| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,263,560 | G→T | 9.5% | intergenic (+599/+15) | yhaC → / ← rnpB | pentapetide repeats‑related protein/RNase P, M1 RNA component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,263,560 | 0 | G | T | 9.5% | 1608.9 / 33.0 | 590 | intergenic (+599/+15) | yhaC/rnpB | pentapetide repeats‑related protein/RNase P, M1 RNA component |
| Reads supporting (aligned to +/- strand): ref base G (334/185); new base T (50/15); total (390/200) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.18e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 594 total aligned reads displayed.
TGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCAT > CP009273/3263493‑3263622 | tGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCggg < 1:34369320/68‑1 (MQ=35) gTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt > 1:512275/1‑68 (MQ=35) gTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt > 1:13541546/1‑68 (MQ=35) gTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt > 1:24809548/1‑68 (MQ=35) gTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtt < 1:378575/67‑1 (MQ=35) gTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtt < 1:33678981/67‑1 (MQ=35) gTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGgtt > 1:26128550/1‑68 (MQ=25) gTATAGCGTCGTGGACAGTCATTCATCTTTCGGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGgtt > 1:21787258/1‑68 (MQ=11) gTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTACAAAAGCAAAAACCCGCCGAAGCGGGttt > 1:14483406/1‑68 (MQ=11) ataGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt > 1:35133844/1‑68 (MQ=35) caacagaaagcggcttatcggtcagtttcacctgttttacgtAAAAACCCGCCGAAGCGGGTTTTTAc > 1:37098550‑M1/43‑68 (MQ=255) aGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc < 1:44738286/68‑1 (MQ=35) gCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg > 1:30131121/1‑68 (MQ=33) gCGTCGTGGACAGTCATTCAACTTTCAGCCCCTACAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg > 1:11622759/1‑68 (MQ=11) cgtcgtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt > 1:10204120/1‑68 (MQ=31) ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCa > 1:29673151/1‑68 (MQ=35) ggACAGTCATTCATCTATCTGCCCCTACAAAAGAAAAAACCCGCCGCAGCCGGTTTTTACGTAACTCa > 1:12542115/1‑68 (MQ=11) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg > 1:4342360/1‑68 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg > 1:31042436/1‑68 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg > 1:14225684/1‑68 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg > 1:7541106/1‑68 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg > 1:1505530/1‑68 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg > 1:15305204/1‑68 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg < 1:42591368/67‑1 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg < 1:41565124/67‑1 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg < 1:47100504/67‑1 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg < 1:20690371/67‑1 (MQ=35) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGACGCGGGTTGTTACGTAAATCAgg > 1:20343977/1‑68 (MQ=11) aCAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg > 1:19509970/1‑68 (MQ=25) aCAGTCATTCATCTTTCTGACCCTCCAAAAGAAAAAACCCGCCGACGCGGGGTTTTACGTAAATCAgg > 1:22405638/1‑68 (MQ=11) gTCATTCATCTTTCTGCCCCTCCGAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGa > 1:30533135/1‑68 (MQ=25) gTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGa > 1:39866304/1‑68 (MQ=35) gTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGa > 1:49235473/1‑68 (MQ=35) gTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGa > 1:10045288/1‑68 (MQ=35) cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg < 1:23899572/63‑1 (MQ=35) cattcatCTTTCTGCCCCTCAAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaa < 1:51019526/67‑1 (MQ=25) tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTg > 1:40201676/1‑68 (MQ=35) ggtcagtttcacctgatttacgtAAAAACCCGCCGTAGCGGGTTTTTACGTAAATCAGGTGAAACTg < 1:9708278‑M1/44‑1 (MQ=255) ggtcagtttcacctgatttacgtAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTg < 1:22117732‑M1/44‑1 (MQ=255) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa > 1:6269609/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa > 1:6915118/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa > 1:22890686/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa > 1:11285088/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa > 1:21533971/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa > 1:31988969/1‑67 (MQ=35) atCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa > 1:16229632/1‑67 (MQ=255) cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCg > 1:42001506/1‑68 (MQ=35) tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGa > 1:12990422/1‑68 (MQ=35) ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGAt > 1:8277616/1‑68 (MQ=35) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAg > 1:44359348/1‑67 (MQ=35) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAg > 1:33863856/1‑67 (MQ=35) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAg > 1:5502623/1‑67 (MQ=35) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAg > 1:2542624/1‑67 (MQ=35) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGc < 1:31513143/68‑1 (MQ=35) cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCg > 1:36364337/1‑68 (MQ=35) ctggtttacgtAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc < 1:50316400‑M1/55‑1 (MQ=255) ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg < 1:8053048/68‑1 (MQ=37) ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg < 1:28260682/68‑1 (MQ=37) tcaaaaaTCATAAACCCGCCGTAACGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGt < 1:21135664/65‑1 (MQ=11) tCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGt < 1:24432728/68‑1 (MQ=255) aaGCACAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGt < 1:42063025/68‑1 (MQ=255) aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGt < 1:48994108/68‑1 (MQ=255) aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGt < 1:45099015/68‑1 (MQ=255) aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGt < 1:33283100/68‑1 (MQ=255) aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGt < 1:32596380/68‑1 (MQ=255) aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGt < 1:42934068/68‑1 (MQ=255) aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGt < 1:8571323/68‑1 (MQ=255) aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGt < 1:28630053/68‑1 (MQ=255) aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGt < 1:23483852/68‑1 (MQ=255) agtAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGGGTTCTgtg < 1:46222114‑M1/65‑2 (MQ=255) aGCAAAAACCCGCCGAAGCGGGTTTTTAGGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTggc > 1:18493995/1‑66 (MQ=255) gCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGGTTCTGtag > 1:11052547/1‑66 (MQ=255) AAAACCCGCCGAACCGGTTTATTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTgg > 1:36884954‑M1/1‑68 (MQ=255) ACCCGCCGTACCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACa > 1:17535543‑M1/1‑68 (MQ=255) ACCCGCCGCAGCGGGTTATTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACa > 1:44060885‑M1/1‑68 (MQ=255) ACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACa > 1:10639600‑M1/1‑68 (MQ=255) ACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACa > 1:37677721‑M1/1‑68 (MQ=255) ACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGGTTTCTGTGGTggaga < 1:35761232‑M1/68‑3 (MQ=255) gCGCAGAAGCGTGTTTTTAAGAAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt > 1:40803613‑M1/2‑68 (MQ=255) CCGCCTAACCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt > 1:48137685‑M1/1‑68 (MQ=255) CCGCCGTAGCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt > 1:7796677‑M1/1‑68 (MQ=255) CCGCCGTACCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt > 1:9114489‑M1/1‑68 (MQ=255) CCGCCGCAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGATAGt > 1:27609455‑M1/1‑68 (MQ=255) CCGCCGAATCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt > 1:12126328‑M1/1‑68 (MQ=255) CCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt > 1:41242607‑M1/1‑68 (MQ=255) CCGCCGAAGCGGGTTATTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt > 1:34671043‑M1/1‑68 (MQ=255) CCGCCGAACCGGTTTATTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt > 1:38763401‑M1/1‑68 (MQ=255) CCGCCGAACCGGTTTATTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt > 1:48731848‑M1/1‑68 (MQ=255) CCGCCGAACCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt > 1:49743271‑M1/1‑68 (MQ=255) CGCCGCAGCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTc > 1:26898608‑M1/1‑68 (MQ=255) CGCCGCACCGGTTTTTTACGTACATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTc > 1:26470254‑M1/1‑68 (MQ=255) CGCCGAAGCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTc > 1:38146987‑M1/1‑68 (MQ=255) CGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTc > 1:45685493‑M1/1‑68 (MQ=255) CGCCGAACCGGTTTATTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTc > 1:16649321‑M1/1‑68 (MQ=255) GCCGAAGCGGGTTTTTTCGTAAATCACGTGAAACTGCCCTATAAGCCGGGTTCTGTCGTGGACAGTCa > 1:15662045‑M1/1‑68 (MQ=255) GCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCa > 1:17123099‑M1/1‑68 (MQ=255) aGCGGTTTATTACGTACATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattca < 1:2731204/67‑1 (MQ=18) aGCGGGTTTTTAGGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattcat < 1:18123695/68‑1 (MQ=255) aGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattcat < 1:40512201/68‑1 (MQ=255) aGCGGGTTTTTACGTAAATCACGTGAAACTTACCTATAAGCCGTTTTCTGTCGTGGACAGtcattcat < 1:39228396/68‑1 (MQ=18) | TGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCAT > CP009273/3263493‑3263622 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |