| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,222,671 | G→C | 10.4% | intergenic (+127/+246) | yohP → / ← dusC | uncharacterized protein/tRNA‑dihydrouridine synthase C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,222,671 | 0 | G | C | 10.4% | 260.2 / 6.0 | 111 | intergenic (+127/+246) | yohP/dusC | uncharacterized protein/tRNA‑dihydrouridine synthase C |
| Reads supporting (aligned to +/- strand): ref base G (9/84); new base C (8/2); total (25/86) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.86e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 155 total aligned reads displayed.
GCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATC > CP009273/2222606‑2222738 | gcTCCTGTCATGTGCATTTCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATcgcc < 1:44997805/68‑1 (MQ=255)gcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATcgcc < 1:43347757/68‑1 (MQ=255)gcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATcgcc < 1:23909641/68‑1 (MQ=255)gcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATcgcc < 1:48022774/68‑1 (MQ=255) tCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCG‑G‑‑CGGGGGGG‑GG‑CCAA‑cgccg > 1:6282074/1‑67 (MQ=25) ccTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATcgccgcc < 1:51653182/68‑1 (MQ=255) tGTCCTGTGCCTTGCTTCATATACTCTCTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATcgccgccgc < 1:14931022/68‑1 (MQ=18) tGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATcgccgccgc < 1:38191745/68‑1 (MQ=255) tGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATcgccgccgc < 1:48994421/68‑1 (MQ=255) tGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATcgccgccgc < 1:53753435/68‑1 (MQ=255) gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCgccgccgcc < 1:23930083/68‑1 (MQ=255) gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCgccgccgcc < 1:4916283/68‑1 (MQ=255) cATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGcccc < 1:57461167/68‑1 (MQ=255) cATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGcccc < 1:3365499/68‑1 (MQ=255) cATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGccc < 1:24676092/67‑1 (MQ=255) cATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGccc < 1:56821442/67‑1 (MQ=255) cATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGccc < 1:10469799/67‑1 (MQ=255) cATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGccc < 1:40224675/67‑1 (MQ=255) cATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGccc < 1:49100376/67‑1 (MQ=255) cATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGccc < 1:43111201/67‑1 (MQ=255) cATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGccc < 1:14141919/67‑1 (MQ=255) cATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGccc < 1:13662489/67‑1 (MQ=255) cATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGccc < 1:6460446/67‑1 (MQ=255) cATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGccc < 1:8639963/67‑1 (MQ=255) cATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGccc < 1:1246604/67‑1 (MQ=255) cATGTGCATTGATTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGccc < 1:56186392/67‑1 (MQ=255) tgtgCATTGCTTCATATAATCACTGGCGCAAGGCGCGCG‑G‑‑GCGGGGGC‑GG‑CCCAAcccccccccccc > 1:47492133/1‑67 (MQ=255) tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCG‑G‑‑CGGGGGGG‑GG‑CCCAAcccccccccccc > 1:28407694/1‑67 (MQ=19) tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCG‑G‑‑CGGGGGGG‑GG‑CCAACcccccccccccc > 1:9127732/1‑67 (MQ=21) tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑GGGGGGGCGGG‑CCAAAcccccccccccc > 1:49918469/1‑67 (MQ=21) tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑GCCGGGGG‑GG‑CCCAAAGCccccccccccc > 1:20943921/1‑67 (MQ=19) tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑GCCGGGGC‑GG‑GCCAAAGCccccccccccc > 1:14506666/1‑67 (MQ=19) tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCt < 1:46204961/68‑1 (MQ=255) tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCt < 1:17814696/68‑1 (MQ=255) tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCt < 1:12730945/68‑1 (MQ=255) tgtgCATTGCTGCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCt < 1:20716281/68‑1 (MQ=255) gCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGG‑‑CGGGGGGG‑GG‑CCAAccccccccccccccc > 1:18633335/1‑66 (MQ=16) gCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑GCCCGGGGGGC‑GC‑CCAAccccccccccccccc > 1:29936407/1‑66 (MQ=16) gCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑GC‑CGGGGGGG‑GG‑CCAAACcccccccccccccc > 1:41995420/1‑65 (MQ=16) gCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑GAGGGGGG‑GG‑GCCAAAGCccccccccccacc > 1:26230534/1‑64 (MQ=16) gCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGG‑GG‑CCAATCgcccccgcccccacc > 1:28678889/1‑64 (MQ=18) tttGCTTCAAATAATCACTGGCCCAAGCAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCTG‑CAcc < 1:35007641/67‑1 (MQ=17) aTTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑GGGGGGGG‑GG‑GCCCACGCCCCCGGCCCCCG‑ccc > 1:5290171/1‑65 (MQ=16) aTTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGG‑GC‑CCAAAC‑CCCccgccccccg‑cccc > 1:27631117/1‑67 (MQ=18) aTTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCACTCGCCGCCGCCCCCTG‑CAcc < 1:54058249/68‑1 (MQ=255) aTTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCTG‑CAcc < 1:51798147/68‑1 (MQ=255) aTTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCTG‑CAcc < 1:36743664/68‑1 (MQ=255) aTTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCTG‑CAcc < 1:42743237/68‑1 (MQ=255) aTTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCTG‑CAcc < 1:17909468/68‑1 (MQ=255) tGCTTCATATAATCCCTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCTG‑CAcccc < 1:40769736/68‑1 (MQ=255) tGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCTG‑CAcccc < 1:15996950/68‑1 (MQ=255) tGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCGG‑CAcccc < 1:31031612/68‑1 (MQ=255) tGCTTAATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CTGGGGGC‑GG‑CAAATCGCCGCCGCCCCCTG‑CAcccc < 1:55734704/68‑1 (MQ=17) tGCTTAATAAAATCACTGGCCCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAAGCGCCGCCGCCCCCTG‑CAcccc < 1:18167135/68‑1 (MQ=14) gCTTCATATAATCACTGGCGCAAGGAGCGCG‑G‑‑CGGGGGGG‑GG‑CCAA‑AACCGCCCCCCCCCG‑cccccc > 1:57218695/1‑67 (MQ=12) gCTTCATATAATCACTGGCGCAAGGAGCGC‑‑T‑‑CAGGGGGG‑GG‑CCCATCGACCCCCCCCCCAG‑GAcccc > 1:7128551/1‑67 (MQ=17) gCTTCATATAATCACTGGCGCAAGGAGCGC‑‑T‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCTG‑CAccccc > 1:38410502/1‑68 (MQ=255) gCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑GCGGGGGG‑GG‑CCCAAAACCCCCCCCCCCCG‑cccccc > 1:25652955/1‑67 (MQ=12) ttCATATAATCACTGGCGCACGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAAGCGCCGCCGCCCCCTG‑CACCCCCgg < 1:18945715/68‑1 (MQ=17) ttCATATAATCACTGGCGCAAGGAGCGCG‑G‑‑GGGGGGGG‑GG‑CCAAAC‑CCCCCCCCCCCCC‑CAccccccc > 1:1354996/1‑66 (MQ=12) ttCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCTG‑CACCCCCgg < 1:32571285/68‑1 (MQ=255) cATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCTG‑CACCCCCGGGc < 1:32102786/68‑1 (MQ=255) ataATCACTGGCGCAAGGAGCGCGGG‑‑GGGGGGGG‑GG‑CCAACCCCCCCCCCCCCCCC‑CCCCCCCggggg > 1:56472388/1‑66 (MQ=9) ataATCACTGGCGCAAGGAGCGCGGG‑‑GGGGGGGG‑GG‑CCAACCCCCCCCCCCCCCCC‑CCCCCCCGGGcg > 1:16675692/1‑67 (MQ=9) ataATCACTGGCGCAAGGAGCGCG‑G‑‑GGGGGGGG‑GG‑CCAAAGCCCCCCCCCCCCCC‑CCCCCCCggggcg > 1:39260314/1‑65 (MQ=9) ataATCACTGGCGCAAGGAGCGCG‑G‑‑CGGGGGGG‑GGCCCAAGCCCCCCCCCCCCCCC‑CCCCCCCggccg > 1:37465600/1‑65 (MQ=9) ataATCACTGGCGCAAGGAGCGCG‑G‑‑CGGGGGGG‑GG‑CCAAAC‑CCCCCCCCCCCCC‑CACCCCCCGGcgca > 1:13044263/1‑65 (MQ=9) ataATCACTGGCGCAAGGAGCGCG‑G‑‑CGGGGGGG‑GG‑CCAAAC‑CCCCCCCCCCCAG‑CCCCCCCCGGcgcc > 1:9681212/1‑65 (MQ=9) ataATCACTGGCGCAAGGAGCGC‑‑G‑‑GCGGGGGG‑GG‑GCCCGCGCCGCCCCCCCCCGCCGCCCCCgggggc > 1:46986302/1‑65 (MQ=9) ataATCACTGGCGCAAGGAGCGC‑‑G‑‑GCGGGGGG‑GG‑GCCAGAACCCCCCCCCCCCG‑CCCCCCCgcgcggg > 1:32123218/1‑65 (MQ=9) aTCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCTG‑CACCTCCGGGCTCTGGc > 1:19991321/1‑68 (MQ=18) ccAAACCCCTCCGCCCCCGG‑CACCCCCGGGCTATGGCGAACAACATCGCCGCTTCGCGGTGCCCTCgg < 1:13142580/68‑1 (MQ=11) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg < 1:416818/67‑1 (MQ=31) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg < 1:9440284/67‑1 (MQ=31) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg < 1:36640120/67‑1 (MQ=31) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg < 1:6155064/67‑1 (MQ=31) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg < 1:35502671/67‑1 (MQ=31) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg < 1:33130932/67‑1 (MQ=31) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg < 1:53752248/67‑1 (MQ=31) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg < 1:24493621/67‑1 (MQ=31) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg < 1:2724906/67‑1 (MQ=31) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg < 1:8032660/67‑1 (MQ=31) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg < 1:29460679/67‑1 (MQ=31) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg < 1:27580573/67‑1 (MQ=31) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc < 1:1241044/68‑1 (MQ=33) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc < 1:44800177/68‑1 (MQ=33) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc < 1:45396358/68‑1 (MQ=33) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc < 1:30395434/68‑1 (MQ=33) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc < 1:44534212/68‑1 (MQ=33) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc < 1:12876634/68‑1 (MQ=33) cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc < 1:10273968/68‑1 (MQ=33) cAATCGCCGCCGCCCCCGG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg < 1:50818807/67‑1 (MQ=14) cAATCGCCGCCGCCCCCGG‑CAACCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc < 1:54421804/68‑1 (MQ=255) cAATCCCGGCCGCCCCCGG‑CACCCACTGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc < 1:27388975/68‑1 (MQ=11) cgccgccgcCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAt < 1:19637571/68‑1 (MQ=35) cgccgccgcCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAt < 1:740269/68‑1 (MQ=35) cgccgccgcCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAt < 1:40994360/68‑1 (MQ=35) gccgccgccCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAt > 1:32516254/1‑67 (MQ=35) gccgccgccCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAt > 1:45078250/1‑67 (MQ=35) gccgccgccCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAt > 1:45656766/1‑67 (MQ=35) | GCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑‑G‑‑CAGGGGGC‑GG‑CCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATC > CP009273/2222606‑2222738 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |