| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,263,466 | G→T | 17.5% | intergenic (+505/+109) | yhaC → / ← rnpB | pentapetide repeats‑related protein/RNase P, M1 RNA component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,263,466 | 0 | G | T | 17.5% | 967.4 / 37.2 | 416 | intergenic (+505/+109) | yhaC/rnpB | pentapetide repeats‑related protein/RNase P, M1 RNA component |
| Reads supporting (aligned to +/- strand): ref base G (209/108); new base T (26/49); total (251/165) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.58e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 420 total aligned reads displayed.
AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCT > CP009273/3263399‑3263533 | aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTg > 1:43613024/1‑68 (MQ=35) gTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCGCAGCGCCTTTATACGTAAACCAGGTgt > 1:38310503/1‑67 (MQ=11) ttatcggtcagtttcacctgatttacgtaaaaacccgcttcgGCGGGTTTTTACGTAAATCAGGTGaa > 1:2570392‑M1/43‑68 (MQ=255) cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa < 1:44940/68‑1 (MQ=35) cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaa < 1:40028615/67‑1 (MQ=35) cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaa < 1:17783837/67‑1 (MQ=35) cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaa > 1:56814493/1‑67 (MQ=35) catCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa < 1:19217346/68‑1 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:29730089/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:23529060/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:44873461/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:57761425/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:43136697/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACAAGGTGAAACTGa > 1:55938553/1‑67 (MQ=25) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTACGTAATCCACGTCAAACAGa > 1:18418187/1‑67 (MQ=11) atCTTTCTGCCACTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa > 1:41266014/1‑67 (MQ=25) tCTTTCTGCCCCTCCACAAGCAAAACCCCGCCGAAGAGGGTTTTTAAGTAAACCAGGTGAAACTGAcc < 1:45378962/68‑1 (MQ=11) tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc > 1:14397874/1‑68 (MQ=35) tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc < 1:30607687/68‑1 (MQ=35) ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGAt > 1:6340006/1‑68 (MQ=35) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg > 1:4991593/1‑67 (MQ=35) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg > 1:40832203/1‑67 (MQ=35) ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGCCCGATAAGcc > 1:10422905/1‑68 (MQ=25) ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc > 1:46702868/1‑68 (MQ=35) ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc > 1:27532599/1‑68 (MQ=35) cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCg > 1:26287815/1‑68 (MQ=35) cccTCCAAAAGC‑AAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc > 1:50979837/1‑66 (MQ=255) ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc > 1:42736156/1‑66 (MQ=35) ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGc > 1:30125425/1‑68 (MQ=37) ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGc < 1:34193175/68‑1 (MQ=37) cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctt > 1:18765995/1‑66 (MQ=40) aaaaaTCACAAACCCGCCGGACCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGctttc < 1:7089504/67‑1 (MQ=14) aaaaTCATAAACCCGCCGTATCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGctttct < 1:38863518/68‑1 (MQ=11) aaaaTCACAAACCCGCCGAACCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATACGCCGctttct < 1:57267439/68‑1 (MQ=11) aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttct < 1:20358162/68‑1 (MQ=255) aaaaGCAAAAACCCGCCGAACCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttct < 1:1691793/68‑1 (MQ=255) aaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttctt > 1:16199866/1‑68 (MQ=255) aatcAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt < 1:41840374/65‑1 (MQ=255) aaGCATAAACCCGCCGCAGCGGGTTATTACGTAAACCAGGTTAAACTGACCGATAAGCCGctttcttt < 1:38163388/68‑1 (MQ=14) aaGCACAAACCCGCCGTAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGctttcttt < 1:22534636/68‑1 (MQ=14) aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGcgttcttt > 1:55267020/1‑68 (MQ=255) aGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt > 1:52633316/1‑68 (MQ=40) gtaaaaaCCCGCCGAAGCGGGTTTTTACGTAATCCAGGGGAAACTGACCGATAAGCCGGTGTGTTTTg > 1:47508907/3‑68 (MQ=11) gCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg > 1:23274616/1‑68 (MQ=39) gCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg > 1:39063830/1‑68 (MQ=39) cataaaCCCGCCGCACCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTgg < 1:40480453/65‑1 (MQ=11) cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg > 1:48063015/1‑68 (MQ=37) taaaCCCGCCGCATCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt < 1:11571747/67‑1 (MQ=11) caaaCCCGCCGAACCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGt < 1:49584433/67‑1 (MQ=11) aaaaCCCGCCGAAGCGGGTTTTTTCGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt < 1:44993455/68‑1 (MQ=25) aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt < 1:9375276/68‑1 (MQ=35) aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGt < 1:28334022/68‑1 (MQ=35) aaCCCGCCGCAGCGGGTTATTACGTAAACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGtat < 1:32605139/68‑1 (MQ=11) aaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtat < 1:21221340/68‑1 (MQ=35) aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata > 1:18169546/1‑68 (MQ=35) aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata > 1:42390566/1‑68 (MQ=35) aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGtata > 1:24115421/1‑68 (MQ=255) cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata > 1:55006593/1‑67 (MQ=35) cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAg > 1:13675717/1‑68 (MQ=35) cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGtata > 1:6736402/1‑67 (MQ=25) cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGtata > 1:32203126/1‑67 (MQ=255) ccgccgTAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGc < 1:35392125/68‑1 (MQ=255) ccgccgCATCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGc < 1:54571302/68‑1 (MQ=255) gccgTAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgt < 1:15212111/68‑1 (MQ=255) gccgCAGCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgt < 1:26753842/68‑1 (MQ=14) gccgATGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgt < 1:12583521/68‑1 (MQ=255) gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgt < 1:45826339/68‑1 (MQ=40) gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgt < 1:4881649/68‑1 (MQ=40) gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgt < 1:15637363/68‑1 (MQ=40) gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgt < 1:8729918/68‑1 (MQ=40) gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgt < 1:31310828/68‑1 (MQ=40) gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgt < 1:1209448/68‑1 (MQ=40) ccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcg > 1:6978472/1‑66 (MQ=39) CGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGgaa < 1:48446390‑M1/68‑2 (MQ=255) GGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa > 1:39720756‑M1/1‑68 (MQ=255) ttattACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtc < 1:13019119/65‑1 (MQ=1) TTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGt > 1:19928197‑M1/1‑67 (MQ=255) TTTTTACGTAAATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTc > 1:14082981‑M1/1‑68 (MQ=255) TTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGt > 1:28578587‑M1/1‑67 (MQ=255) TTTTTACGTAAACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGt > 1:1063421‑M1/1‑67 (MQ=255) TTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTc > 1:37415532‑M1/1‑67 (MQ=255) TTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCa > 1:53076956‑M1/1‑68 (MQ=255) TTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTc > 1:29666562‑M1/1‑67 (MQ=255) TTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTc > 1:33166381‑M1/1‑67 (MQ=255) TTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCa > 1:33561966‑M1/1‑68 (MQ=255) TTTTACGTAAACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTc > 1:7627609‑M1/1‑67 (MQ=255) TTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTc > 1:51987438‑M1/1‑67 (MQ=255) TTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTc > 1:10854478‑M1/1‑67 (MQ=255) TTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTc > 1:14760867‑M1/1‑67 (MQ=255) aCGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGgcattca > 1:53814837/1‑68 (MQ=255) aaaTCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCt < 1:20761180/67‑1 (MQ=255) aaaCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCt < 1:50470846/67‑1 (MQ=40) ccAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATcat > 1:41380191/1‑63 (MQ=37) ccAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATGtt > 1:35926631/1‑65 (MQ=255) ccAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTAGTGGAAAGTCATTAATATTTca > 1:24707348/1‑67 (MQ=11) gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAAAGCGTCGTGGCCAGTCATTCATATTTCTTcc > 1:3618614/1‑67 (MQ=11) tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTCAGccc > 1:37188724/1‑67 (MQ=255) tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATGTTTCAGcccc > 1:27792720/1‑68 (MQ=255) tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGcc > 1:35590809/1‑66 (MQ=35) tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCCTTCATCTTTAAGcccc > 1:10015371/1‑68 (MQ=11) | AGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCT > CP009273/3263399‑3263533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |