| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 161,088 | T→G | 17.9% | intergenic (+67/‑129) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 161,088 | 0 | T | G | 17.9% | 1410.2 / 63.9 | 560 | intergenic (+67/‑129) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base T (35/374); new base G (80/2); total (183/377) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 807 total aligned reads displayed.
GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTC > CP009273/161022‑161155 | ttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctca < 1:16305182/67‑1 (MQ=255) ttggttTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctca < 1:32456521/67‑1 (MQ=255) ttggttTTCCCCCTCCCCCTGGGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctca < 1:19960506/67‑1 (MQ=18) tgtttttCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc < 1:27083048/65‑1 (MQ=255) tgtttttCTCCCGCGCCGTGGGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc < 1:17533641/65‑1 (MQ=18) tggttTTCTCCCTCTCCCTGTGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc < 1:8665346/68‑1 (MQ=255) tggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc < 1:11210923/68‑1 (MQ=255) tggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc < 1:3615222/68‑1 (MQ=255) tggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc < 1:13067398/68‑1 (MQ=255) tggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc < 1:31642731/68‑1 (MQ=255) tggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc < 1:44910937/68‑1 (MQ=255) tggttTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc < 1:54025515/68‑1 (MQ=255) tggttTTCTCCCTCTCCCGGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc < 1:10019478/68‑1 (MQ=255) tggttTTCACCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc < 1:49577446/68‑1 (MQ=255) ggtttttCCCCCTCCCCTGGGGGGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTtcaccctcacc < 1:33560183/65‑1 (MQ=16) ggttTTCGCCCACCCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc < 1:13981906/67‑1 (MQ=18) ggttTGCCCCCTGGCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc < 1:26769114/67‑1 (MQ=18) tttCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTaa < 1:43941153/68‑1 (MQ=255) ttCTCCCTCCCCGTGGGGTAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAc < 1:40989725/68‑1 (MQ=21) ttCTCCCTCCCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAc < 1:19407217/68‑1 (MQ=255) tctccctctcccTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 1:28776606/68‑1 (MQ=255) ccccctccccgtGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAccc < 1:55479886/66‑1 (MQ=21) tccctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCGCACCCTAAacc > 1:57199959/1‑67 (MQ=17) accctctccctGTGGGTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCTCCCTCACCCTAACCct < 1:34131737/67‑1 (MQ=21) ccctctccctGTGGGAGAGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc < 1:41742147/68‑1 (MQ=255) ctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc < 1:27658041/68‑1 (MQ=255) ctctccctGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc < 1:40398047/68‑1 (MQ=255) ctcccccgGGGGGAGAGGGCCGGGGGGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc < 1:13715738/68‑1 (MQ=25) tctccctGGGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 1:44688655/68‑1 (MQ=25) tccccctGCGGGCGCGGGCCGGGCGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 1:50848033/65‑1 (MQ=25) ccgccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 1:32956349/65‑1 (MQ=255) ctccctGTGGGATAGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTccc < 1:3942063/68‑1 (MQ=25) ctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTccc < 1:25752778/68‑1 (MQ=255) ctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTccc < 1:9188567/68‑1 (MQ=255) ctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTccc < 1:27970052/68‑1 (MQ=255) ctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTccc < 1:22003097/68‑1 (MQ=255) ctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTccc < 1:34972372/68‑1 (MQ=255) ctccctGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTccc < 1:57662142/68‑1 (MQ=255) tccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCGCCCCCGAACCCTCTccc > 1:51390398/1‑67 (MQ=18) tccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTccc < 1:14327416/67‑1 (MQ=255) tccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCAACCCCACCCGAACCCTCGccc > 1:39636327/1‑67 (MQ=17) cccctGTGGGCTTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTccc < 1:45392980/66‑1 (MQ=37) ccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTc < 1:23974117/68‑1 (MQ=255) ccctGGGGGTGTGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTc < 1:5194452/68‑1 (MQ=37) ccctGGGGGTGCGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTc < 1:31066132/68‑1 (MQ=37) ccctGGGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTc < 1:7527665/68‑1 (MQ=255) ccctGGGGGAGCGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTc < 1:49255451/68‑1 (MQ=37) ccctGGGGGAGAGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTc < 1:52667300/68‑1 (MQ=255) cctGTGGGTTAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa < 1:22997381/68‑1 (MQ=255) cctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCGCACCCAAACCCCCGcccgcc > 1:47006186/1‑65 (MQ=17) cctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTc < 1:30457075/67‑1 (MQ=255) cctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa < 1:54461886/68‑1 (MQ=255) tgtgGGAGCGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 1:18057716/68‑1 (MQ=255) tgtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 1:38114346/68‑1 (MQ=255) tgtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCAAACCCACTCCCGCAAg > 1:42998927/1‑68 (MQ=21) tgggggAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 1:40577203/65‑1 (MQ=255) gggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAggg < 1:12067833/67‑1 (MQ=255) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGCCCCCCCCCCCAAACCACACCCCCAAggggg > 1:17029432/1‑66 (MQ=9) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGCCCCCCACCCCAACCCCCACCCCCAAcgggg > 1:50075674/1‑66 (MQ=12) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCACACCCAAAACCACACCCCCAAagggg > 1:48400868/1‑66 (MQ=12) gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag < 1:10829547/67‑1 (MQ=255) gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag < 1:36788158/67‑1 (MQ=255) gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCGAACCCTCACCCGCAAgggggg > 1:37430021/1‑65 (MQ=21) agagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCAGACCCAAAACCACTCCACCAAGGgatgg > 1:4777478/1‑65 (MQ=18) agagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACCCCCGAACCCGCTCCCACAATGgtgac > 1:22465269/1‑67 (MQ=18) agagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACCCCCAAACCCACACCCGCAACGgagcg > 1:7032333/1‑66 (MQ=18) agGGCCGGGGTGAGGGCATCAGCGCGCACGTTGCCCCCCACCCAGACCCACTCCCGGCAGGGAGCggg > 1:42488505/1‑68 (MQ=12) agGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCCCCCAAACCCTGTCCCGGAAGGGGGCggg > 1:30054769/1‑68 (MQ=18) agGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAAACCAATACCCAAAGGGAGAggg > 1:52335157/1‑68 (MQ=18) gggCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCCCACCCTAACCCTCTCCCTCAAGGGAGAggg < 1:56365414/67‑1 (MQ=38) gggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 1:56788521/67‑1 (MQ=255) gcgggggggAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgggg < 1:43297937/63‑1 (MQ=25) gCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 1:41537988/67‑1 (MQ=255) gCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 1:47507478/67‑1 (MQ=255) gCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 1:15589007/67‑1 (MQ=255) gCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 1:6613756/67‑1 (MQ=255) ggTGAGGGCATCAGCGCGCACGTTGACCCACACCCAAAACCACACCCCCAAAGGGGAGGGGGCCGCTc > 1:29292751/1‑68 (MQ=12) ggTGAGGGCATCAGCGCGCACGTTCACCCTCCCCCTAACCCTCTCCCGCAAGGGAGCGGGGGCCGGTc > 1:26200270/1‑68 (MQ=37) ggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAgg > 1:25509557/1‑53 (MQ=255) ggTGAGGGCATCAGCGCGCACGTTCACCCACCCCCGAACCCCCTACCACAAGGGGGGGGGGGCCgctg > 1:35148657/1‑65 (MQ=16) ggccATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca < 1:20539197/65‑1 (MQ=255) ggCATCAGCGCGCACGTTCACCCGCACCCTAACCCACTCCCTCAAGGGAGAGGGGGCCGCTCGAGccc > 1:29785433/1‑66 (MQ=25) gCATCAGCGCGCACGTTCACCCTCACCCTAACCCACTCCCTCAAGGGAGAGGGGCCCGATCGAGcaca > 1:11665968/1‑68 (MQ=255) gCATCAGCGCGCACGTTCACCCGCCCCCGAACCCTCTACCGCAAGGGAGAGGGGGCCGCTAGCGcaca > 1:23340641/1‑68 (MQ=255) aTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACaaa < 1:40027805/68‑1 (MQ=255) aTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACaaa < 1:53402233/68‑1 (MQ=255) cAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtt < 1:14813030/68‑1 (MQ=255) aGCGCGCACGTTCACCCTCACCCAAACCCACACCCACAACGGGGAGGGGCCCGCTCGAGCACAAAatt > 1:38913739/1‑68 (MQ=255) aCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagaga < 1:54858341/68‑1 (MQ=255) aCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagaga < 1:18716367/68‑1 (MQ=255) cGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGat < 1:21550493/68‑1 (MQ=255) tcaccctcacccTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat < 1:24673378/67‑1 (MQ=255) tcaccctcacccTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat < 1:25173173/67‑1 (MQ=255) tcaccctcacccTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat < 1:46080425/67‑1 (MQ=255) tcaccctcacccTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat < 1:3771598/67‑1 (MQ=255) caccctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtct < 1:15142206/68‑1 (MQ=255) accctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtctt < 1:28419539/68‑1 (MQ=255) ccctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttc < 1:12498250/68‑1 (MQ=255) cctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttct < 1:38479746/68‑1 (MQ=255) actcaccatAACCCTCTCCCGCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttct < 1:20992604/67‑1 (MQ=38) | GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTC > CP009273/161022‑161155 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |