| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,172,748 | C→T | 91.8% | H526Y (CAC→TAC) | rpoB → | RNA polymerase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,172,748 | 0 | C | T | 91.8% | 2799.3 / 151.8 | 832 | H526Y (CAC→TAC) | rpoB | RNA polymerase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base C (41/27); new base T (444/319); total (485/347) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 888 total aligned reads displayed.
AGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGT > CP009273/4172681‑4172812 | agagTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGc > 1:17461609/1‑68 (MQ=255) agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAAGCCGCTGTCTGAGATTACGtac > 1:53585205/1‑68 (MQ=255) agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGtac > 1:40581754/1‑68 (MQ=255) agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGtac > 1:52972729/1‑68 (MQ=255) agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGtac > 1:36955234/1‑68 (MQ=255) agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGtac > 1:35951059/1‑68 (MQ=255) agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGtac > 1:13879006/1‑68 (MQ=255) agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGtac > 1:16664953/1‑68 (MQ=255) agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATAACGtac > 1:50262921/1‑68 (MQ=255) tcttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACaaa < 1:8478179/68‑1 (MQ=255) ttcGGTTCCAGCCAGCTGTCTCAGTTTATTGACCAGATCAACCCGCTGTCTGAGATTACGTACAAAcg < 1:6258847/68‑1 (MQ=255) ttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAAcg < 1:16001955/68‑1 (MQ=255) ttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAAcg < 1:52571084/68‑1 (MQ=255) ttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAAcg < 1:47277931/68‑1 (MQ=255) ttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAAcg < 1:51852863/68‑1 (MQ=255) cGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAAcgtc < 1:14630853/68‑1 (MQ=255) ggTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAAcgtcg > 1:19451195/1‑68 (MQ=255) tCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTa < 1:6661688/67‑1 (MQ=255) tCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTa > 1:20818852/1‑67 (MQ=255) cagccagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGGAtct < 1:23401520/68‑1 (MQ=255) ccagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTcc > 1:49912128/1‑67 (MQ=255) ccagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTcc > 1:30142722/1‑67 (MQ=255) cagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTAtctc < 1:47954423/65‑1 (MQ=255) gcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGc > 1:18335191/1‑66 (MQ=255) gcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGc > 1:2606655/1‑66 (MQ=255) tGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTc > 1:43751026/1‑68 (MQ=255) tGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTc > 1:1254206/1‑68 (MQ=255) gTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCg < 1:50866256/68‑1 (MQ=255) tctcAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCg < 1:7778437/67‑1 (MQ=255) ctcAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGc < 1:5922476/68‑1 (MQ=255) ctcAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGc < 1:41510245/68‑1 (MQ=255) ctcAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGc < 1:42602455/68‑1 (MQ=255) ctcAGTTTATGGACCAGAACAACCCGCGGGCTGAGATTACGTACAAACGTCGTATCTCCGCACTCgg < 1:20694510/67‑1 (MQ=255) tcAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGcc < 1:7495402/68‑1 (MQ=255) tttATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAg > 1:24712566/1‑67 (MQ=255) tttATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAg > 1:21210323/1‑67 (MQ=255) tttATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAg > 1:54649840/1‑67 (MQ=255) tttATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAg > 1:38010936/1‑67 (MQ=255) tttATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAg > 1:38369650/1‑67 (MQ=255) tttATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAg > 1:44840751/1‑67 (MQ=255) tttATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAg > 1:26954996/1‑67 (MQ=255) tttATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAg > 1:47517389/1‑67 (MQ=255) tATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCg > 1:15339026/1‑68 (MQ=255) aTGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg < 1:38719210/68‑1 (MQ=255) aTGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg < 1:33636042/68‑1 (MQ=255) aTGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg < 1:3757057/68‑1 (MQ=255) aTGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg < 1:57653336/68‑1 (MQ=255) aTGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCACGCgg < 1:31188052/68‑1 (MQ=255) tGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg > 1:23055791/1‑67 (MQ=255) tGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg > 1:57193351/1‑67 (MQ=255) tGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg > 1:453957/1‑67 (MQ=255) gACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg > 1:56073805/1‑65 (MQ=255) gACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg > 1:51543180/1‑65 (MQ=255) gACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg > 1:19053186/1‑65 (MQ=255) gACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg > 1:2723680/1‑65 (MQ=255) gACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg > 1:46958367/1‑65 (MQ=255) ccAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTg > 1:9229289/1‑67 (MQ=255) ccAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTg > 1:32369227/1‑67 (MQ=255) ccAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGa < 1:56857862/68‑1 (MQ=255) cAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGAc < 1:40115210/68‑1 (MQ=255) cAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGAc < 1:16912623/68‑1 (MQ=255) acaacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGAcc < 1:54074929/65‑1 (MQ=255) acaacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGAcc < 1:29568323/65‑1 (MQ=255) acaacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGAcc < 1:28871037/65‑1 (MQ=255) acaacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt > 1:3184111/1‑68 (MQ=255) acaacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt > 1:28092516/1‑68 (MQ=255) acaacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt > 1:23991073/1‑68 (MQ=255) acaacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt > 1:22634827/1‑68 (MQ=255) acaacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt > 1:20235411/1‑68 (MQ=255) acaacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGCTCTGCCCCGt > 1:11391381/1‑68 (MQ=255) aacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGa > 1:35411142/1‑68 (MQ=255) aacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGa > 1:24341471/1‑68 (MQ=255) ccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACg > 1:9651186/1‑68 (MQ=255) ccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACg > 1:45978885/1‑68 (MQ=255) ccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACg > 1:43347232/1‑68 (MQ=255) ccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACg > 1:41936710/1‑68 (MQ=255) ccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACg > 1:41645609/1‑68 (MQ=255) ccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACg > 1:36495542/1‑68 (MQ=255) ccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACg > 1:21779671/1‑68 (MQ=255) ccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACg > 1:5502777/1‑68 (MQ=255) gCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGt > 1:25601127/1‑67 (MQ=255) cTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGc > 1:39159404/1‑68 (MQ=255) cTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAgg < 1:2769807/67‑1 (MQ=255) cTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGc < 1:11882533/68‑1 (MQ=255) cTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGc < 1:1294645/68‑1 (MQ=255) aTTACGTACAAACGTCGTATTTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGa > 1:10343696/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:871116/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:11045533/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:14458514/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:33195214/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:34602728/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:34896810/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:55419411/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:39723784/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:41115033/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:44332368/1‑68 (MQ=255) aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa > 1:48861870/1‑68 (MQ=255) aCGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGt < 1:10049789/68‑1 (MQ=255) aCGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGt < 1:46343057/68‑1 (MQ=255) aCGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGt < 1:49493386/68‑1 (MQ=255) | AGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGT > CP009273/4172681‑4172812 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |