New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 3256491 =862 (1.230)39 (0.070) 13/90 NT 5.7% coding (529/1209 nt) tdcD propionate kinase/acetate kinase C, anaerobic
?CP009273 3256545 = 589 (1.050)coding (475/1209 nt) tdcD propionate kinase/acetate kinase C, anaerobic

Only 100 of 1087 total aligned reads displayed.

TAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGT  >  CP009273/3256478‑3256611
                                                                   |                                                                  
 aGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAgg                                                                   <  1:11945390/68‑1 (MQ=255)
 aGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAg                                                                    >  1:4420808/1‑67 (MQ=255)
 aGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAg                                                                    >  1:24749724/1‑67 (MQ=255)
   ttatacggccTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGcc                                                                 <  1:19993400‑M1/58‑1 (MQ=255)
   cGGTGCGACGTGCCGTGGAAACCATAACGGCGGACACCTAACTCTTCATAATATTTCCACGGCAGGcc                                                                 <  1:30611409/68‑1 (MQ=255)
    ggTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGcc                                                                 <  1:43051582/67‑1 (MQ=255)
    ggTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGcc                                                                 <  1:54414993/67‑1 (MQ=255)
    ggTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCg                                                                >  1:205526/1‑68 (MQ=255)
    ggTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCg                                                                >  1:56728712/1‑68 (MQ=255)
    ggTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCg                                                                >  1:35875805/1‑68 (MQ=255)
    ggTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCg                                                                >  1:12226620/1‑68 (MQ=255)
     gTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGt                                                               >  1:24384212/1‑68 (MQ=255)
     gTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGt                                                               >  1:39205867/1‑68 (MQ=255)
     atacggcctgccgtggaaatattatgaagagttaggtgtacgccgttatggtttccacggcaGGCCGt                                                               <  1:28985871‑M2/6‑1 (MQ=255)
     atacggcctgccgtggaaatattatgaagagttaggtgtacgccgttatggtttccacggcaGGCCGt                                                               <  1:36511762‑M2/6‑1 (MQ=255)
     atacggcctgccgtggaaatattatgaagagttaggtgtacgccgttatggtttccacggcaGGCCGt                                                               <  1:27523786‑M2/6‑1 (MQ=255)
     atacggcctgccgtggaaatattatgaagagttaggtgtacgccgttatggtttccacggcaGGCCGt                                                               <  1:46888726‑M2/6‑1 (MQ=255)
      tGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGt                                                               <  1:32749076/67‑1 (MQ=255)
      tGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGt                                                               <  1:28473509/67‑1 (MQ=255)
      tGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGt                                                               <  1:27092090/67‑1 (MQ=255)
      tGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGt                                                               <  1:25996279/67‑1 (MQ=255)
      tGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGt                                                               <  1:23164323/67‑1 (MQ=255)
      tGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGt                                                               <  1:26538552/67‑1 (MQ=255)
      tGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGt                                                               <  1:8866156/67‑1 (MQ=255)
      tGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGt                                                               <  1:20266036/67‑1 (MQ=255)
      tGCGACGGGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGt                                                               <  1:45538411/67‑1 (MQ=255)
      ggCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGt                                                               <  1:17874449/66‑1 (MQ=255)
      ggCGACGGGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGt                                                               <  1:3507648/66‑1 (MQ=255)
       acggccTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGtat                                                             <  1:29652783‑M1/62‑1 (MQ=255)
         gACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTataa                                                           >  1:7096715/1‑68 (MQ=255)
            gTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTataaata                                                        >  1:58005019/1‑68 (MQ=255)
            gTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTataaata                                                        >  1:44998711/1‑68 (MQ=255)
              GCCGTGGAAACCATAACGGCGTACACGTAACTCTTAAAAATATTTCCACCGCAGTCAGTATAAATAAg                                                      <  1:7543864‑M1/68‑1 (MQ=255)
              GCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAg                                                      >  1:55501192‑M1/1‑68 (MQ=255)
              GCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAg                                                      >  1:38253719‑M1/1‑68 (MQ=255)
                CGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCt                                                    >  1:16456530‑M1/1‑68 (MQ=255)
                CGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCt                                                    >  1:49633131‑M1/1‑68 (MQ=255)
                   GGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTc                                                  >  1:3125229‑M1/1‑67 (MQ=255)
                        ccATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAg                                             >  1:14218002/1‑67 (MQ=255)
                        ccATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGc                                            <  1:1567273/68‑1 (MQ=255)
                           tAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCAt                                         <  1:53432664/68‑1 (MQ=255)
                             aCGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCg                                       >  1:21359966/1‑68 (MQ=255)
                             aCGGCGTACACCTAACTCTTCATAATAATTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCg                                       >  1:13415827/1‑68 (MQ=255)
                               ggCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCg                                       >  1:47294037/1‑66 (MQ=255)
                               ggCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCg                                       >  1:44565466/1‑66 (MQ=255)
                               ggCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCg                                       >  1:51723187/1‑66 (MQ=255)
                               ggCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCg                                       >  1:22655688/1‑66 (MQ=255)
                               ggCGTACACCTAACTCTTCATAATATTTCCACAGCAGGCCGTATAAATAAGCTTCCGGAGCCATCg                                       >  1:3461854/1‑66 (MQ=255)
                                  gTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCtgg                                  <  1:38684112/68‑1 (MQ=255)
                                    acacCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCtggt                                 <  1:40380237/67‑1 (MQ=255)
                                       ccTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGaa                             >  1:33258320/1‑68 (MQ=255)
                                       ccTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGaa                             >  1:53890685/1‑68 (MQ=255)
                                       ccTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGaa                             >  1:17003274/1‑68 (MQ=255)
                                       ccTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGaa                             >  1:56069773/1‑68 (MQ=255)
                                       ccTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGaa                             >  1:41106856/1‑68 (MQ=255)
                                       ccTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGaa                             >  1:18790493/1‑68 (MQ=255)
                                         tAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGaa                             >  1:9524360/1‑66 (MQ=255)
                                          aaCTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACt                          <  1:43617809/68‑1 (MQ=255)
                                          aaCTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACt                          <  1:42031230/68‑1 (MQ=255)
                                           aCTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTg                         >  1:23935693/1‑68 (MQ=255)
                                           aCTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTg                         <  1:30171951/68‑1 (MQ=255)
                                           aCTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTg                         >  1:4772833/1‑68 (MQ=255)
                                            ctctTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTgg                        >  1:11436794/1‑68 (MQ=255)
                                            ctctTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTgg                        >  1:17406311/1‑68 (MQ=255)
                                            ctctTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTgg                        >  1:22094216/1‑68 (MQ=255)
                                            ctctTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTgg                        >  1:57421361/1‑68 (MQ=255)
                                             tctTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGt                       <  1:39980685/68‑1 (MQ=255)
                                             tctTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGt                       >  1:18280059/1‑68 (MQ=255)
                                             tctTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGt                       <  1:55034782/68‑1 (MQ=255)
                                              ctTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTa                      >  1:3425098/1‑68 (MQ=255)
                                              ctTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTa                      >  1:10993351/1‑68 (MQ=255)
                                              ctTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTa                      >  1:37512228/1‑68 (MQ=255)
                                              ctTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTa                      >  1:14699730/1‑68 (MQ=255)
                                               ttCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTAt                     <  1:28071805/68‑1 (MQ=255)
                                               ttCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTAt                     <  1:31697196/68‑1 (MQ=255)
                                               ttCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTAt                     <  1:32014692/68‑1 (MQ=255)
                                                 cATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCa                   <  1:20956424/68‑1 (MQ=255)
                                                  ataataTTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCaa                  >  1:13848343/1‑68 (MQ=255)
                                                   taataTTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCaaa                 >  1:46024949/1‑68 (MQ=255)
                                                    aataTTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAAt                <  1:7997195/68‑1 (MQ=255)
                                                     atatTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAAGTGGTATCAAAt                >  1:19454612/1‑67 (MQ=255)
                                                     atatTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAAt                >  1:41630594/1‑67 (MQ=255)
                                                     atatTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAAt                >  1:2537984/1‑67 (MQ=255)
                                                     atatTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAAt                >  1:52282123/1‑67 (MQ=255)
                                                     atatTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAAt                >  1:42404872/1‑67 (MQ=255)
                                                     atatTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAAt                >  1:33952041/1‑67 (MQ=255)
                                                     atatTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAAt                >  1:48929954/1‑67 (MQ=255)
                                                     atatTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAAt                >  1:36290420/1‑67 (MQ=255)
                                                       gtttccacggcaGGCCGTATAAATAAGCTTCCGGGGCCATCGTCTGGTGGAAACTGGTAGCAAATAcc             >  1:25652988‑M2/13‑68 (MQ=255)
                                                       atTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATAcc             >  1:10116112/1‑68 (MQ=255)
                                                        tttccagggcaGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCg            <  1:12569331‑M2/57‑1 (MQ=255)
                                                          tccacggcaGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGcc          >  1:52841850‑M2/10‑68 (MQ=255)
                                                          tccacggcaGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGcc          >  1:16149941‑M2/10‑68 (MQ=255)
                                                          tccacggcaGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGcc          >  1:4830930‑M2/10‑68 (MQ=255)
                                                          tccacggcaGGCCGTATAAATAAGCTTCCGGAGCCAGCGTCTGGTGGAAACTGGTATCAAATACCGcc          >  1:50968068‑M2/10‑68 (MQ=255)
                                                           ccacggcaGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCa         <  1:50235465‑M2/60‑1 (MQ=255)
                                                             acggcaGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCAcc       <  1:10525918‑M2/62‑1 (MQ=255)
                                                             acggaaGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTTGTGGAAACTGGTATCAAATACCGCCAcc       <  1:6404957‑M2/62‑1 (MQ=255)
                                                                gcaGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGa    >  1:3716775‑M2/4‑68 (MQ=255)
                                                                  aGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAg   <  1:15118618‑M2/66‑1 (MQ=255)
                                                                   |                                                                  
TAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGT  >  CP009273/3256478‑3256611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.