BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	692,040	G→A	6.3%	noncoding (33/75 nt)	glnV ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	692,040	0	G	A	6.3%	2539.1 / 87.3	873	noncoding (33/75 nt)	glnV	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base G (540/244); new base A (12/40); total (565/308)
Fisher's exact test for biased strand distribution p-value = 7.17e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 890 total aligned reads displayed.

TACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCG  >  CP009273/691973‑692105

   aaGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                 >  1:32507586/1‑67 (MQ=255)

   aaGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                 >  1:36895486/1‑67 (MQ=255)

   aaGGTAAGCGGCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                                                                <  1:46073685/68‑1 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                              >  1:17437697/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                              >  1:24249979/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                              >  1:28440546/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                              >  1:22252087/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                              >  1:31033794/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                              >  1:39772693/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                              >  1:47957825/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                              >  1:10384607/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                              >  1:918862/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                              >  1:6645829/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                              >  1:55783242/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCACATTcc                                                              >  1:23609592/1‑68 (MQ=255)

       tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                            >  1:53422700/1‑68 (MQ=255)

       tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                            >  1:39522208/1‑68 (MQ=255)

       tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                            >  1:20720126/1‑68 (MQ=255)

        cgcgaccttttttaatcTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGTATCAAAATCCGGt                                                           <  1:32155337‑M2/51‑1 (MQ=255)

         aGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                           >  1:12413535/1‑67 (MQ=255)

          ggagcttttttaaatTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                         <  1:51306208‑M2/53‑1 (MQ=255)

           gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGc                                                         <  1:31639224‑M2/53‑1 (MQ=255)

           gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                        <  1:29134162‑M2/54‑1 (MQ=255)

           gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                         <  1:19887171‑M2/53‑1 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                          >  1:9714011/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                          >  1:33573691/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                          >  1:42342332/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                          >  1:47214909/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                          >  1:16178570/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                          >  1:15850539/1‑66 (MQ=255)

            gTCTTGTATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                       >  1:44392168/1‑68 (MQ=255)

            gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                       >  1:11937183/1‑68 (MQ=255)

             tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                      >  1:41783451/1‑68 (MQ=255)

             tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAACCGGTGCCtt                                                      >  1:5507291/1‑68 (MQ=255)

              cttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                      >  1:18712154‑M2/12‑67 (MQ=255)

               ttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                      <  1:58088261‑M2/56‑1 (MQ=255)

                tttttaattTGTCTGGGGGACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                   <  1:25831133‑M2/59‑1 (MQ=255)

                tttttaattTGGCTGGTGTACGAGGATTCGAAGCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                   <  1:45776284‑M2/59‑1 (MQ=255)

                tttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                   <  1:5065725‑M2/59‑1 (MQ=255)

                 ttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                         <  1:7529672‑M2/53‑1 (MQ=255)

                  tttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                                 <  1:43374544‑M2/61‑1 (MQ=255)

                   ttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                >  1:4511671‑M2/7‑68 (MQ=255)

                   aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                >  1:5302599/1‑68 (MQ=40)

                   aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                >  1:17134450/1‑68 (MQ=40)

                     tatttGGCTGGGGAACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTg                                              <  1:8047447/65‑1 (MQ=11)

                     aaagTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                              <  1:16825345‑M2/64‑1 (MQ=255)

                      aagTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg                                             <  1:17821365‑M2/65‑1 (MQ=255)

                       tgTGGCTGGGGTCCGAGGATTCGAACCTCGGAATGCCGGAATCATAAACCGGTGCCTTACCGCTTGGc                                            <  1:56196886‑M2/66‑1 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGATTAAGAATACGGTGCCTTACCGCTagga                                            >  1:52149688‑M2/3‑67 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                   >  1:49594348‑M2/3‑61 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                            >  1:57342695‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                            >  1:56478279‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                            >  1:48406710‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                            >  1:50097783‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                            <  1:25465291‑M2/66‑1 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                            >  1:12846178‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                            >  1:30421526‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                            <  1:35493793‑M2/66‑1 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                            >  1:37855021‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                            >  1:383215‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                            >  1:25131843‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                            >  1:40416294‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCACAAACCGGTGCCTTACCGCTTGGc                                            <  1:2280584‑M2/66‑1 (MQ=255)

                       aTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCATAAACCGGTGCCTTACCGCTTGGc                                            <  1:46908245/68‑1 (MQ=255)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                           >  1:14189993/1‑68 (MQ=31)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                           >  1:57629071/1‑68 (MQ=31)

                                   aCGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaaca                                >  1:16419735/1‑68 (MQ=31)

                                     gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacggg                              >  1:7112050/1‑65 (MQ=1)

                                     gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacagt                              >  1:33026765/1‑66 (MQ=14)

                                      aGGATTCGCACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                             >  1:27797205/1‑68 (MQ=255)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGATTCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                             >  1:34382436/1‑68 (MQ=255)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacatgt                             >  1:34911474/1‑65 (MQ=14)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacagtg                             >  1:11411828/1‑65 (MQ=14)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacaggg                             >  1:37215566/1‑65 (MQ=1)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacaggg                             >  1:44047559/1‑65 (MQ=1)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACaaag                             >  1:46525723/1‑67 (MQ=25)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                             >  1:27119815/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                             >  1:21543550/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                             >  1:20302394/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAAAACTGTGCATTACCGCTGGGCGATACCCCAAGAAAt                             >  1:6300020/1‑68 (MQ=11)

                                       ggATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAtt                            >  1:54508039/1‑68 (MQ=39)

                                       ggATTCGAACCTCGGAATGCCGGAATCAAAAACCTGTGACTTAAAGCTTTGACAGACCCCAAGaaaat                            >  1:42993967/1‑66 (MQ=2)

                                          ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTgg                          <  1:41312313/67‑1 (MQ=255)

                                          ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                         <  1:48724185/68‑1 (MQ=255)

                                          ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                         <  1:18264332/68‑1 (MQ=255)

                                               aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGa                    <  1:30036770/68‑1 (MQ=255)

                                                ccTCCGAAATCCGGAATCAGAATCAGGAGCATTACCGATTCGCGATACACAAACAAAAAGGTTTTGaa                   >  1:4920344/1‑68 (MQ=9)

                                                      aaTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGc              <  1:36051675/67‑1 (MQ=255)

                                                       aTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACGAATTGGTTTTGAATTTGCCg            >  1:26203085/1‑68 (MQ=255)

                                                        tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGGGTTGAATTTGCCGa           >  1:13600595/1‑68 (MQ=255)

                                                         gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGccta           >  1:15271966/1‑65 (MQ=255)

                                                         gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa           >  1:40723082/1‑67 (MQ=255)

                                                           cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAAc         <  1:10046673/67‑1 (MQ=255)

                                                            ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat       <  1:47654753/68‑1 (MQ=255)

                                                            ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat       <  1:856725/68‑1 (MQ=255)

                                                              aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata      <  1:38974310/67‑1 (MQ=255)

                                                               aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat     <  1:23078757/67‑1 (MQ=255)

                                                               aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat     <  1:51664898/67‑1 (MQ=255)

                                                               aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat     <  1:19195159/67‑1 (MQ=255)

                                                               aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat     <  1:53921435/67‑1 (MQ=255)

TACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCG  >  CP009273/691973‑692105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`