| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,106,438 | A→G | 100% | I238T (ATT→ACT) | glpK ← | glycerol kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,106,438 | 0 | A | G | 98.4% | 2879.9 / 17.6 | 788 | I238T (ATT→ACT) | glpK | glycerol kinase |
| Reads supporting (aligned to +/- strand): ref base A (8/5); new base G (344/429); total (353/435) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 788 total aligned reads displayed.
CTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTC > CP009273/4106373‑4106502 | ttcttTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑c > 1:52450368/1‑68 (MQ=255) cttTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑cgc > 1:31257172/1‑68 (MQ=255) cttTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑cgc > 1:46774332/1‑68 (MQ=255) ttCACGCACAACTGACCAAACCGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGt > 1:13631514/1‑68 (MQ=255) ttCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGt > 1:23446337/1‑68 (MQ=255) ttCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGt > 1:10078822/1‑68 (MQ=255) ttCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGt > 1:31864352/1‑68 (MQ=255) ttCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGt > 1:52780593/1‑68 (MQ=255) ttCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGt > 1:51570447/1‑68 (MQ=255) cACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTg > 1:43611487/1‑67 (MQ=255) caACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCtt < 1:12184786/68‑1 (MQ=255) aCTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCttt > 1:26790592/1‑67 (MQ=255) aCTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCttt > 1:46377040/1‑67 (MQ=255) aCTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCttt > 1:44212433/1‑67 (MQ=255) aCTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCttt > 1:18183083/1‑67 (MQ=255) aCTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCttt > 1:16666575/1‑67 (MQ=255) aCTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCttt > 1:40533710/1‑67 (MQ=255) aCTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATA‑‑CGCGTGCCGCCttt > 1:22411781/1‑67 (MQ=255) tGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTgcc < 1:2021811/68‑1 (MQ=255) ccAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTgccgcc > 1:17816628/1‑68 (MQ=255) ccAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTgccgcc > 1:19440142/1‑68 (MQ=255) ccAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTgccgcc > 1:14931871/1‑68 (MQ=255) ccAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTgccgc < 1:15524782/67‑1 (MQ=255) ccAAACAGCGCGGCCTGCTCGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCCTGCCGCCTTTgccgcc > 1:33622012/1‑68 (MQ=255) aaaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCaa > 1:9641959/1‑68 (MQ=255) aaaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCaa > 1:30636742/1‑68 (MQ=255) aaaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCaa > 1:54727225/1‑68 (MQ=255) accAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAAt < 1:14622710/66‑1 (MQ=255) aaCAGCGCGGCCTGCTGGTCACCGGGGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAAt < 1:38017401/68‑1 (MQ=255) aaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAAt > 1:35178706/1‑68 (MQ=255) aaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAAt < 1:3307215/68‑1 (MQ=255) aaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAAt < 1:38387237/68‑1 (MQ=255) aaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAAt < 1:41995144/68‑1 (MQ=255) aaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAAt < 1:42608196/68‑1 (MQ=255) aaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAAt > 1:17400866/1‑68 (MQ=255) aaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAAt < 1:51027554/68‑1 (MQ=255) aaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAAt > 1:25758018/1‑68 (MQ=255) aCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATg > 1:32779915/1‑68 (MQ=255) aCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATg > 1:44770889/1‑68 (MQ=255) aCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATg > 1:21863427/1‑68 (MQ=255) cAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGt > 1:48434459/1‑68 (MQ=255) cAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGt > 1:55368791/1‑68 (MQ=255) cAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCAGCCAATGt > 1:39297785/1‑68 (MQ=255) gcgGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTa < 1:36259587/66‑1 (MQ=255) gcgGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGt < 1:57802646/68‑1 (MQ=255) gcgGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGt < 1:732985/68‑1 (MQ=255) gcgGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGt < 1:35809512/68‑1 (MQ=255) ctggtgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:36804255/68‑1 (MQ=255) ctgctgGTCACCGGCTATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCCTTGCCGCCAATGTTAGTCTGAc < 1:53686234/68‑1 (MQ=255) ctgctgGTCACCGGCGATCCGGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:54332871/68‑1 (MQ=255) ctgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGa < 1:8765286/67‑1 (MQ=255) ctgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:18428608/68‑1 (MQ=255) ctgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:47923189/68‑1 (MQ=255) ctgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:49323101/68‑1 (MQ=255) ctgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:49741548/68‑1 (MQ=255) ctgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:45040126/68‑1 (MQ=255) ctgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:24772322/68‑1 (MQ=255) ctgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:44519910/68‑1 (MQ=255) ctgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:26514268/68‑1 (MQ=255) ctgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:50832552/68‑1 (MQ=255) ctgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:20601437/68‑1 (MQ=255) ctgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:51901103/68‑1 (MQ=255) ctgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:5332998/68‑1 (MQ=255) ctgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:27843736/68‑1 (MQ=255) ctgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:1419607/68‑1 (MQ=255) ctgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAc < 1:38760693/68‑1 (MQ=255) tgctgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGAcc < 1:25114146/68‑1 (MQ=255) tgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGt < 1:6483201/67‑1 (MQ=255) tgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGt < 1:24098934/67‑1 (MQ=255) tgGTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGt < 1:34441503/67‑1 (MQ=255) ggTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGtat > 1:34217770/1‑68 (MQ=255) ggTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGtat > 1:57378548/1‑68 (MQ=255) ggTCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGtat > 1:10971537/1‑68 (MQ=255) tCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGtat > 1:56638068/1‑66 (MQ=255) tCACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAAAGTTAGTCTGACCGtata < 1:50246696/67‑1 (MQ=255) cACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATTTTAGTCTGACCGTATACt < 1:11493458/68‑1 (MQ=255) cACCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACt < 1:37396218/68‑1 (MQ=255) aCCGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACt > 1:27532301/1‑67 (MQ=255) ccGGCGATCCCGGAGATTGGAGTACGCGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACt < 1:16123452/68‑1 (MQ=255) cGGCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCg < 1:27101843/68‑1 (MQ=255) ggCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGCCCGTTTACTTCgg > 1:55994432/1‑68 (MQ=255) ggCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCgg > 1:22891410/1‑68 (MQ=255) ggCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCgg > 1:32508702/1‑68 (MQ=255) ggCGATCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCgg > 1:12758207/1‑68 (MQ=255) tCCCGGAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGgaaga < 1:30049524/67‑1 (MQ=255) ggAGATTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAacgac < 1:29195949/68‑1 (MQ=255) cgagaATGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAacgac < 1:28370695/67‑1 (MQ=255) agaTTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAacgacg < 1:6007539/67‑1 (MQ=255) agaTTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGc > 1:39644182/1‑68 (MQ=255) agaTTGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAAGGTTAGTCTGACCGTATACTTCGGAAGAACGACGc > 1:10655628/1‑68 (MQ=255) ttGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCAc < 1:41563296/67‑1 (MQ=255) ttGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCAc < 1:18889970/67‑1 (MQ=255) ttGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCAc < 1:42958320/67‑1 (MQ=255) ttGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACt < 1:21191703/68‑1 (MQ=255) ttGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACt < 1:45690231/68‑1 (MQ=255) ttGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACt < 1:9229320/68‑1 (MQ=255) tGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACtt < 1:41085609/68‑1 (MQ=255) tGGAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACtt < 1:8090707/68‑1 (MQ=255) ggAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACt > 1:697560/1‑66 (MQ=255) ggAGTA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTc < 1:39974273/68‑1 (MQ=255) | CTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATA‑‑CGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTC > CP009273/4106373‑4106502 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |