| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,676 | A→T | 7.9% | A179A (GCT→GCA) | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,676 | 0 | A | T | 7.9% | 3264.0 / 74.6 | 990 | A179A (GCT→GCA) | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base A (536/358); new base T (11/85); total (547/443) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.94e-21 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 1000 total aligned reads displayed.
CCGGTGAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTAGAAG > CP009273/3462609‑3462743 | gAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCgg < 1:52989882/67‑1 (MQ=255) aaGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGc > 1:31487584/1‑67 (MQ=255) aGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCa < 1:19019947/67‑1 (MQ=255) aGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCAc > 1:11881677/1‑68 (MQ=255) aGGATGTGGTGTTGAAGTACAGTAGTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCa < 1:12168659/67‑1 (MQ=255) gCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa > 1:2455678/1‑68 (MQ=255) gCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa > 1:27080096/1‑68 (MQ=255) gCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa > 1:43888403/1‑68 (MQ=255) cTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa > 1:19470376/1‑67 (MQ=255) cTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa > 1:52568007/1‑67 (MQ=255) cTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa < 1:12677412/67‑1 (MQ=255) cTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa > 1:58031496/1‑67 (MQ=255) tgtgGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGaa < 1:27899114/67‑1 (MQ=255) tgtgGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAAc < 1:42299418/68‑1 (MQ=255) tgtgGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAAc < 1:31974972/68‑1 (MQ=255) gtggtgCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACa < 1:40803177/68‑1 (MQ=255) ggtgCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATa > 1:17536716/1‑68 (MQ=255) gCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAAc > 1:19689659/1‑67 (MQ=255) gCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACg < 1:772244/68‑1 (MQ=255) tGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGcc > 1:14475329/1‑68 (MQ=255) tGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGcc > 1:22983267/1‑68 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCAt < 1:17016002/67‑1 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:16630286/1‑68 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:43393526/1‑68 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:41998091/1‑68 (MQ=255) tACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAAc < 1:6439742/55‑1 (MQ=255) tACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATggg > 1:55439442/1‑68 (MQ=255) tACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATggg > 1:38661837/1‑68 (MQ=255) aCAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATggg < 1:22376188/67‑1 (MQ=255) aCAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATggg < 1:48344650/67‑1 (MQ=255) aCAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATgg < 1:36606998/66‑1 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:41389119/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:26407686/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:21408825/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:39006148/1‑67 (MQ=255) ggACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:47438100/1‑66 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:51234363/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:35924129/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:37095998/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:37702487/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:49093581/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:13589219/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:42721413/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:13955226/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:8455937/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:38348081/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:1544389/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:28575517/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:23686707/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCAAGGGTTGCTg > 1:45679924/1‑68 (MQ=255) tgtgGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGc < 1:5733787/68‑1 (MQ=255) gtgGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGca < 1:4474143/68‑1 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc > 1:24137186/1‑68 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc > 1:34817691/1‑68 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc > 1:34160456/1‑68 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc > 1:30716076/1‑68 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc > 1:47734944/1‑68 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc > 1:11227854/1‑68 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc > 1:50226862/1‑68 (MQ=255) aTTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatcat > 1:20465347/1‑68 (MQ=255) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt > 1:54710712/1‑66 (MQ=255) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt > 1:1158950/1‑66 (MQ=255) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt > 1:30219328/1‑66 (MQ=255) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt > 1:13242176/1‑66 (MQ=255) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGc > 1:54028233/1‑68 (MQ=255) ggTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTTCttt > 1:48736292/1‑68 (MQ=255) gagaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCAt > 1:9534268/1‑67 (MQ=255) tgaTTTCTGTTGCCGTTGCCGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:25599155/67‑1 (MQ=14) tgaTTTCTGTTGCCGTAGCCGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATc < 1:33351710/66‑1 (MQ=17) tgaTTTCTGTTGCCGTAGCCGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:33763280/67‑1 (MQ=18) agaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATc > 1:51590152/1‑67 (MQ=255) gaTTTCGGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:46543466/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa > 1:53477712/1‑67 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:25136432/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:7245486/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCACCa < 1:56422689/67‑1 (MQ=255) tttCGGTTGCCGTAGCTGTACGAACATAACGGCATGGGTTGCTGGCATCATTGCTTTTCGCATCAcc < 1:29541770/67‑1 (MQ=25) tttCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCAcc < 1:10333693/67‑1 (MQ=255) tttCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCAcc < 1:20105982/67‑1 (MQ=255) cGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGAc > 1:403378/1‑68 (MQ=255) tGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCCTTGCTTTTCGCATCACCCGGACAg < 1:56169098/67‑1 (MQ=21) tGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:20963060/67‑1 (MQ=255) tGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:56953779/67‑1 (MQ=255) tGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:28202637/68‑1 (MQ=255) tGCCGTTGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:32496894/67‑1 (MQ=255) tGCCGTTGCCGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:9040391/67‑1 (MQ=18) tGCCGTTGCCGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:39757376/67‑1 (MQ=18) tGCCGTTGCCGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:14833035/67‑1 (MQ=18) tGCCGTTGCCGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:21911719/67‑1 (MQ=18) tGCCGTAGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:35529457/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:29121194/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:17960207/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:45262322/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:49735617/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:1015013/68‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:18364759/68‑1 (MQ=255) gCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGtt < 1:55192407/68‑1 (MQ=255) gCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGtt > 1:50552757/1‑68 (MQ=255) gTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTaga < 1:15899460/68‑1 (MQ=255) gTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTaga < 1:46950989/68‑1 (MQ=255) | CCGGTGAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTAGAAG > CP009273/3462609‑3462743 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |