BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	692,043	T→A	7.9%	noncoding (30/75 nt)	glnV ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	692,043	0	T	A	7.9%	2609.4 / 83.0	854	noncoding (30/75 nt)	glnV	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base T (515/264); new base A (23/52); total (538/316)
Fisher's exact test for biased strand distribution p-value = 5.09e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 888 total aligned reads displayed.

AAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACA  >  CP009273/691976‑692110

aaGGTAAGCGTCTTGAATAAATTGGCT‑GGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                                                                     >  1:16111260/1‑67 (MQ=255)

aaGGTAAGCGGCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                                                                     <  1:46073685/68‑1 (MQ=255)

 aGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                                                                     >  1:34132705/1‑67 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAtac                                                                   >  1:57984144/1‑66 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:39286329/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:27103586/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:46600111/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:17437697/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:41314707/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:16804036/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:5053730/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:52301480/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:53298440/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:19162048/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:8999393/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:31793634/1‑68 (MQ=255)

  ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAAcc                                                                   >  1:6747399/1‑68 (MQ=255)

    tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                                 >  1:28189285/1‑68 (MQ=255)

    tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGAATCAGAATCCgg                                                                 >  1:45787693/1‑68 (MQ=255)

     accgagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt                                                                <  1:29004268‑M2/51‑1 (MQ=255)

      aGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                >  1:2417507/1‑67 (MQ=255)

      aGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                >  1:12413535/1‑67 (MQ=255)

      aGCGTCTTCAATAAATTGGCTGGGGTAAGAGGATTCGAACAACGGAATGCCGGAAACAGAATACGGt                                                                >  1:49329959/1‑67 (MQ=17)

       cgagcttttttaatcTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGAATCAAAAACCGGTGc                                                              <  1:15666230‑M2/53‑1 (MQ=255)

       ccagctttaataatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                              <  1:30377887‑M2/53‑1 (MQ=255)

        gttCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                             <  1:44416565/66‑1 (MQ=255)

        gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                              <  1:18679776‑M2/53‑1 (MQ=255)

        ccgcTTTAATAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                             <  1:23071382/65‑1 (MQ=11)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:42342332/1‑66 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:19703874/1‑66 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:2972700/1‑66 (MQ=255)

        cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:9714011/1‑66 (MQ=255)

         gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                            >  1:36171612/1‑68 (MQ=255)

         gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                            >  1:37746504/1‑68 (MQ=255)

         gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                            >  1:55981168/1‑68 (MQ=255)

         gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                            >  1:11937183/1‑68 (MQ=255)

         cgcttttataatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAGCAGAATCCGGTGcc                                                             <  1:49879586‑M2/54‑1 (MQ=255)

          tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                           >  1:22092777/1‑68 (MQ=255)

          tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                           >  1:41783451/1‑68 (MQ=255)

          tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGACTCAGAATCCGGTGCCtt                                                           >  1:51440279/1‑68 (MQ=255)

            ttGAATAAATTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGCCTTAc                                                         <  1:56322080/68‑1 (MQ=14)

             tttaTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                        <  1:30797095/65‑1 (MQ=0)

                ttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCACAATCCGGTGCCTTACCGCt                                                     <  1:25395744‑M2/62‑1 (MQ=255)

                aTAAATTGGCTGGGGTACGTGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCt                                                     <  1:56990961/68‑1 (MQ=11)

                aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:31384499/1‑68 (MQ=40)

                aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:17134450/1‑68 (MQ=40)

                aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:3807803/1‑68 (MQ=40)

                  aatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                                   >  1:48986025‑M2/5‑68 (MQ=255)

                   atgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCACAATCCGGTGCCTTACCGCTTgg                                                  <  1:48096896‑M2/65‑1 (MQ=255)

                   aagTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg                                                  <  1:17821365‑M2/65‑1 (MQ=255)

                    tttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGc                                                 <  1:35166650/67‑1 (MQ=11)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGc                                                 <  1:25796555‑M2/66‑1 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGCATCCGGTGCCTTACCGCttgtc                                                 >  1:2489852‑M2/3‑66 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:7359922‑M2/3‑68 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:12846178‑M2/3‑68 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:44133096‑M2/66‑1 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:43382894‑M2/3‑68 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:45152253‑M2/3‑68 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:2350749‑M2/3‑68 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:10570299‑M2/66‑1 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:32357190‑M2/3‑68 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:37165921‑M2/3‑68 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:40456329‑M2/3‑68 (MQ=255)

                    tgTGGCTGGGGTACGAGGATTCGAACCTAGGAATGACGGAAACAGAATCCGGTGCCTTACCGATTGGc                                                 >  1:38790227‑M2/3‑68 (MQ=255)

                    tgTGGCTGGGGTAAGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:50989525‑M2/66‑1 (MQ=255)

                    aTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:48489337/68‑1 (MQ=33)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:20185968/1‑68 (MQ=31)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:33270634/1‑68 (MQ=31)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:20656515/1‑68 (MQ=31)

                     ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:14189993/1‑68 (MQ=31)

                     ttGGCTGGGGTACGAGGATTAGAACCTCGGAATGCCGGAATCAGAATCCGGTGACTTACCGCTTGGCg                                                >  1:46975654/1‑68 (MQ=255)

                                aCGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaaca                                     <  1:57500984/68‑1 (MQ=31)

                                aCGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaaca                                     >  1:16419735/1‑68 (MQ=31)

                                   aGGATTCGAACCTCGGAATGCCGGAGTCAGAATCCGGTGCCTTACCGCTTGGAGATACCCCAACAAAt                                  >  1:53631128/1‑68 (MQ=11)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacagtg                                  >  1:11411828/1‑65 (MQ=14)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:51836818/1‑68 (MQ=37)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:6519578/1‑68 (MQ=37)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:21504795/1‑68 (MQ=37)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:39874239/1‑68 (MQ=37)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:7970589/1‑68 (MQ=37)

                                    ggATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAtt                                 >  1:27791056/1‑68 (MQ=39)

                                    ggATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCC‑ACAAATTg                                >  1:6153081/1‑68 (MQ=255)

                                       ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                              <  1:22731694/68‑1 (MQ=255)

                                            aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGa                         >  1:54530424/1‑68 (MQ=255)

                                                   aaTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGc                   <  1:39619643/67‑1 (MQ=255)

                                                     tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa                <  1:15421479/68‑1 (MQ=255)

                                                     tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa                <  1:47654927/68‑1 (MQ=255)

                                                     tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGGGTTGAATTTGCCGa                >  1:13600595/1‑68 (MQ=255)

                                                      gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGaa               <  1:35848574/68‑1 (MQ=255)

                                                        cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAAc              <  1:10046673/67‑1 (MQ=255)

                                                         ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat            <  1:32903060/68‑1 (MQ=255)

                                                         ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat            <  1:42998809/68‑1 (MQ=255)

                                                         ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat            <  1:856725/68‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:55461897/67‑1 (MQ=255)

                                                                 aaTCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGAt     >  1:18195446/1‑67 (MQ=255)

                                                                 aaTCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGAt     >  1:40840563/1‑67 (MQ=255)

                                                                 aaTCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTCGTTATGAATTTGCCGAACATATTCGAt     >  1:26193910/1‑67 (MQ=255)

                                                                  aTCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATa    <  1:34593949/67‑1 (MQ=255)

                                                                   tCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACa  >  1:53925547/1‑68 (MQ=255)

                                                                   tCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACa  >  1:47357294/1‑68 (MQ=255)

AAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACA  >  CP009273/691976‑692110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`