| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 122,271 | C→T | 5.9% | A30A (GCC→GCT) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 122,271 | 0 | C | T | 5.9% | 2520.2 / 71.6 | 793 | A30A (GCC→GCT) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base C (241/478); new base T (2/44); total (267/526) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.02e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 821 total aligned reads displayed.
GGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGT > CP009273/122205‑122337 | aCATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGCAGCAGa > 1:46184267/1‑67 (MQ=255) aCATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGa > 1:10287894/1‑67 (MQ=255) aCATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGa > 1:33570319/1‑67 (MQ=255) aCATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGa > 1:30081542/1‑67 (MQ=255) aCATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGa > 1:42269789/1‑67 (MQ=255) aCATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGa < 1:31948360/67‑1 (MQ=255) aCATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGa > 1:1620348/1‑67 (MQ=255) aCATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGa > 1:28325269/1‑67 (MQ=255) aCATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGa > 1:17440180/1‑67 (MQ=255) aCATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCAGa > 1:12370430/1‑67 (MQ=255) aTCGGGGCTGATGAAGTTGAATTCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACa > 1:6094426/1‑68 (MQ=255) aTCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGCAGCTGAACa > 1:39008355/1‑68 (MQ=255) aTCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACa > 1:24639713/1‑68 (MQ=255) aTCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACa < 1:30891379/68‑1 (MQ=255) tCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAg < 1:5378513/68‑1 (MQ=255) tCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCAGAACAg > 1:8679310/1‑68 (MQ=255) cGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGt < 1:6977220/68‑1 (MQ=255) cGGGGCGGATGAAGTTGAAATCACCGAGAGCCTGGTCAAAGTGGGCGCCAAAGTTGAAGCCGAACAGt < 1:16037657/68‑1 (MQ=255) ggggCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTc > 1:22357554/1‑68 (MQ=255) ggggCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTc < 1:2170752/68‑1 (MQ=255) ggggCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTc < 1:45785229/68‑1 (MQ=255) ggggCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTc < 1:25350189/68‑1 (MQ=255) ggggCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTc < 1:20354502/68‑1 (MQ=255) ggggCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTc < 1:74716/68‑1 (MQ=255) ggggCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTc < 1:13333610/68‑1 (MQ=255) ggggCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTc < 1:1293264/68‑1 (MQ=255) ggggCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTc < 1:37740723/68‑1 (MQ=255) cgggCTGTTGAAGTTGAATTCTCCGAGATCCTGGTCAAAGTGGGGGACAAAGTTGAATCCGAACAGTc < 1:21216003/67‑1 (MQ=255) gggCTGATGAAGTTGAAATCACCGAGATGCTGGTCCAAGTGGGCGACAAAGTTGAAGCACAACAGTc > 1:30597846/1‑67 (MQ=37) gggCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTc > 1:16710579/1‑67 (MQ=255) gCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGCAGCCGAACAGTc > 1:46872424/1‑65 (MQ=255) gCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTc > 1:20793962/1‑65 (MQ=255) gCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTc > 1:28756567/1‑65 (MQ=255) gCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGc < 1:27010771/67‑1 (MQ=255) gCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGc < 1:3636888/67‑1 (MQ=255) gCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGc < 1:46489988/67‑1 (MQ=255) tgatgaAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGa < 1:33840217/68‑1 (MQ=18) tgatgaAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTg < 1:49246062/67‑1 (MQ=255) tgatgaAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGa < 1:43527688/68‑1 (MQ=255) tgaAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGCACAGTCGCTGATc > 1:44496794/1‑67 (MQ=255) tgaAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATc < 1:19540457/67‑1 (MQ=255) tgaAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATc < 1:11648752/67‑1 (MQ=255) tgaAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATc < 1:915094/67‑1 (MQ=255) tgaAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCAGAACAGTCGCTGATc > 1:25939107/1‑67 (MQ=255) tgaAGTTGAAATCACCGACATCCTGGTCAAAGTTGGCGACAAAGTTGAAGCTGAACAGTCGCTGATCa < 1:14436434/68‑1 (MQ=14) gaAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCAc < 1:32468271/68‑1 (MQ=255) aGTTGAATTCACCGAGATGCTGGTCAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCAcc < 1:9566093/67‑1 (MQ=11) aGTTGAAATCACCGATATCCTGGTCAAAGTGGGCGAAAAAGTTGAAGCCGAACAGTCGCTGATCACCg < 1:23079333/68‑1 (MQ=255) aGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCAcc < 1:24961982/67‑1 (MQ=255) aGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCAcc < 1:38564856/67‑1 (MQ=255) aGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCAcc < 1:17956386/67‑1 (MQ=255) aGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCAcc < 1:18091970/67‑1 (MQ=255) aGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCAcc < 1:24157363/67‑1 (MQ=255) aGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCg < 1:37363891/68‑1 (MQ=255) ttGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTa > 1:8203953/1‑68 (MQ=255) tGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGt < 1:43077450/66‑1 (MQ=255) aTCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAg > 1:29825751/1‑67 (MQ=255) tCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGc > 1:10745724/1‑68 (MQ=255) cACCGATATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCTGAACAGTc < 1:19934772/48‑1 (MQ=17) cccGAGAGCCAGGTCAAAGTGGGCGACAAAGTAGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGa < 1:348042/67‑1 (MQ=18) aCCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGa < 1:15651131/68‑1 (MQ=255) ccGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGaa > 1:47451102/1‑62 (MQ=255) gagaTCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACaa < 1:41262866/68‑1 (MQ=255) agaTCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACaag > 1:23662993/1‑67 (MQ=255) aTCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACaa > 1:31164122/1‑65 (MQ=255) tCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAg < 1:41024816/66‑1 (MQ=255) tCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAg < 1:50388935/66‑1 (MQ=255) tCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGcc < 1:26362337/68‑1 (MQ=255) tCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGcc < 1:51095205/68‑1 (MQ=255) tCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGcc < 1:18815407/68‑1 (MQ=255) tCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGcc < 1:39219544/68‑1 (MQ=255) tCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGcc < 1:27752986/68‑1 (MQ=255) ccTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGcg > 1:35684270/1‑66 (MQ=255) ccTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGct > 1:39603861/1‑66 (MQ=255) ccTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCct < 1:49641860/68‑1 (MQ=255) ccTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCct < 1:44943440/68‑1 (MQ=255) ccTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCct < 1:39941900/68‑1 (MQ=255) cTGGTCAAAGTGGGCGACAAAGTTGAACCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCctc < 1:35224490/68‑1 (MQ=255) tGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCctct < 1:44260210/68‑1 (MQ=255) gTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTAt > 1:17186047/1‑68 (MQ=255) tCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATg > 1:43856493/1‑68 (MQ=255) tCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTAt < 1:11326651/67‑1 (MQ=255) tCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATg > 1:47832504/1‑68 (MQ=255) cAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCGTCTATgg > 1:32751876/1‑68 (MQ=255) cAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATg < 1:6557410/67‑1 (MQ=255) cAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATg < 1:47226833/67‑1 (MQ=255) gTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGt > 1:27301473/1‑68 (MQ=255) gggCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGt < 1:2913446/66‑1 (MQ=255) gACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGtc < 1:138629/68‑1 (MQ=255) cAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGtctc < 1:36837617/68‑1 (MQ=255) aaGTTGAAGCCGAACAGTCGCTGATCACCCTAGAAGGCGACAAAGAGTCTATGGAAGTTCAGTCTcc > 1:42562359/1‑67 (MQ=14) gTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGctt < 1:22149550/46‑3 (MQ=255) tGAAGCCGAACAGTCGCTGTTCACCGTAGAAGGCGACAAAGCCTCTATGTAAGTTCCGTCTCCGCAgg < 1:48771145/68‑1 (MQ=18) tGAAGCCGAACAGTCGCTGATCACCGTAGACGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAgg < 1:33288730/68‑1 (MQ=255) tGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAgg < 1:22714246/68‑1 (MQ=255) tGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAgg < 1:18319917/68‑1 (MQ=255) tGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAg > 1:14818270/1‑67 (MQ=255) cGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCggg < 1:41724319/66‑1 (MQ=255) cGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCggg < 1:34422822/66‑1 (MQ=255) cGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTATATGGAAGTTCCGTCTCCGCAGGCggg < 1:45370461/66‑1 (MQ=255) | GGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGT > CP009273/122205‑122337 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |