| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,437 | G→T | 7.1% | A259E (GCA→GAA) ‡ | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,437 | 0 | G | T | 7.1% | 3539.8 / 44.0 | 1130 | A259E (GCA→GAA) ‡ | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base G (240/775); new base T (2/113); total (242/888) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 1134 total aligned reads displayed.
CGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAAC > CP009273/3462370‑3462503 | cgttgccgCGCGAACAAAACGCCAGGGGTTACTGGCATCGATTTCTTACGCATCGCCTGGGCAATTTg < 1:18365787/68‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:38663250/68‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:32805229/68‑1 (MQ=255) aCATCACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAccc < 1:32327017/67‑1 (MQ=255) aCATATCGACATGGGTTACTGTCATCGTTTTCTTTCGCTTCGACTGGTAAATTTGCCTCGCCTACcca < 1:10366484/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:1865947/68‑1 (MQ=255) cGCCATGGGTTACTGGCATCTTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc < 1:45388399/68‑1 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:34493645/1‑68 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg < 1:55837320/68‑1 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg > 1:53505656/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:13710415/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATAGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:20346816/1‑68 (MQ=255) ccATGGGTTACTGGCATCATTTTCTTTCGCATCGCCAGGACAGTTAGCAGCGCCTACCCACCAGGcat > 1:55302967/1‑66 (MQ=14) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:42296577/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:17749420/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCACCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:38061086/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:7562836/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:4846607/68‑1 (MQ=255) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGAAGCGCCTACCCACCAGGCGttatta > 1:53028344/1‑68 (MQ=37) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTtat > 1:52344248/1‑68 (MQ=255) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta < 1:23208368/67‑1 (MQ=255) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGACGCGCCTACCCACCAGGCGTTCTta < 1:22316366/67‑1 (MQ=255) ttACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTtata > 1:13026675/1‑68 (MQ=255) ttACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTtata > 1:2417294/1‑68 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGGAGAGCCTACCCACCAGGCTTTGTTATAAAtt > 1:39399350/1‑67 (MQ=25) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCTTTCTTATaaag > 1:37664397/1‑66 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAAt > 1:3107016/1‑67 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAAt > 1:47622007/1‑67 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAAt > 1:57337002/1‑67 (MQ=255) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATg > 1:35378035/1‑67 (MQ=255) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTATTATaaaag > 1:2271291/1‑65 (MQ=255) aTCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgc < 1:44498855/68‑1 (MQ=255) tctttttCATTCGACTCGCCTTGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:33163823/65‑1 (MQ=37) tCGTTTTCTTTCGCATCGCGTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:27284900/68‑1 (MQ=255) tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:16394002/68‑1 (MQ=255) tCGTTTTATATCGTACCCCCTGGGCAATTTTAAGAGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:4490291/68‑1 (MQ=12) tttttCTTTCGGAACGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:19129138/67‑1 (MQ=39) tttttCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:2100588/67‑1 (MQ=255) tttttCTTTCGAATCGCCTGTGCAATTTGCCGCGCCTACCCCCCAGGCGTTCTTATAAATGCCGCCGt < 1:46866787/67‑1 (MQ=255) ttattctttcGTAGCGCGTGGGCAATTTTAAGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:5055191/65‑1 (MQ=18) gtcttctttcGCATCCCCTGGGCAATCTTACGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:49623021/65‑1 (MQ=21) gTTTTCTTTCGGATCGCCTGGGCAATTTTACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:29256154/68‑1 (MQ=38) gTTTTCTTTCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:31706925/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:10670205/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:56947564/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:43208656/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:46071994/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:46426242/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:40901142/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:23898504/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:24698065/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:10037143/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:62005/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:33429458/68‑1 (MQ=255) gTTTTCTTGCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:54211021/68‑1 (MQ=255) ttttCTTTCGCATCTCCTTGTCAATTTTAAGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:51094191/67‑1 (MQ=25) ttttCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGtt > 1:48970216/1‑68 (MQ=255) ttttCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCCTTATTATAAATGCCGCCGtt > 1:50192153/1‑68 (MQ=255) ttttCTTTCGCATCGCCTGGGCAATTTGCCGCCCCTACCCACCAGGCGTTATTATAAATGGAGCCGtt > 1:57953378/1‑68 (MQ=255) tcttcgttcgTATCCCCTGGGCAATATTTCGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:52654616/65‑1 (MQ=18) ttctttcGCTTCTACTGTGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:43779827/68‑1 (MQ=38) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:19616294/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:25755576/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:16886034/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat > 1:29854372/1‑68 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:15754569/68‑1 (MQ=255) tctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtata > 1:53822293/1‑68 (MQ=255) ctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAg < 1:26658727/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:47986478/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:35027397/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:40422604/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:21451365/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:28094053/68‑1 (MQ=255) cGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATaa < 1:36502710/68‑1 (MQ=255) accGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:2182746/66‑1 (MQ=255) aTCGCCTGGGCAATTTGGCTCGCCTACCCACCAGTCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:28822870/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:4140378/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:30828393/68‑1 (MQ=255) aTCGCCTGGGCAATCTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:11451397/68‑1 (MQ=255) cGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAc < 1:35836703/68‑1 (MQ=255) cGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAc < 1:34003226/68‑1 (MQ=255) gCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAcc < 1:12059493/68‑1 (MQ=255) gCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAcc < 1:27680004/68‑1 (MQ=255) ccTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCa > 1:30452546/1‑68 (MQ=255) cggggCAATCTTCCGCGCCAACCCAACAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:930458/66‑1 (MQ=255) cTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:8836555/68‑1 (MQ=255) cTGGGCAATTTTACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:40054928/68‑1 (MQ=255) cTGGGCAATTTGCCGCGGCTACCCACCAGGCGTTCTTTTAAATGCCGCCGTTATAGATAACGTACCAt < 1:56369353/68‑1 (MQ=39) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:26319158/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:51622033/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:19912226/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:1440742/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:12420270/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:8174811/68‑1 (MQ=255) gggCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGt < 1:42768706/68‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:17249010/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:38944293/67‑1 (MQ=255) gCAATTTGCAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:15070524/67‑1 (MQ=255) gCAATTTGCAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:7202751/67‑1 (MQ=255) tGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAgaagaa > 1:25352942/1‑67 (MQ=255) | CGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAAC > CP009273/3462370‑3462503 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |