Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,711,673 A→C 9.0% V72G (GTT→GGT)  yfiF ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,711,6730AC 9.0% 1067.5 / 15.9 393V72G (GTT→GGT) yfiFputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (255/97);  new base C (6/34);  total (261/132)
Fisher's exact test for biased strand distribution p-value = 1.57e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 412 total aligned reads displayed.

AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGG  >  CP009273/2711607‑2711740
                                                                  |                                                                   
aaaCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAAc                                                                    <  1:24019674/68‑1 (MQ=255)
aaaCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAAc                                                                    <  1:13060898/68‑1 (MQ=255)
aaaCTTATACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAAc                                                                    <  1:19228354/68‑1 (MQ=255)
  aCTTTTACCACTGCTGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAACCCg                                                                  >  1:18344152/1‑68 (MQ=255)
  aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                  >  1:10039453/1‑68 (MQ=255)
  aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                  <  1:37811109/68‑1 (MQ=255)
  aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                  >  1:41897688/1‑68 (MQ=255)
  aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                  >  1:31604768/1‑68 (MQ=255)
  aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                  >  1:25557578/1‑68 (MQ=255)
  aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                  >  1:24794745/1‑68 (MQ=255)
  aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                  >  1:21461091/1‑68 (MQ=255)
  aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                  >  1:45870118/1‑68 (MQ=255)
  aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                  >  1:5245864/1‑68 (MQ=255)
  aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                  >  1:11963388/1‑68 (MQ=255)
  aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                  >  1:14207110/1‑68 (MQ=255)
  aCTTTTACAACTGATGCCACCGTGATCGGCCTTTTCCTGCGTCTCATCACCCGGCGCGCGGGAAACCg                                                                  >  1:29038381/1‑68 (MQ=255)
    ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt                                                                 >  1:38220758/1‑67 (MQ=255)
    ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt                                                                 >  1:26263005/1‑67 (MQ=255)
    ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt                                                                 >  1:43942857/1‑67 (MQ=255)
    ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt                                                                 >  1:30308245/1‑67 (MQ=255)
    ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt                                                                 >  1:11314698/1‑67 (MQ=255)
         ccACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCCCCCGGCGCGCGGGAAACCGTGCGcc                                                            >  1:20658781/1‑67 (MQ=255)
          cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCCCCCGGCGCGCGGGAAACCGTGCGcccc                                                          >  1:7436572/1‑66 (MQ=255)
          cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCAc                                                          >  1:26762629/1‑68 (MQ=255)
          cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCAc                                                          >  1:44510987/1‑68 (MQ=255)
          cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCAc                                                          >  1:43379697/1‑68 (MQ=255)
          cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCAc                                                          >  1:35944230/1‑68 (MQ=255)
          cACGGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGccgg                                                          <  1:28495322/68‑3 (MQ=255)
           aCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACg                                                         >  1:19742978/1‑68 (MQ=255)
           aCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACg                                                         >  1:27725174/1‑68 (MQ=255)
                tGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTgcg                                                              >  1:49950816/1‑58 (MQ=255)
                tGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGaa                                                    >  1:13527875/1‑68 (MQ=255)
                  ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGaa                                                    >  1:10697736/1‑66 (MQ=255)
                  ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGaa                                                    >  1:40602861/1‑66 (MQ=255)
                   cACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCt                                                 >  1:30771746/1‑68 (MQ=255)
                   cACCGTGAGCGCCCTTTCCCGGCGTCTCACCCCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCt                                                 <  1:35772058/68‑1 (MQ=255)
                      cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTc                                               >  1:37527154/1‑67 (MQ=255)
                      cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTc                                               >  1:390169/1‑67 (MQ=255)
                      cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTc                                               >  1:27410335/1‑67 (MQ=255)
                      cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTc                                               >  1:24317225/1‑67 (MQ=255)
                          accGGCCTTTCCCGGGGTCTCATCCCCCGCCGCGCGGCAACCGGGGCGCCACGGCGAATCTTCCCACt                                          <  1:50728174/66‑1 (MQ=255)
                          aTCGGCCTTTGCCGGCGTCTCAACCCCCGGCGCGCGGCAAACGGGGCGCCACGGCGAATCTTCCCACt                                          <  1:44369188/68‑1 (MQ=255)
                           tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACtt                                         >  1:25972176/1‑68 (MQ=255)
                           tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACtt                                         >  1:5882533/1‑68 (MQ=255)
                           tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACtt                                         >  1:46470268/1‑68 (MQ=255)
                            cGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACtt                                         >  1:36844940/1‑67 (MQ=255)
                              gCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCg                                      >  1:9712065/1‑68 (MQ=255)
                              gCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCg                                      >  1:16169347/1‑68 (MQ=255)
                               ccTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCgg                                     >  1:24467415/1‑68 (MQ=255)
                               ccTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCgg                                     >  1:7764479/1‑68 (MQ=255)
                                tttttCCGGCTGCTAAACACCCGGGGCGGGGTAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGt                                    <  1:41425565/67‑1 (MQ=255)
                                cTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCgg                                     >  1:21692836/1‑67 (MQ=255)
                                 ttttCCGGGGTCTAATCACCGGGGGCGGGGTAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTc                                   <  1:22887251/68‑1 (MQ=255)
                                 ttttCCGGCGTCTCATCCCCCGGCGCGCGGGAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTc                                   <  1:45546631/68‑1 (MQ=255)
                                 tttcCCGGGGTCTCACCCCCGGGCGCGGGTTAACCGGGGCCCCACGGCGAATCTTCCCACTTGCGGTc                                   <  1:37061642/68‑1 (MQ=255)
                                  tttCCGGCGTCTCAGCCCCGGGCGGGCGGTAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCa                                  <  1:45186154/68‑1 (MQ=255)
                                    tCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACg                                >  1:6515793/1‑68 (MQ=255)
                                     ccGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg                               >  1:35074756/1‑68 (MQ=255)
                                     ccGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg                               >  1:22094414/1‑68 (MQ=255)
                                     ccGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg                               >  1:42161557/1‑68 (MQ=255)
                                     ccGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg                               >  1:3121364/1‑68 (MQ=255)
                                      cGGGGTCTCTCCACCCGGCGGGGGCCAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg                              <  1:32684891/68‑1 (MQ=255)
                                      cGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg                              >  1:34385898/1‑68 (MQ=255)
                                       ggcggcTCATCACCCGGCGCGCGGTAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg                             <  1:33775948/68‑1 (MQ=255)
                                       ggCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg                              >  1:10412947/1‑67 (MQ=255)
                                        gggTCTCATCCCCCGGCGCGCGGCAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg                             <  1:19035891/65‑1 (MQ=38)
                                        gCGTCTCACCACCCGGCGCGGGGGAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg                             <  1:816306/67‑1 (MQ=255)
                                         cGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg                              >  1:2082324/1‑65 (MQ=255)
                                         cGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg                              >  1:9432246/1‑65 (MQ=255)
                                         cGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg                              >  1:23195179/1‑65 (MQ=255)
                                          gTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCt                           >  1:5572912/1‑67 (MQ=255)
                                            ctctTCCCCGGGCGCGCGGTAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa                         <  1:22484039/67‑1 (MQ=255)
                                            ctcACCCCCCGGCGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa                         <  1:7212640/67‑1 (MQ=255)
                                            ctcAACCCCCGGCGCGCGGGAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa                         <  1:47937089/67‑1 (MQ=255)
                                            cacatccCCCGGCGCGGGGCAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa                         <  1:2980423/65‑1 (MQ=38)
                                               atccCCGGGCGCGCGGTAAACGGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct                     <  1:33144069/68‑1 (MQ=255)
                                               atcaCCCGGGGCGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct                     <  1:25309874/68‑1 (MQ=255)
                                               atcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCCCTTGCGGTCACGGGGCTGAc                        >  1:42798205/1‑65 (MQ=255)
                                               accaCCCGGGGCGGTGTAAACGGGGGCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct                     <  1:3957397/66‑1 (MQ=255)
                                                 caCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt                   >  1:3205994/1‑68 (MQ=255)
                                                 caCCCGCCGCGCGGCAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt                   <  1:47505884/68‑1 (MQ=255)
                                                  ccccGGCGCGCGGCAACCGGGCCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct                     <  1:15380817/64‑1 (MQ=255)
                                                  aCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt                  >  1:17974384/1‑68 (MQ=255)
                                                   ccgggCGGGGGGTAACCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt                 <  1:14998053/65‑1 (MQ=255)
                                                   cccGGCGCGGGGGAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt                 <  1:48395406/68‑1 (MQ=255)
                                                   cccGGCGCGCGGGAAACCGTGCGCCCCGGCGAATCTTCCCACTTGCGGTCAGGGGGCTGCCTATGttt                 >  1:49179450/1‑68 (MQ=255)
                                                   cccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt                 >  1:36354796/1‑68 (MQ=255)
                                                   cccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt                 >  1:1705970/1‑68 (MQ=255)
                                                    ccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg                >  1:17457214/1‑68 (MQ=255)
                                                     cGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg                >  1:28322665/1‑67 (MQ=255)
                                                     cGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg                >  1:40208280/1‑67 (MQ=255)
                                                        cgcgcgGTAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCa             <  1:12823585/67‑1 (MQ=255)
                                                        cgcgcgGCAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCa             <  1:12119752/67‑1 (MQ=255)
                                                             gggAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGG       <  1:9014609‑M1/68‑1 (MQ=255)
                                                              ggAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGG       >  1:29399668‑M1/1‑67 (MQ=255)
                                                                 aaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTGACGGGGCTGACTCTGTTTGTCATCgcgggcgg   >  1:31431108/1‑68 (MQ=255)
                                                                  aCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg  >  1:204137/1‑68 (MQ=255)
                                                                  aCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg  >  1:870046/1‑68 (MQ=255)
                                                                  aCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg  >  1:41005156/1‑68 (MQ=255)
                                                                  aCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg  <  1:40029912/68‑1 (MQ=255)
                                                                  |                                                                   
AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGG  >  CP009273/2711607‑2711740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑