  1:4045044/1‑68 (MQ=255)

                                           aaCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCg                         >  1:23045829/1‑68 (MQ=255)

                                           aaCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCg                         >  1:5872173/1‑68 (MQ=255)

                                           aaCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCg                         >  1:16243231/1‑68 (MQ=255)

                                           aaCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCg                         >  1:7116841/1‑68 (MQ=255)

                                           aaCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCg                         >  1:29119604/1‑68 (MQ=255)

                                           aaCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCg                         >  1:27238117/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:19469229/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:5045897/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:43079503/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:37572409/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:5519112/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:748281/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:19987121/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:32878413/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:25583210/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:29465539/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:21735981/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:24797254/1‑68 (MQ=255)

                                            aCCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                        >  1:2274653/1‑68 (MQ=255)

                                             ccGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGt                       >  1:6235219/1‑68 (MQ=255)

                                              cGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTa                      >  1:16489253/1‑68 (MQ=255)

                                              cGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTa                      >  1:1428722/1‑68 (MQ=255)

                                                ggCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATc                    >  1:1403572/1‑68 (MQ=255)

                                                ggCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATc                    >  1:16399118/1‑68 (MQ=255)

                                                ggCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATc                    >  1:6987828/1‑68 (MQ=255)

                                                 gCGGTGATCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCa                   >  1:32677118/1‑68 (MQ=255)

                                                 gCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCa                   >  1:27990243/1‑68 (MQ=255)

                                                   ggTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCaaa                 >  1:39415945/1‑68 (MQ=255)

                                                       aaCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAAcc             >  1:7936896/1‑68 (MQ=255)

                                                       aaCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAAcc             >  1:1505685/1‑68 (MQ=255)

                                                        aCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCg            >  1:40915951/1‑68 (MQ=255)

                                                          cGCTTACTCGAATAAGATGGCTGGGGTACCTGGATTCGAAGCAGGGAATGCCGGTATCAAAAACCGGt          <  1:42360504/68‑1 (MQ=17)

                                                           gCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt          >  1:12328651/1‑67 (MQ=255)

                                                             ttACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTg         >  1:36396788/1‑66 (MQ=255)

                                                                cTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCtt     >  1:33324596/1‑67 (MQ=255)

                                                                cTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCtt     >  1:28513168/1‑67 (MQ=255)

                                                                cTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCtt     >  1:12926433/1‑67 (MQ=255)

                                                                 tCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTa    >  1:17223070/1‑67 (MQ=255)

                                                                  cGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTAcc  >  1:4166800/1‑68 (MQ=255)

CGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACC  >  CP009273/692246‑692379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`