| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,076,055 | T→G | 6.0% | Q22P (CAG→CCG) | fdoH ← | formate dehydrogenase‑O, Fe‑S subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,076,055 | 0 | T | G | 6.0% | 2161.7 / 18.1 | 906 | Q22P (CAG→CCG) | fdoH | formate dehydrogenase‑O, Fe‑S subunit |
| Reads supporting (aligned to +/- strand): ref base T (308/522); new base G (59/8); total (375/531) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-16 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 912 total aligned reads displayed.
GCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATG > CP009273/4075989‑4076122 | tACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgc < 1:46111927/68‑1 (MQ=255) aGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGcgcg > 1:11530468/1‑68 (MQ=255) aGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGcgcg > 1:1388380/1‑68 (MQ=255) aGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCCGGGGcgcg > 1:47115690/1‑68 (MQ=255) aGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGACTGAGGcgcg > 1:30133649/1‑68 (MQ=255) gCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGCGGCGCgg > 1:18892400/1‑68 (MQ=255) gCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCgg > 1:54757028/1‑68 (MQ=255) gCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCgg > 1:10036285/1‑68 (MQ=255) gCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCgg > 1:55279397/1‑68 (MQ=255) gCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGCCGCGCgg > 1:43171986/1‑68 (MQ=37) gCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGCgg > 1:30979717/1‑68 (MQ=255) gCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGCgg > 1:57964699/1‑68 (MQ=255) gCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCCCCTGAGGCGCgg > 1:40951154/1‑68 (MQ=255) cGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCgggg < 1:33069828/68‑1 (MQ=255) cGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCgggg < 1:379485/68‑1 (MQ=255) gATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCggggg < 1:17896526/68‑1 (MQ=255) tACAGCTGGTAACGGCGATGAGCTTCGCCACTTCTTCCTGGCAGACCCGCGCCTGAGGCGCGGGGGt < 1:55905655/67‑1 (MQ=255) cAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCa > 1:40141914/1‑67 (MQ=255) cAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAg > 1:34161939/1‑68 (MQ=255) cAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAg > 1:12027362/1‑68 (MQ=255) cAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGCGGGGGTCa > 1:48769256/1‑67 (MQ=255) cAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGCGGGGGTCAg > 1:51898843/1‑68 (MQ=255) aGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGAGGCGCGGGGGTCAg > 1:26300251/1‑67 (MQ=255) ggtggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGCGGCGCGGGGGTCAGAc > 1:57151139/1‑68 (MQ=255) ggtggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAgc > 1:23443725/1‑66 (MQ=255) ggtggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGa > 1:33716295/1‑67 (MQ=255) ggtggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGa > 1:50308173/1‑67 (MQ=255) ggtggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGa > 1:18483485/1‑67 (MQ=255) ggtggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGa > 1:14295065/1‑67 (MQ=255) ggtggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGGCAGa > 1:3441187/1‑67 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGa < 1:12695028/65‑1 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGa < 1:19275682/65‑1 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGa < 1:40544359/65‑1 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGAc < 1:15099332/66‑1 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGAc < 1:7246880/66‑1 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGAc < 1:53685016/66‑1 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGAc < 1:46537583/66‑1 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCg > 1:23691194/1‑68 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCg > 1:21998968/1‑68 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCCCCAGAGGCGCGGGGGGCAGACCg > 1:1566104/1‑68 (MQ=255) tAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTa > 1:52426635/1‑68 (MQ=255) aCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGGCGGAGGCGCGGGGGTCAGAACGTTAGt > 1:879622/1‑68 (MQ=255) aCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGCGGCGCGGGGGGCAGACCGTTAg > 1:20041656/1‑67 (MQ=255) aCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAg > 1:32112690/1‑67 (MQ=255) aCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAg > 1:6411686/1‑67 (MQ=255) aCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAg > 1:27235704/1‑67 (MQ=255) aCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGt < 1:3056790/68‑1 (MQ=255) aCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCGGGGGCGCGGGGGTCAGCCCGTTAg > 1:50866346/1‑67 (MQ=255) aCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCAGAGGCGCGGGGGTCAGACCGTTatt > 1:2703970/1‑66 (MQ=255) gTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTc > 1:31423334/1‑67 (MQ=255) gAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAac < 1:29614322/68‑1 (MQ=255) aGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAacg > 1:20778608/1‑68 (MQ=255) aGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAacg > 1:56788318/1‑68 (MQ=255) aGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCCGGGGTCAGACCGTTAGTCGCGGAac > 1:25574649/1‑67 (MQ=255) ttCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAacgacg > 1:6662129/1‑68 (MQ=255) ttCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAacgac < 1:7603930/67‑1 (MQ=255) tCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAAcgacga < 1:45708125/68‑1 (MQ=255) tCGCCACATCTTCCTGGAAGGCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAAcgacga < 1:49739079/68‑1 (MQ=255) cGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGaa < 1:27747478/68‑1 (MQ=255) gCCACTTCTTCCTGGAAGGCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAAt < 1:48180304/68‑1 (MQ=255) ccACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGCCCGTTAGTCGCGGAACGACGAATg > 1:22601376/1‑68 (MQ=255) cACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGa < 1:51446805/68‑1 (MQ=255) cACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGa < 1:37347654/68‑1 (MQ=255) cACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGa < 1:3673367/68‑1 (MQ=255) cACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGa < 1:35856410/68‑1 (MQ=255) cACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGa < 1:6288956/68‑1 (MQ=255) cACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGa < 1:3254415/68‑1 (MQ=255) cACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGa > 1:16474187/1‑68 (MQ=255) cACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGa < 1:49064313/68‑1 (MQ=255) cttcttCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGata < 1:14786638/68‑1 (MQ=255) ttcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGatat < 1:39756823/68‑1 (MQ=255) ttcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGatat < 1:9308658/68‑1 (MQ=255) ttcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGatat < 1:3908998/68‑1 (MQ=255) tcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATc < 1:43801738/68‑1 (MQ=255) tcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATc < 1:19691299/68‑1 (MQ=255) tcttcCTGGAACTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATc < 1:53011174/68‑1 (MQ=255) cttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCt < 1:56482302/68‑1 (MQ=255) aaGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGAtt < 1:4225540/67‑1 (MQ=255) aaGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTg > 1:34861752/1‑68 (MQ=255) aGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGa < 1:21632803/68‑1 (MQ=255) aGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGa < 1:38588616/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:57611389/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:8068018/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:36237864/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:54147499/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:47596929/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:45078093/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:28447867/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:24925858/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:24413365/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:17501566/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:16146070/68‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGc < 1:13432879/68‑1 (MQ=255) gcgcCGGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGcc < 1:10509608/68‑1 (MQ=255) gcCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTga < 1:42747490/54‑1 (MQ=255) gcCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCa < 1:16987466/67‑1 (MQ=255) ccTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCata > 1:11142325/1‑68 (MQ=255) cTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCata < 1:44578888/67‑1 (MQ=255) cTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCata < 1:4162029/67‑1 (MQ=255) cTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCata < 1:28856826/67‑1 (MQ=255) | GCTTTACAGCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATG > CP009273/4075989‑4076122 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |