| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,300,453 | T→G | 15.4% | N256T (AAC→ACC) | deaD ← | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,300,453 | 0 | T | G | 15.4% | 1496.7 / 77.2 | 662 | N256T (AAC→ACC) | deaD | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base T (281/271); new base G (108/2); total (389/273) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.38e-25 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 728 total aligned reads displayed.
GTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCT > CP009273/3300386‑3300518 | cACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGtt < 1:21434654/67‑1 (MQ=255) cACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGtt < 1:28932367/67‑1 (MQ=255) gTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTgg < 1:32686794/68‑1 (MQ=255) tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTGGGt < 1:26430048/67‑1 (MQ=255) tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGt > 1:24183019/1‑67 (MQ=255) aGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTacg > 1:27709478/1‑68 (MQ=255) gcgtcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:23405268/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTTGTacgaac > 1:12408564/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaac > 1:13560982/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaac > 1:35640565/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaac > 1:16847472/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaac > 1:25099155/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:45347429/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:35005884/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:15717382/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:16154784/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:18871511/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:20593832/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:36702323/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacga < 1:36239117/66‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGATTCAGCCACTTCCAGAGTCGAGGTTTTGGTacgaac > 1:47433641/1‑68 (MQ=255) ggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaacga < 1:46569997/68‑1 (MQ=255) gcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGa < 1:45770662/68‑1 (MQ=255) gcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGa < 1:19366168/68‑1 (MQ=255) gcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGa < 1:18411453/68‑1 (MQ=255) tgtggtAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATa < 1:15269468/68‑1 (MQ=255) tgtAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGGAGATAATc > 1:49525126/1‑68 (MQ=255) aGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGGTCGCACGCAGCTAATcgc > 1:31893178/1‑67 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTTGTACGAACCAAAATAATagcccc > 1:38556563/1‑66 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTTCGAACGAAGATAATcgccgc > 1:27383470/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTTCGAACGAAGATAATcgccgc > 1:46196953/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGCACGAAGATAATcgccgc > 1:41388175/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGCACGAAGATAATcgccgc > 1:50197121/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAATATAATagccg > 1:43340712/1‑67 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:34374480/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:3734569/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:24503084/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccg > 1:14758611/1‑67 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAAAcgccgg > 1:9604750/1‑67 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGGACGCACGAAGGTTATcgccg > 1:48787341/1‑67 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTGTTTTGTACGAACGAAGAAAATagccgc > 1:47115422/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTTGTACGCACGAAGAAAATcgccgc > 1:20024749/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTTCGCACGAAGATATTcgccgc > 1:48100636/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTACGAACGAAGATAATcggcg > 1:33011794/1‑67 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTACGAACGAAGATAATcgccgc > 1:18183431/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTACCAACCAATAAAAAcgcccc > 1:26067676/1‑66 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGGTCGCACGCAGATAATagcc > 1:42725010/1‑66 (MQ=255) cGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCa < 1:44485684/68‑1 (MQ=255) aCGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCaa < 1:22490053/68‑1 (MQ=255) aCGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCaa < 1:23994699/68‑1 (MQ=255) tCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTTGTAAGAACGAAGATAATCGCCGCATCaaaa > 1:7867450/1‑66 (MQ=255) tCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAAt < 1:31451621/67‑1 (MQ=255) tCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAAt < 1:21013506/67‑1 (MQ=255) tCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAAt < 1:46672497/67‑1 (MQ=255) tCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAAt < 1:39844010/67‑1 (MQ=255) tCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATAGCCGCATCAAAAta > 1:10047662/1‑67 (MQ=255) tCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGGACGCACGCAGATAATCGCCGCAGCaaaa > 1:12624392/1‑66 (MQ=255) agagCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAGCGAAGATAAACGCCGCATCAAAAtaat > 1:42313029/1‑64 (MQ=255) agagCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCtt > 1:4499181/1‑67 (MQ=255) agagCTTCAGCCACTTCCAGAGTCGCGGGTTTGGTTCGCAACAAGATAATAGCCGCATCAAAATAtt > 1:40600923/1‑67 (MQ=255) gagCTTCAGCCACTTCCAGAGTCGCGTTTTTTGTACGAACGAAGATAATCGCCGCATCAAAATCttac > 1:8130099/1‑66 (MQ=255) agCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATAGCCGCATCAAAATAttac > 1:30645953/1‑65 (MQ=255) gCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCCTCAAAATCTTcc > 1:33687550/1‑66 (MQ=255) gCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATAttgc > 1:29277483/1‑64 (MQ=255) gCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATAGCCGCATCAAAATCTTACGc > 1:43892343/1‑68 (MQ=255) gCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATAGCCGCAACAAAAACttac > 1:43613043/1‑64 (MQ=255) gCTTCAGCCACTTCCAGAGTCGCGGTTTTTGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGc > 1:27053316/1‑68 (MQ=255) ttCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCt < 1:5367993/67‑1 (MQ=255) ttCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACCAAGATAAAAGCCCCAACAAAATATTCCGCt > 1:38066564/1‑67 (MQ=255) tCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTc < 1:13955508/68‑1 (MQ=255) tCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTc < 1:21939014/68‑1 (MQ=255) tCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTc < 1:30897484/68‑1 (MQ=255) cAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTcc < 1:28551451/68‑1 (MQ=255) cAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTcc < 1:15070783/68‑1 (MQ=255) cAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTcc < 1:34699874/68‑1 (MQ=255) gCCACTTCCAGAGTCGCGTTTTTTGTACGCACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAg > 1:17880061/1‑68 (MQ=255) ccACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATAAAAATCTTCCGCTTCCAgg > 1:5579950/1‑68 (MQ=255) cACTTCCAGAGTCGCGTTTTTGGTTCGAACGAAGAAAAAAGCCCCATCAAAATCTTACGCTTGCACGa > 1:14223886/1‑68 (MQ=255) aCTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGaa < 1:19658733/68‑1 (MQ=255) ttCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAAc < 1:4179401/68‑1 (MQ=255) ttCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAAc < 1:29717620/68‑1 (MQ=255) ttCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAAc < 1:25451244/68‑1 (MQ=255) ttCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAAc < 1:6771442/68‑1 (MQ=255) ttCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAAc < 1:6094501/68‑1 (MQ=255) ttCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAAc < 1:49882518/68‑1 (MQ=255) ttCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAAc < 1:38828842/68‑1 (MQ=255) ttCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAAc < 1:14515933/68‑1 (MQ=255) tCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACg < 1:33454212/68‑1 (MQ=255) ccAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGt < 1:8859010/68‑1 (MQ=255) cAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTa < 1:12033883/68‑1 (MQ=255) cAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTa < 1:40185597/68‑1 (MQ=255) agTCGCGTTTTTTGTACGAACGAAGATAAAACCCGCATCAAAAACTTACGCTTCCAGGAAACGTAACa > 1:32062975/1‑68 (MQ=255) agTCGCGTTTTTGGTACGAACGAATATAAAAGCCGCATCAAAAAATTCCGCTTCCAGGAAACCTAACa > 1:48418560/1‑68 (MQ=255) agTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCa > 1:23084242/1‑68 (MQ=255) agTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCa > 1:9142189/1‑68 (MQ=255) cgcgTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGt < 1:11193340/67‑1 (MQ=255) cgcgTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGt < 1:36451584/67‑1 (MQ=255) cgcgTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGt < 1:7123007/67‑1 (MQ=255) cgcgTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGt < 1:30170370/67‑1 (MQ=255) gcgTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGc < 1:10752583/68‑1 (MQ=255) | GTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCT > CP009273/3300386‑3300518 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |