| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,400 | A→G | 6.9% | S271S (AGT→AGC) | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,400 | 0 | A | G | 6.9% | 2302.4 / 46.2 | 753 | S271S (AGT→AGC) | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base A (395/288); new base G (49/3); total (462/291) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.38e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 754 total aligned reads displayed.
AGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAA > CP009273/3462336‑3462467 | aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTTCTg > 1:10016119/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTg > 1:39936718/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:36618888/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:51647332/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:40761106/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:41333946/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:42026216/1‑68 (MQ=255) ccAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGc > 1:53351225/1‑68 (MQ=255) gggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATc > 1:53896342/1‑68 (MQ=255) ggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATca > 1:29800296/1‑67 (MQ=255) ggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATca > 1:21502815/1‑67 (MQ=255) ggTTACCATACTGGCTGATATCTGTTGCCGTTCCCGCGCGAACATAACGACATGGGTTACTGGCaa > 1:43240773/1‑65 (MQ=255) gTTAGCATAGTGGCTGATTTCTTTTGACGTTGCCGCGCTAACATAACGCCATGGGTTACTGGCAt < 1:24494074/65‑1 (MQ=25) gTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt > 1:55554741/1‑68 (MQ=255) gTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt > 1:3486284/1‑68 (MQ=255) gTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt > 1:19924602/1‑68 (MQ=255) ttACCTTACTGGCTGATTTCTGTTGCCGTTGCCGCTCGATCATAACGCCATGGGTTACTGGCATCGt < 1:47753560/67‑1 (MQ=25) ttACCCTACTGTCTGATTTCTGTTGCCGTTGCCGCGCGAACATCACGCCATGGGTTACTGGCATCGt < 1:1632042/67‑1 (MQ=25) ttACCCTACTGACTGATTTCTGCTGCCGTTGCCGCTCGAACTACACGCCATGGGTTACTGGCATCGt < 1:24029970/67‑1 (MQ=21) ttACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCttt > 1:43949822/1‑68 (MQ=255) ttACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt < 1:16914567/67‑1 (MQ=255) ttACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt < 1:50935150/67‑1 (MQ=255) ttACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt < 1:10592201/67‑1 (MQ=255) tACCATACTGGATGATTTCTTTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGttt > 1:8197404/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCttttatt > 1:10221887/1‑65 (MQ=21) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCAtttctt > 1:2941481/1‑65 (MQ=18) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCAtttatt > 1:45392333/1‑65 (MQ=18) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGtttcgt > 1:33653772/1‑65 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct < 1:37670070/68‑1 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct < 1:25569860/68‑1 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:57016997/1‑68 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGTTTACAGGCACAGTtttctt > 1:55021841/1‑67 (MQ=25) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGc > 1:7913819/1‑56 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTCCTGGCATCGTtttctt > 1:580826/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttgttt > 1:35263266/1‑68 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:30913072/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:11867251/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:15183476/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:112399/1‑67 (MQ=255) aCTGGGTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTttgatttc > 1:56194834/1‑68 (MQ=21) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTTCTGGCATCATtttttttc > 1:17632932/1‑68 (MQ=18) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCTTttagtttc > 1:18747084/1‑68 (MQ=21) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctttc > 1:39376283/1‑68 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctttc > 1:1922582/1‑68 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttatttc > 1:26250428/1‑68 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTttgctttc > 1:21954970/1‑68 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTttaatttc > 1:30702331/1‑68 (MQ=255) cTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCg < 1:24854227/68‑1 (MQ=255) tGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGc > 1:26628396/1‑68 (MQ=255) tGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCCTTGTATTTCGc > 1:18116058/1‑68 (MQ=21) ggCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCATTTAGTTTCGCa > 1:9158950/1‑68 (MQ=18) cTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTGTTTCGCATc > 1:2887833/1‑68 (MQ=255) cTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATc > 1:22324112/1‑68 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:1359098/68‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:14438350/68‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:45094960/68‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:17363029/68‑1 (MQ=255) ttCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCGTTGGGTTTCGCATCAcccg > 1:34447524/1‑66 (MQ=11) ttCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATTGGGTTTCGCATCGccag > 1:41646167/1‑66 (MQ=14) tCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTgg < 1:12930209/68‑1 (MQ=255) tCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTgg < 1:32896139/68‑1 (MQ=255) tCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTgg < 1:32000384/68‑1 (MQ=255) cgttgccgCGCGAACAAAACGCCAGGGGTTACTGGCATCGATTTCTTACGCATCGCCTGGGCAATTTg < 1:18365787/68‑1 (MQ=255) gccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgc < 1:23431188/68‑1 (MQ=255) ccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgc > 1:50082628/1‑67 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:3575005/67‑1 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:38733096/67‑1 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:46990049/67‑1 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:48994724/67‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:14174004/68‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:28034725/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:34122798/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCAGCGCCTAccc < 1:5136500/67‑1 (MQ=255) tAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACccacca < 1:46232544/68‑1 (MQ=255) aaCGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAg < 1:50508330/68‑1 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:40278857/1‑68 (MQ=255) cGCCATGGGTTAATGGAATCTTCAGCTATCGCATCCCCTGGGCAATTTTCCGCGCCTACCCACCAGGc < 1:19506487/68‑1 (MQ=16) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg > 1:48446243/1‑68 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg < 1:38076994/68‑1 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg > 1:44874979/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTTTTTCGCATCGCCAGGTCATTTTGAAGCGCCTACCCACCAGGcat > 1:57432715/1‑66 (MQ=17) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:33355576/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATAGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:20346816/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTGATTTCGCATCGCCTGGGCAGTTAGACGCGGCTACCCACCAGGcct > 1:20983793/1‑66 (MQ=17) ccATGGGTTACAGGCATCTTTTGCTTTCGCATCACCAGGACACTTTGACGCGGCTACCCACCAGGcat > 1:22664704/1‑66 (MQ=2) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGt < 1:15756826/67‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:12386752/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:3237029/68‑1 (MQ=255) aTGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTc < 1:6760045/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:49404622/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:61103/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:7165119/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:7562836/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:43594780/68‑1 (MQ=255) tGGGTTACTCGCATCGTTTTCTTTCGCATCGCCTGGTCAATTTTCCGAGCCTACCCACCAGGCGTTCt < 1:27663423/68‑1 (MQ=255) gggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGACGCGCCTACCCACCAGGCAtttta > 1:52862662/1‑65 (MQ=37) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCATTTTGCAGCGGCTACCCACCAGGCGTTTTta > 1:4238632/1‑68 (MQ=25) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:8574224/63‑1 (MQ=255) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTtat > 1:52344248/1‑68 (MQ=255) aCTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTGTTATaaa > 1:27213487/1‑68 (MQ=255) | AGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAA > CP009273/3462336‑3462467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |