| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,711,673 | A→C | 7.5% | V72G (GTT→GGT) | yfiF ← | putative methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,711,673 | 0 | A | C | 7.5% | 1317.2 / 14.8 | 468 | V72G (GTT→GGT) | yfiF | putative methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (328/99); new base C (3/38); total (331/137) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.24e-19 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 481 total aligned reads displayed.
AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGG > CP009273/2711607‑2711740 | aaaCTTTAAGCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAAc < 1:23041486/68‑1 (MQ=255) aCTTTTACGACTGATGCCACCGTGATCGGCCATTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑g > 1:6980043/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑g > 1:17085682/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑g > 1:48706969/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑g > 1:6138797/1‑68 (MQ=255) cTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑Gt > 1:24078982/1‑68 (MQ=255) ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑Gt > 1:29664872/1‑67 (MQ=255) ccACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGcc > 1:17865530/1‑67 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCAc > 1:13107379/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCAc > 1:37322867/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCAc > 1:53423780/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCAc > 1:28981939/1‑68 (MQ=255) aCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACg > 1:10118204/1‑68 (MQ=255) aCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACg > 1:41748714/1‑68 (MQ=255) tGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGc > 1:5033863/1‑68 (MQ=255) tGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGc > 1:47008219/1‑68 (MQ=255) tGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCgct > 1:8495850/1‑66 (MQ=255) tGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGaa > 1:38146131/1‑68 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGaa > 1:10692617/1‑66 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGaa > 1:15033757/1‑66 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGaa > 1:20389673/1‑66 (MQ=255) ccccGTGATCGGCCTTTTCCGGCGTCTCATCCCCCGGCGCGG‑GGTAAACC‑GGGCGCCACGGCGAATCt < 1:19742813/66‑1 (MQ=255) aCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCt > 1:50571410/1‑67 (MQ=255) ccGTTATCGGCATTTTCCGGGGTCTAACCCCCCGGCGCGC‑GGAAAACG‑GGGCGCCACGGCGAATCTTc < 1:7862001/68‑1 (MQ=255) ccGTGCTCGGCCTTTGCGGGGGTCTCATCCCCCGGCGCGC‑GGTAAACC‑GGGCGCCACGGCGAATCTTc < 1:1424913/68‑1 (MQ=255) ccGTGCGCGGCCTTTGCCGGCGTCCCATCACCCGGCGCGG‑GGGAACCC‑GGGCCCCACGGCGAATCTTc < 1:19418142/68‑1 (MQ=255) ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCtt > 1:47515350/1‑67 (MQ=255) ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCtt > 1:51662369/1‑67 (MQ=255) ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCtt > 1:54828146/1‑67 (MQ=255) ccGTGATCGCCCTTTCCCGGCGGCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTc < 1:9623946/68‑1 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCCCGGCGAATCTTc > 1:13455528/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTc > 1:24642666/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTc > 1:43544571/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTc > 1:32939792/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTc > 1:1638286/1‑67 (MQ=255) gTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTccc > 1:15421658/1‑68 (MQ=255) gATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTccc > 1:22006564/1‑66 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCCCtt > 1:51409428/1‑68 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACt < 1:26223586/67‑1 (MQ=255) cGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACtt > 1:7317972/1‑67 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCg > 1:56884070/1‑68 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCg > 1:45112777/1‑68 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCg > 1:33175891/1‑68 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCg > 1:21059554/1‑68 (MQ=255) ccTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCgg > 1:52156795/1‑68 (MQ=255) ccTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCgg > 1:14427569/1‑68 (MQ=255) ccTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCgg > 1:45939030/1‑68 (MQ=255) ccTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCgg > 1:11359483/1‑68 (MQ=255) ttttCGGGCGTCTCAACCCCCGGCGCGC‑GGGAAACG‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTc < 1:23298891/68‑1 (MQ=255) ccGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:14069599/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:42963970/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:44673203/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:3943374/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:33447184/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:28668198/1‑68 (MQ=255) ccGGCGGCTCACCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg < 1:11996567/68‑1 (MQ=255) cggcggCTCATCACCCGGCGCGC‑GGTAAACG‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg < 1:11063333/68‑1 (MQ=255) cGGCGTCTAACCCCCCGGCGCGC‑GGTAAACC‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg < 1:49776919/68‑1 (MQ=255) ggggTCTCATCCCCCGGCGCGG‑GGTAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:54115535/65‑1 (MQ=255) ggggTCTCAACCCCCGGCGCGG‑GGTAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:34381512/65‑1 (MQ=255) ggCGTCTCATCACCCGGCGCGG‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:25298580/68‑1 (MQ=255) ggCGTCTCACCCCCCGGCGCGC‑GGGAAACG‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:40136517/68‑1 (MQ=255) gCGGCTCACCCCCGGGCGCGG‑GGCAACCG‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:30074387/67‑1 (MQ=255) cGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg > 1:31230967/1‑65 (MQ=255) cGTCCCACCCCCCGGCGGGG‑GGTAACCG‑GGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGc < 1:27448223/67‑1 (MQ=255) gTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTg > 1:46446158/1‑68 (MQ=255) ctcATCCCCCGGCGCGG‑GGGAAACG‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa < 1:12469531/67‑1 (MQ=255) ctcATCCCCCGGCGCGC‑GGGAACCG‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa < 1:16195793/67‑1 (MQ=255) cacatcaCCCGGCGCGC‑GGGAAACC‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa < 1:39027275/65‑1 (MQ=255) aacatcaCCCGGCGCGG‑GTTAACCG‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc < 1:2142495/66‑1 (MQ=255) tcatccCCCGGCGCGG‑GGGAAACG‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAct < 1:36975184/68‑1 (MQ=255) tcatcaCCGGGCGGGG‑GGCAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAct < 1:23822207/68‑1 (MQ=38) cataCCCGGGGCGGGGGGCAACCGGTGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc < 1:20189390/64‑1 (MQ=255) cccccGGCGCGC‑GGCAACCG‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt < 1:12279283/66‑1 (MQ=255) caCCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt > 1:32699470/1‑68 (MQ=255) aCCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGCCTCTGtt > 1:4405982/1‑68 (MQ=255) aCCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt > 1:36164156/1‑68 (MQ=255) aCCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt > 1:46759220/1‑68 (MQ=255) aCCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt > 1:48124278/1‑68 (MQ=255) aCCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt > 1:27850088/1‑68 (MQ=255) cccGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCCCTTGCGGTCGCGGGGCTGACTCTGttt > 1:18068807/1‑68 (MQ=255) cccGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:45369523/1‑68 (MQ=255) cccGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:9149577/1‑68 (MQ=255) cccGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:35516195/1‑68 (MQ=255) cccGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:40807889/1‑68 (MQ=255) cccGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:26908948/1‑68 (MQ=255) cccGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:410911/1‑68 (MQ=255) cccGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:30676331/1‑68 (MQ=255) ccGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:28233914/1‑68 (MQ=255) ccGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:55392127/1‑68 (MQ=255) ccGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:42534589/1‑68 (MQ=255) ccGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:24915028/1‑68 (MQ=255) cGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:18326611/1‑67 (MQ=255) cgcgc‑gGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCa > 1:37823259/1‑67 (MQ=255) gcgc‑gGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATc > 1:5252050/1‑68 (MQ=255) cgc‑gGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATc > 1:31591649/1‑67 (MQ=255) ggAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTGACGGGGCTGACTCTGTTTGTCATCgcgg > 1:57614681/1‑67 (MQ=255) taaaCC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcggg < 1:56319096/66‑1 (MQ=255) aCC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg < 1:32142237/68‑1 (MQ=255) aCC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg > 1:47834497/1‑68 (MQ=255) | AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGC‑GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGG > CP009273/2711607‑2711740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |