| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 161,106 | T→G | 9.6% | intergenic (+85/‑111) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 161,106 | 0 | T | G | 9.6% | 2746.3 / 38.7 | 888 | intergenic (+85/‑111) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base T (318/451); new base G (76/3); total (434/454) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-23 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 897 total aligned reads displayed.
TCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAA > CP009273/161039‑161173 | ccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTc < 1:23974117/68‑1 (MQ=255) cctGTGGGTTAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCa < 1:22997381/68‑1 (MQ=255) cctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTc < 1:30457075/67‑1 (MQ=255) cctGGGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCa < 1:45711963/68‑1 (MQ=255) ctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCaa < 1:50130280/68‑1 (MQ=255) ctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCACTCCCGCaa > 1:23383306/1‑68 (MQ=255) tgtgGGAGCGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAg < 1:18057716/68‑1 (MQ=255) tgtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑GACCCACCCCCAAACCCACACCCGCAAg > 1:28501217/1‑68 (MQ=17) tgtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAg < 1:56842644/68‑1 (MQ=255) tgtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAg < 1:55055499/68‑1 (MQ=255) tgggggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAg < 1:54272078/65‑1 (MQ=255) tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑GCCCCGCACCCAAACCCACAACCCCAAggg > 1:75104/1‑68 (MQ=17) tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑GACCCACACCCGAACCCTCGCCCGCAAggg > 1:52630681/1‑68 (MQ=21) tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CCCCCGCCCCCGAACCCTCTCCCTCAAggg > 1:52400452/1‑68 (MQ=21) tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CCCCCAAACCCAAACCCACTCCCACAAggg > 1:27921556/1‑68 (MQ=18) ggAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGga < 1:8706557/67‑1 (MQ=255) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑GACCCACACCCAAAACCACACCCCCAAagggg > 1:48400868/1‑66 (MQ=12) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCGCACCCTAAACCGCTCCCGCTAggggg > 1:34803466/1‑66 (MQ=18) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCCCACCCAAACCCACTCCCGCAAtggc > 1:33012007/1‑66 (MQ=21) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCACACCCTAACCCCCTACCCCAAGGgag > 1:12050096/1‑68 (MQ=25) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑AACCCACACCCTAAACCACGCCCCCAAggggg > 1:4285384/1‑66 (MQ=18) gagtGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGgag < 1:29465089/67‑1 (MQ=255) gagtGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGgag < 1:24720527/67‑1 (MQ=255) gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGgag < 1:12592434/67‑1 (MQ=255) gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGgag < 1:10894426/67‑1 (MQ=255) gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGgag < 1:36550956/67‑1 (MQ=255) gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGgag < 1:6077402/67‑1 (MQ=255) agagGGGCGGGGTGAGGGCATCTGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCGTCAAGGgaga < 1:32247147/67‑1 (MQ=37) agagGGCCGGGGTGAGGGCATCAGCGCGCACGTTGCACCCGCCCCAAAACCCCAGCCCGCAAgggggg > 1:6748913/1‑65 (MQ=9) agagGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCGCACCCGAACCCACTCCCGCAAGGGagag > 1:29979695/1‑68 (MQ=25) agGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑GACCCGCGCCCGAACCCGCGCCCGCAAGgggggggg > 1:35129810/1‑68 (MQ=12) agGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CCCCCGCACCCTAAACCAATCCCGCAAGGGAGAggg > 1:27600976/1‑68 (MQ=21) gggCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 1:13227387/67‑1 (MQ=255) gggCCGGGGGGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 1:37001212/67‑1 (MQ=255) gggCCGGGGGGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgg < 1:3868330/66‑1 (MQ=255) ggCGGGGGTGCGGCCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgggg < 1:33934627/67‑1 (MQ=37) gcgggggTGAGGGCATCAGCGCGCACGTT‑CACGCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 1:11344035/64‑1 (MQ=255) gCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 1:40438913/67‑1 (MQ=255) gCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 1:47507478/67‑1 (MQ=255) gCCGGGGGGCGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 1:43158843/67‑1 (MQ=255) ggTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAt < 1:43643603/67‑1 (MQ=255) ggTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAt < 1:49402408/67‑1 (MQ=255) gTGAGGGCATCAGCGCGCACGTT‑CCCCCGCACCCGAACCCACACCCCCCACGGAGAGGGGGCCGCTCg > 1:53103886/1‑68 (MQ=16) gTGAGGGCATCAGCGCGCACGTT‑CCCCCCCCCCCTAAACCACTCCCGCAAGGGGGCGGGGGCCGATc > 1:53561441/1‑67 (MQ=19) gTGAGGGCATCAGCGCGCACGTT‑CCCCCCCCCCCGAACCCGCTCCCACAACGGCGAGGGGGCCGCTc > 1:37936469/1‑67 (MQ=16) gcgggCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 1:17492242/66‑1 (MQ=255) gAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 1:16495240/68‑1 (MQ=255) gCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGTGAGGGGACCGCTCGAGcaca > 1:54622747/1‑68 (MQ=255) aTCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACaaa < 1:37539048/68‑1 (MQ=255) aTCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACaaa < 1:55784088/68‑1 (MQ=255) gcgcACGTT‑GACCCACACCCCAAACCACACCCGCAAGCGAGAGGGGACCGCGCGCGCACAAAatttta > 1:41325415/1‑66 (MQ=255) gcgcACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:33473859/68‑1 (MQ=255) gcACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGTCCGATCGAGCACAAATTTTgag < 1:42285499/67‑1 (MQ=255) gcACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga < 1:28977552/68‑1 (MQ=255) gcACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga < 1:48968108/68‑1 (MQ=255) aCGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagaga < 1:18716367/68‑1 (MQ=255) aCGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagaga < 1:14184884/68‑1 (MQ=255) t‑caccctcacccTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat < 1:4511925/67‑1 (MQ=255) caccctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtct < 1:9370588/68‑1 (MQ=255) accctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtctt < 1:10109390/68‑1 (MQ=255) accctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtctt < 1:15474004/68‑1 (MQ=255) ccctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttc < 1:40159165/68‑1 (MQ=255) cctcaccctAACCGTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttct < 1:52026986/68‑1 (MQ=255) cctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttct < 1:39844982/68‑1 (MQ=255) cctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttct < 1:47976479/68‑1 (MQ=255) cctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttct < 1:46571826/68‑1 (MQ=255) cctcaacctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttct < 1:56385519/68‑1 (MQ=255) tcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc < 1:21058679/68‑1 (MQ=255) ccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGt < 1:17210454/68‑1 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat < 1:38981234/57‑1 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttct > 1:719808/1‑62 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttct > 1:15158382/1‑62 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttct > 1:39378177/1‑62 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttct > 1:20225503/1‑62 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:31664028/68‑1 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:13823811/68‑1 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:25610565/68‑1 (MQ=255) aaCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 1:22425415/68‑1 (MQ=255) aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTga > 1:44613198/1‑67 (MQ=255) aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt > 1:10486859/1‑68 (MQ=255) aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt > 1:2161483/1‑68 (MQ=255) aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt > 1:14647758/1‑68 (MQ=255) aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt > 1:19247220/1‑68 (MQ=255) aCCCTCTCCCTCAAGGGAGA‑GGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa < 1:27156082/68‑1 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa > 1:57431022/1‑68 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:25203918/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:8052173/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:36110956/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:31235629/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:38333656/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:46954880/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:26535534/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:26087871/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:41816707/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:51633643/1‑67 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa > 1:50744448/1‑67 (MQ=255) tctcCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa < 1:20673081/68‑1 (MQ=255) tcCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga > 1:19942280/1‑68 (MQ=255) ccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaag > 1:15817505/1‑68 (MQ=255) ccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaag > 1:40857483/1‑68 (MQ=255) | TCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTT‑CACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAA > CP009273/161039‑161173 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |