| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,300,453 | T→G | 12.3% | N256T (AAC→ACC) | deaD ← | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,300,453 | 0 | T | G | 12.3% | 1774.0 / 59.8 | 742 | N256T (AAC→ACC) | deaD | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base T (334/307); new base G (98/2); total (433/309) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.66e-23 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 800 total aligned reads displayed.
GTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCT > CP009273/3300386‑3300518 | gTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGt > 1:9320980/1‑68 (MQ=255) tCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGtt > 1:46075945/1‑68 (MQ=255) tCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGtt > 1:43024331/1‑68 (MQ=255) aCCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGgttt > 1:14959149/1‑68 (MQ=255) ccGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGttttt > 1:39442098/1‑68 (MQ=255) gTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTgg > 1:189629/1‑68 (MQ=255) tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGt > 1:24836880/1‑67 (MQ=255) tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGt > 1:493279/1‑67 (MQ=255) tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGt > 1:38742115/1‑67 (MQ=255) aGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTaag > 1:39735106/1‑66 (MQ=255) gctgcgcTGTTGTAGCCGTTACTCTCAAGAGCTTCAGCCACTTCCAGAGTCGAGTTTTTGGTacgaa < 1:36758182/64‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTtcgaac > 1:27516072/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaac > 1:17192517/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaac > 1:2819901/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaac > 1:1520594/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaac > 1:9140813/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:40321733/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:47254773/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:34544201/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:30062844/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:11242982/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTacgaac > 1:54713477/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTacgaac > 1:43696461/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTacgaa > 1:7048665/1‑67 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGGTTTGGTacgaac > 1:5573183/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGGCGAGTTTTTTTTacgaac > 1:42548424/1‑68 (MQ=255) cgcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAcgaacgaa < 1:8165450/67‑1 (MQ=255) gcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGa < 1:1096085/68‑1 (MQ=255) gcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGa < 1:24254116/68‑1 (MQ=255) ttgtAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:20802802/58‑1 (MQ=255) tgtAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACTAATATaaa > 1:45069229/1‑66 (MQ=255) tgtAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAAGAACGAAGATAATc > 1:51042052/1‑68 (MQ=255) tgtAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTACGAACGAAGATAATc > 1:35562530/1‑68 (MQ=255) aGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgcc < 1:32347769/68‑1 (MQ=255) aGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGGACGAAAGAAGATAATcgcc > 1:34971847/1‑68 (MQ=255) aGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTTCGCACGAAGATAATcgcc > 1:33011165/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAGGCACCAAGATAATagccg > 1:13854609/1‑67 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:38047218/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:26786051/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:44807380/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:48866113/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccg > 1:52780940/1‑67 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTGTTTGGTTCGGACGAAGCTAAAagccg > 1:53289485/1‑67 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTTCGCACGAAGATAATcgccgc > 1:54080334/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTACGAACGAAGATAATcgccgc > 1:41358636/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGAGTTTTTTGTTAGAACGAACATAATctcc > 1:22750518/1‑66 (MQ=255) ctttACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACTAACGAAGATAATCGCCGCa < 1:57044027/66‑1 (MQ=255) cGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGt < 1:37410531/46‑1 (MQ=255) cGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCa < 1:33710260/68‑1 (MQ=255) cGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCa < 1:25922255/68‑1 (MQ=255) gTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCAt < 1:42148053/68‑1 (MQ=255) tCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACCAACGAAGATAATACCCGCATCaaaa > 1:10014148/1‑66 (MQ=255) agagCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCtt > 1:55406329/1‑67 (MQ=255) agagCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATAAAAAACtt > 1:46430992/1‑67 (MQ=255) gCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGCACGAAGATAATCGCCGCAACAAAAAATTcc > 1:12218058/1‑66 (MQ=255) gCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATAGCCGCAAAAAAAACTTCCGc > 1:31068708/1‑68 (MQ=255) gCTTCAGCCACTTCCAGAGTCGCGGTTTTTGTACGAACGAAGATAATAGCCGCATCAAAATAttac > 1:20421716/1‑64 (MQ=255) gCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTAAGCACGAAGATAATCTCCGCATAAAAATCTTCCGc > 1:21430424/1‑68 (MQ=255) ttCAGCCCCTTCCAGAGTCGCGTGTTTGGTACCCACCAACAAAATAGCCCCCACAAAAACATCCGcga > 1:18311548/1‑66 (MQ=255) ttCAGCCACTTCCAGAGTCGCGTTTTTGGTTCGAACGAAGATAATAGCCGCATAAAAATCTTCCGCtt > 1:13274114/1‑68 (MQ=255) ttCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCtt < 1:34188318/68‑1 (MQ=255) ttCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCtt < 1:50116497/68‑1 (MQ=255) ttCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCt < 1:49721882/67‑1 (MQ=255) ttCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCt < 1:5176282/67‑1 (MQ=255) ttCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCt < 1:7670529/67‑1 (MQ=255) ttCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATAGCCGCATCAAAATCTTCCGCt > 1:25313742/1‑67 (MQ=255) tCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTc < 1:10574539/68‑1 (MQ=255) tCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTc < 1:1169479/68‑1 (MQ=255) tCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTc < 1:19264081/68‑1 (MQ=255) tCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTc < 1:2364689/68‑1 (MQ=255) cAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTcc < 1:56835025/68‑1 (MQ=255) cAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTcc < 1:52224480/68‑1 (MQ=255) cAGCAACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTcc < 1:13064294/68‑1 (MQ=255) aGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCa > 1:46711098/1‑68 (MQ=255) cACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCa < 1:28823681/65‑1 (MQ=255) cACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCa < 1:27227209/65‑1 (MQ=255) cACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAg < 1:57505591/66‑1 (MQ=255) cACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGa < 1:57999597/68‑1 (MQ=255) cACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGa < 1:19801398/68‑1 (MQ=255) aCTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGaa < 1:408980/68‑1 (MQ=255) aCTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGaa < 1:14389556/68‑1 (MQ=255) aCTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGaa < 1:22077212/68‑1 (MQ=255) aCTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGaa < 1:44356148/68‑1 (MQ=255) aCTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGaa < 1:28557870/68‑1 (MQ=255) cTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGaa > 1:53780850/1‑67 (MQ=255) ttCCAGAGTCGCGTTTTTGGTACTAACGAAGATAATCCCCGCATCAAAATCTTCCGCTTCCAGGAAAc < 1:36275029/68‑1 (MQ=255) ttCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAAc < 1:16694621/68‑1 (MQ=255) ttCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAAc < 1:29326613/68‑1 (MQ=255) ttCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAAc < 1:48266285/68‑1 (MQ=255) ttCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAAc < 1:15893066/68‑1 (MQ=255) ttCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAAc < 1:15496692/68‑1 (MQ=255) ccAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGt < 1:56022727/68‑1 (MQ=255) ccAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGt < 1:6343843/68‑1 (MQ=255) ccAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGt < 1:23252052/68‑1 (MQ=255) ccAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTACGCTTCCAGGAAACGt > 1:16372266/1‑68 (MQ=255) ccAGAGTCGCGTTTTTGGTAACAACGAAAAAAAAAGCCCCCTCAAAATATTCAACTTACACGaaaagg > 1:17689693/1‑65 (MQ=255) agagTCGCGTTTTTTGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTa > 1:47725281/1‑67 (MQ=255) cgcgTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGt < 1:50462007/67‑1 (MQ=255) cgcgTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGt < 1:26516129/67‑1 (MQ=255) cgcgTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTg < 1:86811/68‑1 (MQ=255) | GTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCT > CP009273/3300386‑3300518 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |